Bioactive Materials, Journal Year: 2025, Volume and Issue: 48, P. 531 - 549
Published: March 3, 2025
Language: Английский
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(48)
Published: June 26, 2024
Abstract The growing concerns regarding cancer recurrence, unpredictable bone deficiencies, and postoperative bacterial infections subsequent to the surgical removal of tumors have highlighted need for multifaceted scaffolds that afford tumor therapy, effective vascularized reconstruction. However, challenging trilemma has emerged in realm balance between achieving appropriate mechanical strength, ensuring biocompatibility, optimizing a degradation rate aligns with bone‐regenerative rate. Considering these challenges, innovative theragenerative platform is developed by utilizing 3D printing‐based nanospikes first time. This comprises tissue‐specific nanospiked hydroxyapatite decorated magnesium (nMg) adhesive DNA (aDNA). incorporation nMg within polylactic acid (PLA) matrix confers photothermal capabilities helps modulate properties improve biocompatibility platform. Simultaneously, immobilized aDNA contributed enhancement healing. These 3D‐printed tissue‐adhesive platforms exhibit superior offer controlled degradability. Moreover, they enable eradication bacteria osteosarcoma through hyperthermia promote angiogenesis osteogenesis, both vitro vivo. groundbreaking approach poised pave way fabrication design novel implantable biomaterials integrate therapeutic regenerative functions.
Language: Английский
Citations
24
ACS Nano, Journal Year: 2024, Volume and Issue: 18(10), P. 7504 - 7520
Published: Feb. 27, 2024
The essential role of the neural network in enhancing bone regeneration has often been overlooked biomaterial design, leading to delayed or compromised healing. Engineered mesenchymal stem cells (MSCs)-derived exosomes are becoming increasingly recognized as potent cell-free agents for manipulating cellular behavior and improving therapeutic effectiveness. Herein, MSCs stimulated with nerve growth factor (NGF) regulate exosomal cargoes improve neuro-promotive potential facilitate innervated regeneration. In vitro cell experiments showed that NGF-stimulated MSCs-derived (N-Exos) obviously improved function neurotrophic effects cells, consequently, osteogenic osteo-reparative cells. Bioinformatic analysis by miRNA sequencing pathway enrichment revealed beneficial N-Exos may partly be ascribed NGF-elicited multicomponent miRNAs subsequent regulation activation MAPK PI3K-Akt signaling pathways. On this basis, were delivered on micropores 3D-printed hierarchical porous scaffold accomplish sustained release profile extended bioavailability. a rat model distal femoral defect, N-Exos-functionalized significantly induced neurovascular structure formation This study provided feasible strategy modulate functional acquire desirable potential. Furthermore, developed represent promising neurovascular-promotive reparative clinical translation.
Language: Английский
Citations
18
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: May 15, 2024
The complexity of repairing large segment defects and eradicating residual tumor cell puts the osteosarcoma clinical management challenging. Current biomaterial design often overlooks crucial role precisely regulating innervation in bone regeneration. Here, we develop a Germanium Selenium (GeSe) co-doped polylactic acid (PLA) nanofiber membrane-coated tricalcium phosphate bioceramic scaffold (TCP-PLA/GeSe) that mimics bone-periosteum structure. This biomimetic offers dual functionality, combining piezoelectric photothermal conversion capabilities while remaining biodegradable. When subjected to ultrasound irradiation, US-electric stimulation TCP-PLA/GeSe enables spatiotemporal control neurogenic differentiation. feature supports early during formation, promoting differentiation Schwann cells (SCs) by increasing intracellular Ca
Language: Английский
Citations
17
Aggregate, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 9, 2025
ABSTRACT The repair and functional reconstruction of bone defects resulting from trauma, surgical resection, degenerative diseases, congenital malformations are major clinical challenges. Bone tissue engineering has significant advantages in the treatment severe defects. Vascularized scaffolds gradually attracting attention development because their excellent biomimetic properties efficient efficiency. Three‐dimensional (3D) printing technology, which can be used to fabricate structures at different scales using a wide range materials, been production vascularized scaffolds. This review discusses research progress 3D for Angiogenesis‐osteogenesis coupling regeneration process is first introduced, followed by summary technologies, inks, bioactive factors Notably, this focuses on structural design strategies Finally, application medicine, as well challenges outlooks future development, described.
Language: Английский
Citations
2
Military Medical Research, Journal Year: 2025, Volume and Issue: 12(1)
Published: March 3, 2025
Abstract Bone tissue relies on the intricate interplay between blood vessels and nerve fibers, both are essential for many physiological pathological processes of skeletal system. Blood provide necessary oxygen nutrients to bone tissues, remove metabolic waste. Concomitantly, fibers precede during growth, promote vascularization, influence cells by secreting neurotransmitters stimulate osteogenesis. Despite critical roles components, current biomaterials generally focus enhancing intraosseous vessel repair, while often neglecting contribution nerves. Understanding distribution main functions in is crucial developing effective engineering. This review first explores anatomy highlighting their vital embryonic development, metabolism, repair. It covers innovative regeneration strategies directed at accelerating intrabony neurovascular system over past 10 years. The issues covered included material properties (stiffness, surface topography, pore structures, conductivity, piezoelectricity) acellular biological factors [neurotrophins, peptides, ribonucleic acids (RNAs), inorganic ions, exosomes]. Major challenges encountered neurovascularized materials clinical translation have also been highlighted. Furthermore, discusses future research directions potential developments aimed producing repair that more accurately mimic natural healing tissue. will serve as a valuable reference researchers clinicians novel into practice. By bridging gap experimental practical application, these advancements transform treatment defects significantly improve quality life patients with bone-related conditions.
Language: Английский
Citations
2
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(26)
Published: Feb. 22, 2024
Abstract Bioceramics are vital for treating bone defects, and bioactive glasses (exemplified by 45S5) calcium phosphate ceramics (CaPs, exemplified tricalcium [β‐TCP]) extensively explored. β‐TCP exhibits superior biocompatibility, degradability, osteoconductive properties than 45S5; however, it lacks bioactivity, such as mineralization capability. To harness the synergies of both, four 3D printing bioceramic scaffolds: 45S5, 70% 45S5 + 30% TCP, manufactured. Furthermore, investigation elucidates correlation between their in situ capabilities intracellular oscillations within macrophages determines how they impact macrophage phenotypic transitions. Notably, during degradation, there is an initial rise followed a decline ion concentration, which results macrophages. In TCP group, early release ions promotes M1 polarization. Subsequently, rapid causes decrease extracellular ions, thus accelerating transition to M2 facilitating repair. The present study reveals novel mechanism through bioceramics modulate polarization, offers new insights into foreign body response presents perspective on expeditious progression toward tissue
Language: Английский
Citations
14
Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15
Published: Jan. 1, 2024
Bone defect disease seriously endangers human health and affects beauty function. In the past five years, three dimension (3D) printed radially graded triply periodic minimal surface (TPMS) porous scaffold has become a new solution for repairing bone defects. This review discusses 3D printing technologies applications TPMS scaffolds. To this end, microstructural effects of scaffolds on regeneration were reviewed structural characteristics TPMS, which can promote regeneration, introduced. Finally, challenges prospects using to treat defects presented. is expected stimulate interest tissue engineers in provide reliable clinical treatment personalised
Language: Английский
Citations
10
National Science Review, Journal Year: 2024, Volume and Issue: 11(4)
Published: Jan. 25, 2024
Tissue regeneration is a complicated process that relies on the coordinated effort of nervous, vascular and immune systems. While nervous system plays crucial role in tissue regeneration, current engineering approaches mainly focus restoring function injury-related cells, neglecting guidance provided by nerves. This has led to unsatisfactory therapeutic outcomes. Herein, we propose new generation engineered neural constructs from perspective induction, which offers versatile platform for promoting multiple regeneration. Specifically, consist inorganic biomaterials stem cells (NSCs), where endows NSCs with enhanced biological activities including proliferation differentiation. Through animal experiments, show effectiveness repairing central injuries recovery. More importantly, also stimulate osteogenesis, angiogenesis neuromuscular junction formation, thus bone skeletal muscle, exhibiting its performance. These findings suggest inorganic-biomaterial/NSC-based represents promising avenue inducing recovery varying tissues organs.
Language: Английский
Citations
9
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 488, P. 151136 - 151136
Published: April 9, 2024
Language: Английский
Citations
9
Biofabrication, Journal Year: 2024, Volume and Issue: 17(1), P. 012005 - 012005
Published: Nov. 8, 2024
Abstract Artificial bone graft stands out for avoiding limited source of autograft as well susceptibility to infection allograft, which makes it a current research hotspot in the field defect repair. However, traditional design and manufacturing method cannot fabricate scaffold that mimics complicated bone-like shape with interconnected porous structure multiple properties akin human natural bone. Additive manufacturing, can achieve implant’s tailored external contour controllable fabrication internal microporous structure, is able form almost any designed via layer-by-layer process. As additive promising building artificial scaffold, only combining excellent structural appropriate process produce ideal biological mechanical properties. In this article, we sum up analyze state art methods realize shape/properties collaborative intelligent manufacturing. Scaffold be mainly classified into based on unit cells whole while basic 3D bioprinting are recommended suitable fabrication. The challenges future perspectives manufactured also discussed.
Language: Английский
Citations
9