International Immunopharmacology, Journal Year: 2024, Volume and Issue: 136, P. 112306 - 112306
Published: June 3, 2024
Language: Английский
Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)
Published: Oct. 2, 2023
The mammalian target of rapamycin (mTOR) is a protein kinase that controls cellular metabolism, catabolism, immune responses, autophagy, survival, proliferation, and migration, to maintain homeostasis. mTOR signaling cascade consists two distinct multi-subunit complexes named complex 1/2 (mTORC1/2). catalyzes the phosphorylation several critical proteins like AKT, C, insulin growth factor receptor (IGF-1R), 4E binding 1 (4E-BP1), ribosomal S6 (S6K), transcription EB (TFEB), sterol-responsive element-binding (SREBPs), Lipin-1, Unc-51-like autophagy-activating kinases. plays central role in regulating translation, lipid synthesis, nucleotide biogenesis lysosomes, nutrient sensing, signaling. emerging pieces evidence have revealed constitutive activation pathway due mutations/amplification/deletion either its (mTORC1 mTORC2) or upstream targets responsible for aging, neurological diseases, human malignancies. Here, we provide detailed structure mTOR, complexes, comprehensive regulators, as well downstream effectors cascades biomolecules, autophagy. Additionally, summarize potential long noncoding RNAs (lncRNAs) an important modulator Importantly, highlighted disorders, cancers, cancer stem cells, drug resistance. discuss developments therapeutic targeting with improved anticancer efficacy benefit patients clinics.
Language: Английский
Citations
329
Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
The invasion and metastasis of pancreatic cancer (PC) are key factors contributing to disease progression poor prognosis. This process is primarily driven by EMT, which has been the focus recent studies highlighting role long non-coding RNAs (lncRNAs) as crucial regulators EMT. However, mechanisms lncRNAs influence invasive multifaceted, extending beyond EMT regulation alone. review aims characterize affecting in cancer. We summarize regulatory roles across multiple molecular pathways highlight their translational potential, considering implications for clinical applications diagnostics therapeutics. focuses on three principal scientific themes. First, we orchestrate various signaling pathways, such TGF-β/Smad, Wnt/β-catenin, Notch, regulate changes associated with thereby enhancing cellular motility invasivenes. Second, effects autophagy ferroptosis discuss exosomal tumor microenvironment behavior neighboring cells promote cell invasion. Third, emphasize RNA modifications (such m6A m5C methylation) stabilizing capacity mediate PC. Lastly, potential these findings, emphasizing inherent challenges using biomarkers therapeutic targets, while proposing prospective research strategies.
Language: Английский
Citations
1
CNS Neuroscience & Therapeutics, Journal Year: 2025, Volume and Issue: 31(2)
Published: Feb. 1, 2025
ABSTRACT Background Glioma stem cells (GSCs) contribute to the initiation, recurrence, metastasis, and drug resistance of glioblastoma multiforme (GBM). Long noncoding RNAs (lncRNAs) are critical modulators in development progression GBM; however, specific lncRNAs related GSCs remain largely unexplored. This study aims identify dysregulated GSCs, unravel their contributions GBM progression, propose new targets for diagnosis treatment. Methods GeneChip analysis was utilized GSCs. The expression examined using quantitative real‐time PCR. Cell Counting Kit‐8, tmorsphere formation assay, limiting dilution apoptosis detection intracranial xenograft models were performed assess stemness radioresistance Transcriptomics analysis, RNA immunoprecipitation dual‐luciferase experiments conducted mechanistic studies. Results NONHSAT141192.2 exhibited elevated levels aggressive tissues compared lower‐grade gliomas. Silencing resulted a considerable decrease GSC proliferation, tumor sphere formation, self‐renewal key cell markers. Furthermore, depletion enhanced sensitivity radiation, indicated by diminished viability tumorsphere increased apoptosis, decreased growth models. Mechanistically, upregulates SOX2 PIK3R3 sponging miR‐4279, influencing characteristics radiation. Conclusion highlights significant relationship between NONHSAT141192.2, stemness, radioresistance, emphasizing its potential as therapeutic target treatment radiosensitization.
Language: Английский
Citations
0
Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 253, P. 155044 - 155044
Published: Dec. 20, 2023
Language: Английский
Citations
10
Biochimica et Biophysica Acta (BBA) - Reviews on Cancer, Journal Year: 2025, Volume and Issue: 1880(3), P. 189323 - 189323
Published: April 15, 2025
Language: Английский
Citations
0
BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)
Published: Jan. 15, 2024
Abstract Background GEFT is a key regulator of tumorigenesis in rhabdomyosarcoma (RMS), and overexpression significantly correlated with distant metastasis, lymph node poor prognosis, yet the underlying molecular mechanism still poorly understood. This study aimed to investigate validate GEFT-activated lncRNAs regulating mTOR expression promote progression RMS. Methods GEFT-regulated were identified through microarray analysis. The effects on proliferation, apoptosis, invasion, migration RMS cells confirmed cell functional experiments. target miRNAs regulation predicted by bioinformatics analysis combined quantitative real-time polymerase chain reaction (qRT–PCR) lnc-PSMA8-1, miR-144-3p, was measured qRT–PCR tissue samples lines. regulatory mechanisms lnc-PSMA8-1-miR-144-3p-mTOR signaling axis verified RNA-binding protein immunoprecipitation (RIP), luciferase reporter assay, analysis, Western blot Results microarray-based 31 differentially expressed (fold change > 2.0, P < 0.05). Silencing 4 upregulated (lnc-CEACAM19-1, lnc-VWCE-2, lnc-GPX7-1, lnc-PSMA8-1) overexpressing downregulated lnc-FAM59A-1 inhibited induced apoptosis cells. Among factors analyzed, consistent correlations among their indicated lncRNA–miRNA–mRNA network based ceRNA hypothesis. lnc-PSMA8-1 promoted competitively binding miR-144-3p regulate expression. Conclusion Our research demonstrated that activated former positively regulated sponging Therefore, targeting this may constitute potential therapeutic approach for management
Language: Английский
Citations
2
Archives of Oral Biology, Journal Year: 2023, Volume and Issue: 158, P. 105855 - 105855
Published: Nov. 28, 2023
Language: Английский
Citations
4
Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(6), P. 3046 - 3061
Published: June 1, 2024
The long non-coding RNA (lncRNA) Opa interacting protein 5-antisense 1 (OIP5-AS1) has been shown to participate in numerous biological and pathological processes, notably including oncogenesis. OIP5-AS1 modulates oncogenic or anti-tumor activities by controlling various microRNAs (miRs) diverse cancer types. This study sought examine the potential role of lncRNA OIP5-AS1-mediated miR-455-3p/microfibril-associated 2 (MFAP2) axis its influence on progression thyroid carcinoma.
Language: Английский
Citations
1
Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(8), P. 4113 - 4130
Published: Aug. 1, 2024
The recently identified carcinogenic long non-coding RNA (lncRNA) MIR4435-2HG has been validated to contribute the initiation and progression of several malignancies. Nonetheless, its specific mechanistic function in pancreatic cancer (PC) is yet be determined. This study aims investigate expression functional role PC elucidate potential mechanism.
Language: Английский
Citations
1
Database, Journal Year: 2024, Volume and Issue: 2024
Published: Jan. 1, 2024
Cancer stemness plays an important role in cancer initiation and progression, is the major cause of tumor invasion, metastasis, recurrence, poor prognosis. Non-coding RNAs (ncRNAs) are a class RNA transcripts that generally cannot encode proteins have been demonstrated to play critical regulating stemness. Here, we developed ncStem database record manually curated predicted ncRNAs associated with In total, contains 645 experimentally verified entries, including 159 long non-coding (lncRNAs), 254 microRNAs (miRNAs), 39 circular (circRNAs), 5 other ncRNAs. The detailed information each entry includes ncRNA name, identifier, disease, reference, expression direction, tissue, species, so on. addition, also provides computationally stemness-associated for 33 TCGA cancers, which were prioritized using random walk restart (RWR) algorithm based on regulatory co-expression networks. total included 11 132 lncRNAs 972 miRNAs. Moreover, tools functional enrichment analysis, survival cell location interrogation summary, platform retrieve ncRNAs, may facilitate research offer potential targets treatment. Database URL: http://www.nidmarker-db.cn/ncStem/index.html.
Language: Английский
Citations
1