The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Microscopy Research and Technique, Journal Year: 2024, Volume and Issue: 87(5), P. 1092 - 1110

Published: Jan. 22, 2024

Abstract In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores combined effects caffeine, a common pharmaceutical pollutant, MPs on marine mussel Mytilus galloprovincialis . Caffeine, at concentrations 20.0 μg L −1 , (1 mg 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. specimens were divided into four groups: MPs, Mix (caffeine + MPs), Control. After two‐week acclimation period, mollusks subjected these pollutants oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments performed evaluate gill morphology. Cellular volume regulation digestive gland cell viability also analyzed. Exposure caffeine induced significant morphological changes gills, including cilia loss, ciliary disk damage, cellular alterations. The chitinous rod supporting filaments suffered potentially due MP interactions, leading hemocyte infiltration filament integrity compromise. Hemocytic aggregation suggested an inflammatory response caffeine. addition, cells revealed potential damage membranes function, with impaired regulation, particularly group, raising nutrient metabolism disruption organ function These findings underscore vulnerability emphasize need monitoring mitigation efforts. Research Highlights synergy warrants investigation. could affect morphology Caffeine‐exposed had lower than control group NR retention test. mix‐exposed struggled recover volume.

Language: Английский

---

Polystyrene Micro- and Nanoplastics (PS MNPs): A Review of Recent Advances in the Use of -Omics in PS MNP Toxicity Studies on Aquatic Organisms

Fishes, Journal Year: 2024, Volume and Issue: 9(3), P. 98 - 98

Published: March 5, 2024

In recent years, micro- and nanoplastics (MNPs) have represented an emerging threat for the aquatic environment due to their persistence widespread distribution. Indeed, small size increased surface area lead a high biological reactivity, which can be crucial health status of biota. To date, several studies investigated lethal sublethal endpoints MNPs, while one main challenges is understand mechanisms toxicity in comprehensive manner. With this aim, field ecotoxicology now benefit from -omics approaches. This review focuses on advances related use transcriptomics, proteomics, metabolomics deeply investigate molecular biochemical responses organisms, both fishes invertebrates, pristine polystyrene (PS) MNPs. The literature reviewed revealed that transcriptomics are most frequently used -omic Overall, taken into consideration shed light events triggered by PS MNPs at cellular levels, identifying as pathways involved oxidative stress, energy metabolism, immune response, nervous system. Future should therefore focus integration multi-omics approaches more understanding toxicity.

Language: Английский

---

Unraveling the interplay between environmental microplastics and salinity stress on Mytilus galloprovincialis larval development: A holistic exploration

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 927, P. 172177 - 172177

Published: April 3, 2024

Language: Английский

---

Toxicity assessment of animal manure composts containing environmental microplastics by using earthworms Eisenia andrei

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 931, P. 172975 - 172975

Published: May 3, 2024

Nowadays, animal manure composting constitutes a sustainable alternative for farmers to enhance the level of nutrients within soils and achieve good productivity. However, pollutants may be present in manures. This study focuses on detection environmental microplastics (EMPs) into composts, as well assessment their potential toxicity earthworm Eisenia andrei. To these aims, animals were exposed two types compost, namely bovine (cow) ovine (sheep) manure, besides mixture, 7 14 days. The presence characterization EMPs was evaluated all tested tissues earthworms. impact composts assessed by multi-biomarker approach including cytotoxic (lysosomal membrane stability, LMS), genotoxic (micronuclei frequency, MNi), biochemical (activity catalase, CAT, glutathione-S-transferase, GST; content malondialdehyde, MDA), neurotoxic acetylcholinesterase, AChE) responses Results indicated high levels especially sheep (2273.14 ± 200.89 items/kg) comparison cow (1628.82 175.23 items/kg), with size <1.22 μm most abundant EMPs. A time-dependent decrease LMS AChE noted earthworms, concomitant increase DNA damages (MNi) after days exposure. Also, severe oxidative stress recorded treated different compost through an CAT GST activities, LPO levels, Therefore, it is necessary carefully consider findings agricultural practices terms plastic mitigation usage, order prevent any risk environment health.

Language: Английский

---

Metabolomic and biochemical disorders reveal the toxicity of environmental microplastics and benzo[a]pyrene in the marine polychaete Hediste diversicolor

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 477, P. 135404 - 135404

Published: Aug. 2, 2024

Language: Английский

---

Transcriptional responses of Mediterranean mussels (Mytilus galloprovincialis) under the 2022 Marine Heatwave: a trade-off of physiological regulation between metabolism, stress response, and shell biomineralization in a mixed exposure scenario

Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126328 - 126328

Published: April 1, 2025

There has been a notable increase in occurrence and intensity of marine heatwaves (MHW) over the past decades, with consequent remarkable risk to vulnerable species as bivalves. This study examines responses farmed Mytilus galloprovincialis 2022 MHW that impacted Northwestern (NW) Adriatic Sea. Expression key transcripts involved functions digestive glands mantles were investigated explore putative acclimatory processes contributing mussel fitness. The was characterized by persistent sea temperature anomalies, elevated salinity, dramatically low chlorophyll-a levels. Despite temporal trends pH extreme seawater temperatures reached July August, carbonate system never undersaturation state, being favourable for bivalve biomineralization. Transcriptional profiles displayed 2-step response. In glands, metabolism lysosomal response functional categories showed an initial decrease (late May), recovery late August. Antioxidant cytoprotective related gene products February August increased expression, strong up-regulations mantles, shell biomineralization prompted stage MHW, likely withstand abrupt changes parameters maintain growth. At high intensities, energy diverted towards stress activation relative mRNA levels transcripts. Results trade-off between core physiological may contribute mussels cope adverse conditions NW

Language: Английский

---

Microplastics’ Impact on the Environment and the Challenging Selection of Reliable Key Biomonitors

Water, Journal Year: 2024, Volume and Issue: 16(18), P. 2637 - 2637

Published: Sept. 17, 2024

Microplastics (MPs) persist for long periods in the environment, causing adverse effects on aquatic and terrestrial ecosystems. The accumulation of MPs various trophic levels mostly depends weathering phenomena, their reduced dimensions improved bioavailability; this ultimately causes ingestion by organisms living different niches. modern concern about toxicity collides with current unavailability standardized reliable methodologies to assess risks associated exposure from habitats. Hence, identification selection appropriate biomonitors pollution risk assessment should focus easy-to-implement assays, rapidly interpretable results (e.g., based bioaccumulation capabilities tissues) standardizable methodologies. present review analyzed some emerging exploited evaluation, selected examined according potential use as specific biological indicators diverse environments. research was focused plants, models airborne microfibers evaluation; mussels, key establishment marine environments; land snails, representing studies MPs’ impact soil. Furthermore, recent findings evidenced influence microplastics composition environmental microbiota, enhancing pathogenic biofilms formation, leading increased water, soil, food, crops waste contamination. Disposing harmonized validated methods study integrated promising machine learning tools, might sensibly support management strategies protecting human animal health.

Language: Английский

---

Using marine mussels to assess the potential ecotoxicological effects of two different commercial microplastics

Marine Pollution Bulletin, Journal Year: 2024, Volume and Issue: 203, P. 116441 - 116441

Published: May 4, 2024

Language: Английский

---

A metabolomics perspective on the effect of environmental micro and nanoplastics on living organisms: A review

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 932, P. 172915 - 172915

Published: May 6, 2024

The increasing trend regarding the use of plastics has arisen an exponential concern on fate their derived products to environment. Among these derivatives, microplastics and nanoplastics (MNPs) have been featured for associated environmental impact due low molecular size high surface area, which prompted ubiquitous transference among all interfaces. Due heterogenous chemical composition MNPs, study particles focused a number studies, as result myriad physicochemical properties that contribute co-transference wide range contaminants, thus becoming major challenge scientific community. In this sense, both primary secondary MNPs are well-known be adscribed industrial urbanized areas, from they massively released environment through multiscale level, involving atmosphere, hydrosphere, lithosphere. Consequently, much research conducted understanding interconnection between those interfaces, motivate spread contaminants biological systems, being mostly represented by biosphere, especially phytosphere and, finally, anthroposphere. These findings highlighted potential hazardous risk human health different mechanisms environment, requiring deeper approach define real exposure. As result, there is gap knowledge high-throughput perspective. review, metabolomics-based overview interfaces was proposed, considering technology highly valuable tool decipher opening novel perspective contaminants.

Language: Английский

---

The Effects of Tetrabromobisphenol A (TBBPA) on the Mussel Mytilus galloprovincialis: A Multi-Biomarker Approach

Oceans, Journal Year: 2024, Volume and Issue: 5(2), P. 181 - 195

Published: April 3, 2024

Tetrabromobisphenol A (TBBPA) is a fire-retardant containing bromine, produced in large quantities worldwide and extensively used several industrial products. This compound was identified as potential contaminant of the environment, causing toxicity to organisms. However, its remains poorly understood marine bivalves. The first objective this work evaluate impact TBBPA on mussels (Mytilus galloprovincialis) exposed for 28 days various concentrations (0, 1, 10, 100 µg·L−1), by assessing stress biomarkers’ responses (Glutathione S-transferase, superoxide dismutase, catalase, lipid peroxidation, total antioxidant capacity, ubiquitin, caspase-3 acetylcholinesterase). results showed that lower (1 10 µg·L−1) were efficiently detoxified, suggested GST activities, which supported other biomarkers. most pronounced effects observed animals highest concentration (100 suggesting oxidative stress. Additionally, significant strong correlations found between capacity some biomarkers (superoxide dismutase peroxidation), showing processes involved fighting are working avoid cell injury. In brief, mussels’ defense mechanisms capable dealing with exposure tested. Despite this, risk consuming shellfish or fishery products contaminated should be cause concern.

Language: Английский

---