Bioprinting of inorganic-biomaterial/neural-stem-cell constructs for multiple tissue regeneration and functional recovery DOI Creative Commons
Hongjian Zhang, Qin Chen, Zhe Shi

et al.

National Science Review, Journal Year: 2024, Volume and Issue: 11(4)

Published: Jan. 25, 2024

Tissue regeneration is a complicated process that relies on the coordinated effort of nervous, vascular and immune systems. While nervous system plays crucial role in tissue regeneration, current engineering approaches mainly focus restoring function injury-related cells, neglecting guidance provided by nerves. This has led to unsatisfactory therapeutic outcomes. Herein, we propose new generation engineered neural constructs from perspective induction, which offers versatile platform for promoting multiple regeneration. Specifically, consist inorganic biomaterials stem cells (NSCs), where endows NSCs with enhanced biological activities including proliferation differentiation. Through animal experiments, show effectiveness repairing central injuries recovery. More importantly, also stimulate osteogenesis, angiogenesis neuromuscular junction formation, thus bone skeletal muscle, exhibiting its performance. These findings suggest inorganic-biomaterial/NSC-based represents promising avenue inducing recovery varying tissues organs.

Language: Английский

Bone Microenvironment‐Mimetic Scaffolds with Hierarchical Microstructure for Enhanced Vascularization and Bone Regeneration DOI

Yujie Ha,

Xiaojun Ma, Shikai Li

et al.

Advanced Functional Materials, Journal Year: 2022, Volume and Issue: 32(20)

Published: Feb. 9, 2022

Abstract Microchannel networks within engineered 3D scaffold can allow nutrient exchange and rapid blood vessels formation. However, fabrication of a bone microenvironment‐mimicking with hierarchical micro/nanofibrous microchannel structures is still challenge. Herein, inspired by structural functional cues remodeling, networks‐enriched nanofibrous using printing thermally induced phase separation techniques, which facilitate cells migration nutrients transportation, developed. The customizable vascular‐like structure polycaprolactone the gelatin‐silica fabricated 3D‐printed sacrificial templates, while dimethyloxalylglycine (DMOG)‐loaded mesoporous silica nanoparticles (MSNs) located on surface forming peptide‐1 (BFP)‐loaded MSNs embedded in are implemented for sequential release DMOG BFP. cell experiments show that dual‐drug delivery (DBM/GP) promotes angiogenesis stimulating migration, tube formation, angiogenesis‐related genes/protein expression endothelial cells, osteogenesis promoting osteo‐related genes mineral deposition osteoblasts. Additionally, DBM/GP facilitates angiogenic activity osteoblasts activating phosphatidylinositol 3‐kinase/protein kinase B/hypoxia inducible factor‐1α pathway. Furthermore, enhanced vascularization regeneration demonstrated via subcutaneous skull defect models. Overall, this study reveals microenvironment‐mimetic provides promising strategy defects treatment.

Language: Английский

Citations

144
Collagen-Based Biomaterials for Tissue Engineering DOI
Yi-Yu Wang, Zhengke Wang, Dong Yan

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(3), P. 1132 - 1150

Published: Feb. 17, 2023

Collagen is commonly used as a regenerative biomaterial due to its excellent biocompatibility and wide distribution in tissues. Different kinds of hybridization or cross-links are favored offer improvements satisfy various needs biomedical applications. Previous reviews have been made introduce the sources structures collagen. In addition, biological mechanical properties collagen-based biomaterials, their modification application forms, interactions with host tissues pinpointed. However, there still no review about biomaterials for tissue engineering. Therefore, we aim summarize discuss progress materials regeneration applications this review. We focus on utilization bones, cartilages, skin, dental, neuron, cornea, urological hope these experiences outcomes can provide inspiration practical techniques future development related fields. Moreover, improving directions challenges proposed well.

Language: Английский

Citations

137
A biomimetic piezoelectric scaffold with sustained Mg2+ release promotes neurogenic and angiogenic differentiation for enhanced bone regeneration DOI Creative Commons
Liangyu Wang,

Yanyun Pang,

Yujing Tang

et al.

Bioactive Materials, Journal Year: 2022, Volume and Issue: 25, P. 399 - 414

Published: Nov. 29, 2022

Natural bone is a composite tissue made of organic and inorganic components, showing piezoelectricity. Whitlockite (WH), which natural magnesium-containing calcium phosphate, has attracted great attention in formation recently due to its unique piezoelectric property after sintering treatment sustained release magnesium ion (Mg2+). Herein, scaffold (denoted as PWH scaffold) composed WH (PWH) poly(ε-caprolactone) (PCL) was 3D printed meet the physiological demands for regeneration neuro-vascularized tissue, namely, providing endogenous electric field at defect site. The Mg2+ from scaffold, displaying multiple biological activities, thus exhibits strong synergistic effect with piezoelectricity on inhibiting osteoclast activation, promoting neurogenic, angiogenic, osteogenic differentiation marrow mesenchymal stromal cells (BMSCs) vitro. In rat calvarial model, this remarkably conducive efficient neo-bone rich neurogenic angiogenic expressions. Overall, study presents first example biomimetic vivo, offers new insights regenerative medicine.

Language: Английский

Citations

70
Advances in materials-based therapeutic strategies against osteoporosis DOI
Lei Chen,

Jing‐han Song,

Song Li

et al.

Biomaterials, Journal Year: 2023, Volume and Issue: 296, P. 122066 - 122066

Published: Feb. 20, 2023

Language: Английский

Citations

60
Polyhedron‐Like Biomaterials for Innervated and Vascularized Bone Regeneration DOI
Hongjian Zhang, Meng Zhang, Dong Zhai

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(42)

Published: July 12, 2023

Neural-vascular networks are densely distributed through periosteum, cortical bone, and cancellous which is of great significance for bone regeneration remodeling. Although significant progress has been made in tissue engineering, ineffective regeneration, delayed osteointegration still remains an issue due to the ignorance intrabony nerves blood vessels. Herein, inspired by space-filling polyhedra with open architectures, polyhedron-like scaffolds spatial topologies prepared via 3D-printing technology mimic meshwork structure bone. Benefiting from its topologies, greatly promoted osteogenic differentiation mesenchymal stem cells (BMSCs) activating PI3K-Akt signals, exhibiting satisfactory performance on angiogenesis neurogenesis. Computational fluid dynamic (CFD) simulation elucidates that have a relatively lower area-weighted average static pressure, beneficial osteogenesis. Furthermore, vivo experiments further demonstrate obviously promote formation osteointegration, as well inducing vascularization ingrowth nerves, leading innervated vascularized regeneration. Taken together, this work offers promising approach fabricating multifunctional without additional exogenous seeding growth factors, holds potential functional clinical translation.

Language: Английский

Citations

51
Biomimetic bone-periosteum scaffold for spatiotemporal regulated innervated bone regeneration and therapy of osteosarcoma DOI Creative Commons
Yan Xu, Chao Xu, Huan Song

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 15, 2024

The complexity of repairing large segment defects and eradicating residual tumor cell puts the osteosarcoma clinical management challenging. Current biomaterial design often overlooks crucial role precisely regulating innervation in bone regeneration. Here, we develop a Germanium Selenium (GeSe) co-doped polylactic acid (PLA) nanofiber membrane-coated tricalcium phosphate bioceramic scaffold (TCP-PLA/GeSe) that mimics bone-periosteum structure. This biomimetic offers dual functionality, combining piezoelectric photothermal conversion capabilities while remaining biodegradable. When subjected to ultrasound irradiation, US-electric stimulation TCP-PLA/GeSe enables spatiotemporal control neurogenic differentiation. feature supports early during formation, promoting differentiation Schwann cells (SCs) by increasing intracellular Ca

Language: Английский

Citations

16
Neuro-bone tissue engineering: Multiple potential translational strategies between nerve and bone DOI
Zhen Zhang,

Zhichao Hao,

Caihong Xian

et al.

Acta Biomaterialia, Journal Year: 2022, Volume and Issue: 153, P. 1 - 12

Published: Sept. 16, 2022

Language: Английский

Citations

48
Neuro–bone tissue engineering: emerging mechanisms, potential strategies, and current challenges DOI Creative Commons
Wenzhe Sun, Bing Ye, Siyue Chen

et al.

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Dec. 20, 2023

The skeleton is a highly innervated organ in which nerve fibers interact with various skeletal cells. Peripheral endings release neurogenic factors and sense signals, mediate bone metabolism pain. In recent years, tissue engineering has increasingly focused on the effects of nervous system regeneration. Simultaneous regeneration nerves through use materials or by enhancement endogenous repair signals been proven to promote functional Additionally, emerging information mechanisms interoception central regulation homeostasis provide an opportunity for advancing biomaterials. However, comprehensive reviews this topic are lacking. Therefore, review provides overview relationship between regeneration, focusing applications. We discuss novel regulatory explore innovative approaches based nerve-bone interactions Finally, challenges future prospects field briefly discussed.

Language: Английский

Citations

41
Bioprinted Scaffold Remodels the Neuromodulatory Microenvironment for Enhancing Bone Regeneration DOI
Shuting Guo, Chuanglong He

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(40)

Published: May 31, 2023

Abstract Herein, a 3D bioprinted scaffold is proposed, containing calcitonin gene‐related peptide (CGRP) and the β ‐adrenergic receptor blocker propranolol (PRN) as new method to achieve effective repair of bone defects. By leveraging neuromodulation mechanism regeneration, CGRP PRN loaded mesoporous silica nanoparticles are added into hybrid bio‐ink, which initially contains gelatin methacrylate, Poly (ethylene glycol) diacrylate marrow mesenchymal stem cells (BMSCs). Subsequently, optimized bio‐ink used for bioprinting create composite with pre‐designed micro‐nano hierarchical structure. The migration tube formation human umbilical vein endothelial (HUVECs) can be promoted by scaffold, beneficial capillary network during process. With release from secretion neuropeptides sensory nerves simulated. Meanwhile, inhibit binding process catecholamine receptor, co‐promoting osteogenic differentiation BMSCs silicon ions, will effectively enhance critical‐sized cranial defect in rat model. In conclusion, this study provides promising strategy understanding neuromodulatory mechanisms regeneration.

Language: Английский

Citations

37
Type H blood vessels in coupling angiogenesis‐osteogenesis and its application in bone tissue engineering DOI
Zhengyi Xu, Anjali P. Kusumbe, He Cai

et al.

Journal of Biomedical Materials Research Part B Applied Biomaterials, Journal Year: 2023, Volume and Issue: 111(7), P. 1434 - 1446

Published: March 7, 2023

Abstract One specific capillary subtype, termed type H vessel, has been found with unique functional characteristics in coupling angiogenesis osteogenesis. Researchers have fabricated a variety of tissue engineering scaffolds to enhance bone healing and regeneration through the accumulation vessels. However, only limited number reviews discussed strategies for vessel regulation. The object this review is summary current utilizes regulate vessels various signal pathways including Notch, PDGF‐BB, Slit3, HIF‐1α, VEGF signaling. Moreover, we give an insightful overview recent research progress about morphological, spatial age‐dependent blood Their role tying osteogenesis together via flow, cellular microenvironment, immune system nervous are also summarized. This article would provide insight into combination identify future perspectives vasculized research.

Language: Английский

Citations

33