Techniques for immobilizing enzymes to create durable and effective biocatalysts

Results in Chemistry, Journal Year: 2024, Volume and Issue: 7, P. 101486 - 101486

Published: Jan. 1, 2024

This comprehensive review focuses on strategies for creating efficient and robust immobilized biocatalysts. It explores various physicochemical approaches that hold promise enzyme stabilization enhancement of catalytic potential. One approach involves designing permeable scaffolds, emphasizing the importance support structure ability to fine-tune biocatalyst activity. Another pathway is establishing covalent bonds between enzymes surfaces, anchoring biocatalysts longevity sustained activity in industrial research applications. Polymers are also used modify formulation enzymes, enhancing stability providing a versatile platform biocatalytic reactions. These particularly valuable when within pre-established scaffolds. They enable researchers engineers utilize biocatalysis diverse applications, such as biopharmaceutical production sustainable chemical synthesis. Furthermore, these fixed-stabilized derivatives offer significant advancements reaction optimization reactor design. The reported enhancements remarkable, with performance gains ranging from 500 an impressive 20,000-fold compared diluted soluble enzymes. In summary, this highlights innovative developing presents exciting avenues industries seeking harness potential not only enhanced efficiency but contribute environmentally friendly processes.

Language: Английский

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Effects of duo-strain probiotics on growth, digestion, and gut health in broiler chickens

Veterinary and Animal Science, Journal Year: 2024, Volume and Issue: 24, P. 100343 - 100343

Published: March 2, 2024

The goal of this inquiry was to analyze the impact incorporating

Language: Английский

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Evaluation of mesoporous silica particles as a support for lipase immobilization in biodiesel production: Enhanced ethyl ester synthesis from algal oil

Results in Engineering, Journal Year: 2024, Volume and Issue: 22, P. 102138 - 102138

Published: April 16, 2024

The utilization of novel materials in the biofuel production domain has been regarded as a significant advancement catalyst development. Incorporating lipases biodiesel manufacturing is often viewed cost-restrictive phase since expenses associated with recovering these catalysts can render market expectations unfeasible. Within this investigation, mesoporous silica (MPS) particles were assessed supporting medium for immobilizing commercial lipase, subsequent to their ethyl ester synthesis. Initially, was achieved through co-precipitation technique at consistent pH and subsequently chosen an immobilization support enzyme. characterization biocatalyst produced performed using various analytical methods including X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), BET analysis. Novozym 435 lipase then immobilized within matrices, followed by transesterification process algal oil derived from Nannochloropsis. resulting samples demonstrated compliance standards, exhibiting conversion rate exceeding 94.8%. Additionally, properties such density (0.93 g.cm-3) viscosity (5.01 mm2.s-1) satisfied specifications mandated ASTM application. Experimental planning techniques showed that increasing loading temperatures between 38 48 °C resulted higher rates esters. This confirms effectiveness enzymatic generating primary fatty acid esters, even residual lipid feedstocks.

Language: Английский

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Comparative study of random and block SPEEK copolymers for high-temperature proton exchange membrane electrolysis

Colloids and Surfaces A Physicochemical and Engineering Aspects, Journal Year: 2024, Volume and Issue: 702, P. 134982 - 134982

Published: Aug. 5, 2024

Language: Английский

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Effects of potato extract on betalains, antioxidant activity, and sensory preference in buttermilk through fermentation with Lactobacillus acidophilus and Streptococcus salivarius

Future Foods, Journal Year: 2024, Volume and Issue: 9, P. 100357 - 100357

Published: May 1, 2024

Utilizing flavorings in drinks enhances aroma, preserves natural properties, and poses challenges production costs crop demands. Mitigating these is possible by employing waste products like potato skins. This investigation aims to explore the potential of dried mint-flavored stirred Buttermilk enriched with harvested water extract (HPE) for improved functionality acceptability. To achieve this objective, betalains, antioxidant, antimicrobial activities were examined at pH 4.5 5.5, along phenolic flavonoid content. Following that, HPE was incorporated different concentrations 3% (T1), 5% (T2), 7% (T3) assess stability physicochemical nutritional value, lactic acid bacteria (LAB) content during storage (for 0, 7, 14, 21 days 5°C). In comparison control group (0% HPE), inclusion remarkably (p<0.05) reduced pH, increased total acidity, LAB, vitamin C, antioxidant activity, sensory preference. Total LAB from 5.25 – 5.79 CFU/mL reached its peak 7%HPE addition, ranging between 5.76 6.28 CFU/mL. Vitamin C 4.78 mg/100 g 5.27 g, after days, it declined level group, 3.14 g. concentration resulted a more stable 21-day period. exhibited significant quantities betalains (478.42 mg/L), phenolics (156.44 mg/g), flavonoids (51.23 showcasing elevated levels Caffeic p-coumaric acid. The superior that 5.5 following period (332.21 253.22 mg/L, respectively). application led development functional drink boasting (90.02 ± 2.05 mg/g) (65.21 1.33 presence 7%HPE, as compared (78.91 2.55 59.32 1.46 mg/g, demonstrated notable DPPH inhibitory activity (69.31 1.1) (85.52 µL/mL). conclusion, addition can enhance properties buttermilk improve

Language: Английский

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Investigation of oil biodegradation using expanded zeolite infused with oil-consuming microorganisms

Environmental Advances, Journal Year: 2024, Volume and Issue: 16, P. 100551 - 100551

Published: May 24, 2024

The study examined the capabilities of expanded zeolites, which were modified with oil-consuming bacteria, in terms biosorption, biodegradation, and oil absorption. This investigation aimed to assess effectiveness zeolite as an absorbent for spills its potential eradicating such spills. research involved three different adsorbents: one without loaded laden bacteria. results indicated that zeolite, characterized by extensive surface region reduced density, provided conditions conducive development propagation microbes. After microbial establishment, absorption removal increased 60.5% 78.14%, respectively, contrast pre-enlargement stage. Additionally, microorganism functioning helped mitigate pollution interfacial tension amid water through generation surfactants. This, turn, facilitated separation residual from water.

Language: Английский

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The distribution of rennet activity between the cheese aging process and whey is not influenced by the association of enzymes with caseins

Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e32263 - e32263

Published: May 31, 2024

The division of rennet in cheesemaking is split between the curd and whey, influencing taste texture aged cheeses. Our study aimed to examine how raising protein concentration reconstituted skim milk (up 8.8 %) affects distribution calf activity (RA) curds produced through two methods: renting only renneting with glucono-δ-lactone (GDL) achieve slow acidification. into increased as rose, ranging from 8.6 % 29.1 without acidification, 6.5 19.4 when combined This increase seemed be related retention moisture protein. Surprisingly, residual RA whey (measured international clotting units, IMCU/mL) remained unaffected consistent initial IMCU/mL milk. suggests that not influenced by association enzymes caseins (CNs). Instead, it possible strength interactions CNs themselves plays a significant role. These findings could valuable for research focused on enhancing cheese aging process.

Language: Английский

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Optimizing glutamate production from microalgae extracts sourced from the Caspian Sea Basin: A cost-effective and sustainable approach with potential applications

Food Chemistry Advances, Journal Year: 2024, Volume and Issue: 4, P. 100742 - 100742

Published: June 1, 2024

The versatile free amino acid known as glutamate plays a key role in biological processes transmitter of signals between nerve cells, well the food industry flavor enhancer. Extensive endeavors have been undertaken to produce using various methods, often relying on costly and scarce natural resources. This study aims explore cost-effective abundant alternative for synthesis by utilizing microalgae extract sourced from Caspian Sea basin. To achieve this goal, submerged fermentation method was utilized. fermented extract, inoculated with P. putida, exhibited substantially higher concentrations compared uninoculated samples. Amongst seaweeds investigated, aqueous Chlorella highest Glutamate amount (3.1 ± 0.1 mg/g). Moreover, both fresh dehydrated extracts displayed noteworthy rise concentration soluble proteins during fermentation. It is worth mentioning that treated putida total levels overall phenolics flavonoids, suggesting promising improvement their biologically active combinations. Furthermore, functions alpha-amylase proteolytic enzymes experienced significant increments extracts. research highlights generating which has potential industry, offering numerous advantages.

Language: Английский

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Achieving optimal output of microplastic petroleum waste and improving energy and environmental indicators by optimizing the pyrolysis process and modifying the fuel composition

Fuel, Journal Year: 2024, Volume and Issue: 376, P. 132699 - 132699

Published: Aug. 10, 2024

Language: Английский

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Assessing the Efficacy of Poly-Ferric Sulfate and Polyaluminum Hydroxychloride in Remediating Partially Stabilized Landfill Effluent

Results in Engineering, Journal Year: 2024, Volume and Issue: unknown, P. 102996 - 102996

Published: Sept. 1, 2024

Language: Английский

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