Biomedical Chromatography,
Journal Year:
2024,
Volume and Issue:
38(6)
Published: March 14, 2024
Qingxuan
Zhike
granules
(QXZKG),
a
traditional
Chinese
patent
medication,
has
shown
therapeutic
potential
against
acute
lung
injury
(ALI).
However,
the
precise
mechanism
underlying
its
lung-protective
effects
requires
further
investigation.
In
this
study,
integrated
network
pharmacology,
molecular
docking,
and
lipidomics
were
used
to
elucidate
QXZKG's
regulatory
effect
on
lipid
metabolism
in
lipopolysaccharide-induced
ALI.
Animal
experiments
conducted
substantiate
efficacy
of
QXZKG
reducing
pro-inflammatory
cytokines
mitigating
pulmonary
pathology.
Network
pharmacology
analysis
identified
145
active
compounds
that
directly
targeted
119
primary
targets
Gene
Ontology
function
emphasized
roles
mitogen-activated
protein
kinase
(MAPK)
cascade
as
crucial
biological
processes.
The
MAPK1
exhibited
promising
affinities
for
naringenin,
luteolin,
kaempferol.
Lipidomic
revealed
12
lipids
showed
significant
restoration
following
treatment
(p
<
0.05,
FC
>1.2
or
<0.83).
Specifically,
DG
38:4,
40:7,
PC
O-40:8,
TG
18:1_18:3_22:6,
PI
18:2_20:4,
FA
16:3,
20:3,
20:4,
22:5,
24:5
downregulated,
while
Cer
18:0;2O/24:0
SM
36:1;2O/34:5
upregulated
versus
model
groups.
This
study
enhances
our
understanding
QXZKG,
well
International Journal of Biological Macromolecules,
Journal Year:
2024,
Volume and Issue:
274, P. 133422 - 133422
Published: June 24, 2024
Small
extracellular
vesicles
derived
from
milk
(Milk-sEVs)
have
the
advantages
of
easy
availability,
low
cost,
toxicity,
and
inhibition
inflammation.
CD36
mediates
inflammation
stress
in
a
variety
disease
states.
The
purpose
this
study
was
to
investigate
role
Milk-sEVs
inhibiting
fibroblast
through
provide
reference
data
for
treatment
chronic
apical
periodontitis.
Journal of Applied Toxicology,
Journal Year:
2024,
Volume and Issue:
44(12), P. 1949 - 1961
Published: Aug. 14, 2024
Abstract
Pneumonia
is
a
serious
and
life‐threatening
lung
inflammation
with
high
morbidity
mortality.
Accumulating
evidence
has
suggested
that
esculin,
derivative
of
coumarin,
possesses
potent
anti‐inflammatory
effects.
This
study
designed
to
explore
the
pharma
role
underlying
mechanism
esculin
against
lipopolysaccharides
(LPS)‐induced
pneumonia.
TC‐1
cells
were
stimulated
by
LPS
mimic
inflammatory
injury
model
in
vitro
.
Cell
viability,
proliferation,
apoptosis
determined
using
MTT
assay,
5‐ethynyl‐2′‐deoxyuridine
flow
cytometry.
Interleukin‐1β
tumor
necrosis
factor
α
levels
analyzed
an
enzyme‐linked
immunosorbent
assay.
Reactive
oxygen
species
superoxide
dismutase
examined
special
assay
kits.
Macrophage
polarization
was
detected
Mitogen‐activated
protein
kinase
14
(MAPK14)
level
real‐time
quantitative
polymerase
chain
reaction.
MAPK14
ubiquitin‐specific
protease
7
(USP7)
western
blot
After
Ubibrowser
database
prediction,
interaction
between
USP7
verified
Co‐immunoprecipitation
The
biological
LPS‐challenged
ALI
mice
vivo
Here,
we
found
significantly
relieved
LPS‐induced
cell
proliferation
inhibition,
apoptosis,
response,
oxidative
stress,
M1‐type
macrophage
promotion.
expressions
enhanced
LPS‐treated
cells,
which
partly
abolished
treatment.
Overexpressing
attenuated
repression
on
LPS‐triggered
injury.
At
molecular
level,
interacted
maintained
its
stability
removing
ubiquitin.
Moreover,
repressed
progression
pneumonia
regulating
MAPK14.
Taken
together,
exposure
could
mitigate
targeting
USP7/MAPK14
axis,
providing
better
understanding
therapeutics
for
