Advances in Biomaterial‐Based Spinal Cord Injury Repair

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 32(13)

Published: Dec. 1, 2021

Abstract Spinal cord injury (SCI) often leads to the loss of motor and sensory functions is a major challenge in neurological clinical practice. Understanding pathophysiological changes inhibitory microenvironment crucial enable identification potential mechanisms for functional restoration provide guidance development efficient treatment repair strategies. To date, implantation specifically functionalized biomaterials lesion area has been shown help promote axon regeneration facilitate neuronal circuit generation by remolding SCI microenvironments. Moreover, structural spinal through transplantation naive tissue grafts from adult donors, artificial cord‐like developed engineering, 3D printing will open up new avenues treatment. This review focuses on dynamic microenvironments, repairs, strategies restoring structure function, experimental animal models, regenerative mechanisms, studies repair. The current status, recent advances, challenges, prospects scaffold‐based basic settings are summarized discussed, reference that guide future exploration

Language: Английский

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A conductive supramolecular hydrogel creates ideal endogenous niches to promote spinal cord injury repair

Bioactive Materials, Journal Year: 2021, Volume and Issue: 15, P. 103 - 119

Published: Dec. 23, 2021

The current effective method for treatment of spinal cord injury (SCI) is to reconstruct the biological microenvironment by filling injured cavity area and increasing neuronal differentiation neural stem cells (NSCs) repair SCI. However, characterized several challenges including irregular wounds, mechanical electrical mismatch material-tissue interface. In study, a unique facile agarose/gelatin/polypyrrole (Aga/Gel/PPy, AGP3) hydrogel with similar conductivity modulus as was developed altering concentration Aga PPy. gelation occurred through non-covalent interactions, physically crosslinked features made AGP3 hydrogels injectable. vitro cultures showed that exhibited excellent biocompatibility, promoted NSCs toward neurons whereas it inhibited over-proliferation astrocytes. in vivo implanted completely covered tissue defects reduced areas. studies further provided biocompatible promoting endogenous neurogenesis rather than glial fibrosis formation, resulting significant functional recovery. RNA sequencing analysis indicated significantly modulated expression neurogenesis-related genes intracellular Ca2+ signaling cascades. Overall, this supramolecular strategy produces can be used favorable biomaterials SCI imitating physiological properties cord.

Language: Английский

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Dynamic hyaluronic acid hydrogel with covalent linked gelatin as an anti-oxidative bioink for cartilage tissue engineering

Biofabrication, Journal Year: 2021, Volume and Issue: 14(1), P. 014107 - 014107

Published: Dec. 14, 2021

Abstract In the past decade, cartilage tissue engineering has arisen as a promising therapeutic option for degenerative joint diseases, such osteoarthritis, in hope of restoring structure and physiological functions. Hydrogels are biomaterials developing engineered scaffolds regeneration. However, hydrogel-delivered mesenchymal stem cells or chondrocytes could be exposed to elevated levels reactive oxygen species (ROS) inflammatory microenvironment after being implanted into injured joints, which may affect their phenotype normal functions thereby hinder regeneration efficacy. To attenuate ROS induced side effects, multifunctional hydrogel with an innate anti-oxidative ability was produced this study. The rapidly formed through dynamic covalent bond between phenylboronic acid grafted hyaluronic (HA-PBA) poly(vinyl alcohol) further stabilized secondary crosslinking acrylate moiety on HA-PBA free thiol group from thiolated gelatin. is cyto-compatible injectable can used bioink 3D bioprinting. viscoelastic properties hydrogels modulated precursor concentration. presence linkages contributed its shear-thinning property thus good printability hydrogel, resulting fabrication porous grid construct meniscus like scaffold at high structural fidelity. bioprinted promoted cell adhesion chondrogenic differentiation encapsulated rabbit adipose derived cells. Meanwhile, supported robust deposition extracellular matrix components, including glycosaminoglycans type II collagen, by embedded mouse vitro . Most importantly, protect downregulation cartilage-specific anabolic genes (ACAN COL2) upregulation catabolic gene (MMP13) incubation H 2 O Furthermore, intra-articular injection mice revealed adequate stability biocompatibility vivo These results demonstrate that novel generation constructs anti-ROS potentially enhance chronic microenvironment.

Language: Английский

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Engineering Complex Anisotropic Scaffolds beyond Simply Uniaxial Alignment for Tissue Engineering

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 32(15)

Published: Dec. 22, 2021

Abstract Anisotropic microarchitectures arising from an aligned organization of threadlike extracellular matrix (ECM) components or cells are ubiquitous in the human body, such as skeletal muscle, corneal stroma, and meniscus, for executing tissue‐specific physiological functions. It is widely recognized that tissue engineering, whereby growing implanted endogenous anisotropic scaffolds with geometrical resemblance to ECM targeted tissues, represents a promising solution structural functional restoration these tissues. However, remarkable challenges remain recapitulating complexities native tissues beyond simply uniaxial alignment. Through unremitting endeavors over past decade, some innovative bioengineering approaches developed tackle challenges. This review focuses on recent progress modular assembly 3D printing techniques exploited construct complex key highlight their accessibility features different types anisotropies, based understanding whole picture anisotropies alignment which geometrically divided into three categories. Finally, applications either vitro modeling vivo regeneration, explored.

Language: Английский

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The Role of Biomaterials in Peripheral Nerve and Spinal Cord Injury: A Review

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(3), P. 1244 - 1244

Published: Jan. 23, 2022

Peripheral nerve and spinal cord injuries are potentially devastating traumatic conditions with major consequences for patients' lives. Severe cases of these currently incurable. In both the peripheral nerves cord, disruption degeneration axons is main cause neurological deficits. Biomaterials offer experimental solutions to improve conditions. They can be engineered as scaffolds that mimic tissue extracellular matrix and, upon implantation, encourage axonal regeneration. Furthermore, biomaterial designed deliver therapeutic agents lesion site. This article presents principles recent advances in use biomaterials regeneration nervous system repair.

Language: Английский

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Scaffolds with anisotropic structure for neural tissue engineering

Engineered Regeneration, Journal Year: 2022, Volume and Issue: 3(2), P. 154 - 162

Published: April 26, 2022

Nervous system injuries remain a great challenge due to limited natural tissue regeneration capabilities. Neural engineering has been regarded as promising approach for repairing nerve defects, which utilizes external biomaterial scaffolds allow cells migrate the injury site and repair tissue. Particularly, with anisotropic structures biomimicking native extracellular matrix (ECM) can effectively guide neural orientation reconnection. Here, advancements of in field are presented. The fabrication strategies their effects vitro vivo highlighted. We also discuss challenges provide perspective this field.

Language: Английский

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Engineering multifunctional bioactive citrate-based biomaterials for tissue engineering

Bioactive Materials, Journal Year: 2022, Volume and Issue: 19, P. 511 - 537

Published: May 7, 2022

Developing bioactive biomaterials with highly controlled functions is crucial to enhancing their applications in regenerative medicine. Citrate-based polymers are the few polymer used biomedicine because of facile synthesis, controllable structure, biocompatibility, biomimetic viscoelastic mechanical behavior, and functional groups available for modification. In recent years, various multifunctional designs biomedical applications, including cardiovascular, orthopedic, muscle tissue, skin nerve spinal cord, bioimaging, drug or gene delivery based on citrate-based polymers, have been extensively studied, many them good clinical application potential. this review, we summarize progress design polymers. We also discuss further development tailored properties meet requirements applications.

Language: Английский

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Design of hydrogel-based scaffolds for in vitro three-dimensional human skin model reconstruction

Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 153, P. 13 - 37

Published: Sept. 30, 2022

Language: Английский

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Polydopamine-coated polycaprolactone/carbon nanotube fibrous scaffolds loaded with brain-derived neurotrophic factor for peripheral nerve regeneration

Biofabrication, Journal Year: 2022, Volume and Issue: 14(3), P. 035006 - 035006

Published: Feb. 22, 2022

Abstract Carbon nanotubes (CNTs) have attracted increasing attention in the field of peripheral nerve tissue engineering due to their unique structural and physical characteristics. In this study, a novel type aligned conductive scaffolds composed polycaprolactone (PCL) CNTs were fabricated via electrospinning. Utilizing mussel-inspired polydopamine (PDA) surface modification, brain-derived neurotrophic factor (BDNF) was loaded onto PCL/CNT fibrous obtain PCL/CNT-PDA-BDNF capable sustained release BDNF over 28 d. Schwann cells cultured on these scaffolds, effect regeneration vitro assessed by studying cell proliferation, morphology expressions myelination-related genes S100, P0 myelin basic protein. Furthermore, vivo investigated using 10 mm rat sciatic defect model. Both results indicate that effectively promote functional recovery. Therefore, great potential for restoration.

Language: Английский

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Collagen-based bioinks for regenerative medicine: Fabrication, application and prospective

Medicine in Novel Technology and Devices, Journal Year: 2023, Volume and Issue: 17, P. 100211 - 100211

Published: Feb. 1, 2023

In the field of regenerative medicine, importance 3D bioprinting is self-evident and nonnegligible. However, technology also requires bioink with excellent performance as support material to fabricate a multi-functional bioinspired scaffold. Collagen-based regarded an ideal ink for its biocompatibility, controllable printability cell loading property. It important breakthrough in medicine progress collagen-based bioink, which fabricates scaffolds different functions applied repair scenarios. This review summarizes applications classifies them soft tissue hard according target region. The region tissues include skin, cartilage, heart blood vessels, while femur, skull, teeth spine. When repairing tissue, requirements function are higher, mechanical properties must be further improved tissue. We summarize characteristics point out most that should considered scenarios, can provide reference preparation bioinks functions. Finally, we main challenges faced by prospect future research directions.

Language: Английский

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