Extracellular vesicle-derived silk fibroin nanoparticles loaded with MFGE8 accelerate skin ulcer healing by targeting the vascular endothelial cells DOI Creative Commons
Liwen Luo, Hong Yu Zhang, Shiyu Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: Nov. 29, 2023

Reduced supplies of oxygen and nutrients caused by vascular injury lead to difficult-to-heal pressure ulcers (PU) in clinical practice. Rapid repair the skin wound is key resolution this challenge, but measures are still limited. We described beneficial effects extracellular vesicle-derived silk fibroin nanoparticles (NPs) loaded with milk fat globule EGF factor 8 (MFGE8) on accelerating blood vessel PU healing targeting CD13 endothelial cells (VECs).CD13, specific protein NGR, MFGE8, an inhibitor ferroptosis, were detected VECs tissues. Then, NPs synthesized via fibroin, MFGE8-coated (NPs@MFGE8) assembled loading purified MFGE8 produced Chinese hamster ovary cells. Lentivirus was used over-express obtained MFGE8-engineered vesicles (EVs-MFGE8) secreted these VECs. The inhibitory effect EVs-MFGE8 or NPs@MFGE8 ferroptosis vitro. NGR peptide cross-linked into NGR-NPs@MFGE8. Collagen synthesize fibroin/collagen hydrogel. After being NGR-NPs@MFGE8, hydrogel sustained-release carrier investigate vivo.MFGE8 decreased, increased Similar inhibiting could inhibit mitochondrial autophagy-induced Compared hydrogels NPs@MFGE8, NGR-NPs@MFGE8 consistently released VECs, thereby autophagy hypoxia effectively rats.The great significance use as a dressing tissues, preventing formation promoting healing.

Language: Английский

Intervertebral disc degeneration and osteoarthritis: a common molecular disease spectrum DOI
Noah Fine, Starlee Lively, Cheryle A. Séguin

et al.

Nature Reviews Rheumatology, Journal Year: 2023, Volume and Issue: 19(3), P. 136 - 152

Published: Jan. 26, 2023

Language: Английский

Citations

104
The role of oxidative stress in intervertebral disc degeneration: Mechanisms and therapeutic implications DOI Creative Commons
Xiang‐Long Chen, Anran Zhang, Kangcheng Zhao

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 98, P. 102323 - 102323

Published: May 9, 2024

Oxidative stress is one of the main driving mechanisms intervertebral disc degeneration(IDD). has been associated with inflammation in disc, cellular senescence, autophagy, and epigenetics cells. It above pathological are closely linked through common hub reactive oxygen species(ROS), promote each other process degeneration development disease. This reveals important role oxidative IDD, importance great potential IDD therapy targeting stress. The efficacy traditional unstable or cannot be maintained. In recent years, due to rise materials science, many bioactive functional have applied treatment combination drugs, satisfactory achieved. At present, research review antioxidant not complete. Based on existing studies, mechanism were summarized this paper, strategies based emerging reviewed.

Language: Английский

Citations

25
Lactic acid promotes nucleus pulposus cell senescence and corresponding intervertebral disc degeneration via interacting with Akt DOI Open Access
Yuyao Zhang, Libangxi Liu, Yuhan Qi

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Jan. 12, 2024

Language: Английский

Citations

22
Hydrogel-exosome system in tissue engineering: A promising therapeutic strategy DOI Creative Commons

Ming‐Hui Fan,

Jin-Kui Pi,

Chen‐Yu Zou

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 38, P. 1 - 30

Published: April 23, 2024

Characterized by their pivotal roles in cell-to-cell communication, cell proliferation, and immune regulation during tissue repair, exosomes have emerged as a promising avenue for "cell-free therapy" clinical applications. Hydrogels, possessing commendable biocompatibility, degradability, adjustability, physical properties akin to biological tissues, also found extensive utility engineering regenerative repair. The synergistic combination of hydrogels holds the potential not only enhance efficiency but collaboratively advance repair process. This review has summarized advancements made over past decade research hydrogel-exosome systems regenerating various tissues including skin, bone, cartilage, nerves tendons, with focus on methods encapsulating releasing within hydrogels. It critically examined gaps limitations current research, whilst proposed future directions applications this innovative approach.

Language: Английский

Citations

22
Application of mesenchymal stem cell-derived exosomes from different sources in intervertebral disc degeneration DOI Creative Commons

Yuanliang Xia,

Ruohan Yang,

Yulin Hou

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 7, 2022

Intervertebral disc degeneration (IVDD) is a main cause of lower back pain, leading to psychological and economic burdens patients. Physical therapy only delays pain in patients but cannot eliminate the IVDD. Surgery required when patient tolerate or has severe neurological symptoms. Although surgical resection IVD decompression laminae eliminates diseased segment, it damages adjacent normal IVD. There also risk re-protrusion after removal. Cell played crucial role development regenerative medicine. transplantation promotes regeneration degenerative tissue. However, owing lack vascular structure IVD, sufficient nutrients be provided for transplanted mesenchymal stem cells (MSCs). In addition, dead release harmful substances that aggravate Extracellular vesicles (EVs) have been extensively studied as an emerging therapeutic approach. EVs generated by paracrine MSCs retain potential serve carriers deliver their contents target regulate cell activity. Owing double-layered membrane structure, low immunogenicity no immune rejection. Therefore, are considered modality they limited mass production loading rates. this review, advantages introduced, application MSC-EVs IVDD discussed. The current limitations future applications described.

Language: Английский

Citations

39
Enhanced Mitochondrial Targeting and Inhibition of Pyroptosis with Multifunctional Metallopolyphenol Nanoparticles in Intervertebral Disc Degeneration DOI
Hao Zhou, Qiuping Qian, Qizhu Chen

et al.

Small, Journal Year: 2023, Volume and Issue: 20(13)

Published: Nov. 12, 2023

Intervertebral disc degeneration (IVDD) is a significant contributor to low back pain, characterized by excessive reactive oxygen species generation and inflammation-induced pyroptosis. Unfortunately, there are currently no specific molecules or materials available effectively delay IVDD. This study develops multifunctional full name of PG@Cu nanoparticle network (PG@Cu). A designed pentapeptide, bonded on nanoparticles via Schiff base bond, imparts multifunctionality the metal polyphenol particles (PG@Cu-FP). PG@Cu-FP exhibits enhanced escape from lysosomal capture, enabling efficient targeting mitochondria scavenge excess species. The scavenging activity against originates polyphenol-based structures within nanoparticles. Furthermore, Pyroptosis blocked inhibiting Gasdermin mediated pore formation membrane rupture. successfully reduces activation nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 inflammasome protein (Gasdermin D, GSDMD) oligomerization, leading reduced expression Nod-like receptors. multifaceted approach demonstrates higher efficiency in Pyroptosis. Experimental results confirm that preserves height, retains water content, tissue structure. These findings highlight potential improving IVDD provide novel insights for future research treatments.

Language: Английский

Citations

28
A new strategy for intervertebral disc regeneration: The synergistic potential of mesenchymal stem cells and their extracellular vesicles with hydrogel scaffolds DOI Open Access
Yan Zhao,

Huaize Dong,

Qiuqiu Xia

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 172, P. 116238 - 116238

Published: Feb. 3, 2024

Intervertebral disc degeneration (IDD) is a disease that severely affects spinal health and prevalent worldwide. Mesenchymal stem cells (MSCs) their derived extracellular vesicles (EVs) have regenerative potential emerged as promising therapeutic tools for treating degenerative discs. However, challenges such the harsh microenvironment of degenerated intervertebral discs EVs' limited stability efficacy hindered clinical application. In recent years, hydrogels attracted much attention in field IDD therapy because they can mimic physiologic provide solution by providing suitable growth environment MSCs EVs. This review introduced biological properties EVs, summarized research on application EVs IDD, current trial studies also explored mechanism action addition, plenty elaborated different classified tissue engineering, synergistic effect promoting regeneration, wide IDD. Finally, problems still faced hydrogel-loaded treatment are summarized, solutions proposed. paper outlines effects combination with aims to theoretical references future related studies.

Language: Английский

Citations

11
Chondrocyte-targeted exosome-mediated delivery of Nrf2 alleviates cartilaginous endplate degeneration by modulating mitochondrial fission DOI Creative Commons
Zhidi Lin, Guangyu Xu, Xiao Lu

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 24, 2024

Abstract Background Cartilaginous endplate (CEP) degeneration, which is an important contributor to intervertebral disc degeneration (IVDD), characterized by chondrocyte death. Accumulating evidence has revealed that dynamin-related protein 1 (Drp1)-mediated mitochondrial fission and dysfunction lead apoptosis during CEP IVDD. Exosomes are promising agents for the treatment of many diseases, including osteoporosis, osteosarcoma, osteoarthritis Despite their major success in drug delivery, full potential exosomes remains untapped. Materials methods In vitro vivo models were established using lipopolysaccharide (LPS). We designed genetically engineered (CAP-Nrf2-Exos) expressing chondrocyte-affinity peptide (CAP) on surface carrying antioxidant transcription factor nuclear E2-related 2 (Nrf2). The affinity between CAP-Nrf2-Exos was evaluated internalization assays imaging assays. qRT‒PCR, Western blotting immunofluorescence performed examine expression level Nrf2 subcellular localization Drp1. Mitochondrial function measured JC-1 probe MitoSOX Red. morphology visualized MitoTracker staining transmission electron microscopy (TEM). After subendplate injection exosomes, degree IVDD validated radiologically histologically. Results found cargo delivery efficiency after packaging increased modification. facilitated chondrocyte-targeted activated endogenous defence system cells. inhibited Drp1 S616 phosphorylation translocation, thereby preventing fragmentation dysfunction. LPS-induced cell alleviated CAP-Nrf2-Exo treatment. a rat model successfully attenuated exhibited better repair capacity than natural exosomes. Conclusion Collectively, our findings showed exosome-mediated effective strategy treating degeneration. Graphic abstract delivered into cells inhibiting Drp1-mediated

Language: Английский

Citations

11
Innovating intervertebral disc degeneration therapy: Harnessing the power of extracellular vesicles DOI Creative Commons
Shanfeng Chen,

Yiming Dou,

Yiming Zhang

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 44 - 55

Published: Jan. 1, 2025

Language: Английский

Citations

1
Autophagy activation alleviates annulus fibrosus degeneration via the miR-2355-5p/mTOR pathway DOI Creative Commons
Zilin Yu,

Chunyang Fan,

Yubo Mao

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1