Innovative Approach: MRI-Guided Fabrication of a Biomimetic Intervertebral Disc Scaffold DOI Creative Commons

Yuchen Ye,

Chen Shao, Yi Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

Abstract Study Design：A biomimetic intervertebral disc (IVD) scaffold was fabricated through the integration of magnetic resonance imaging (MRI) and 3D printing technology, subsequently underwent in vitro experimentation. Objective：This study aimed to create a new IVD using MRI grayscale image analysis GE-DLP technology mimic natural structure for improved biomechanical performance cell compatibility, potentially providing treatment option regeneration. Summary Background Data: The is critical component spine, its damage or degeneration can lead severe back neck pain. Current treatments provide symptomatic relief but do not address structural damage. Tissue engineering offers promising alternative, with bio-scaffolds being key element Methods: In this study, we constructed model from scans healthy volunteer processed images distinguish between tissue types. Exposure times were adjusted based on values, applied fabricate single integrated process bicomponent polymer network (BCN) hydrogel laden nucleus pulposus stem cells (NPMSCs). The microstructure porosity analyzed scanning electron microscopy (SEM), elastic modulus across radial distribution tested via nanoindentation. evaluated finite (FEA). For biocompatibility assessment, cytoskeleton staining utilized observe morphology, viability Calcein/PI staining. Results: exhibited gradient changes pore size, consistent ultrastructure characteristics IVD. FEA indicated that scaffold's response terms displacement, strain, stress closely resembled an actual IVD, particularly during simulations left rotation lateral bending. SEM revealed pores varying sizes different regions scaffold, which crucial adhesion growth. also demonstrated high biocompatibility, survival rates maintained at level over seven-day culture period. Conclusion: research, have successfully engineered novel excellent static integrity by integrating technology. Level Evidence: N/A

Language: Английский

Innovating intervertebral disc degeneration therapy: Harnessing the power of extracellular vesicles DOI Creative Commons
Shanfeng Chen,

Yiming Dou,

Yiming Zhang

et al.

Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 50, P. 44 - 55

Published: Jan. 1, 2025

Language: Английский

Citations

1
ISSLS Prize in Clinical Science 2025: Cartilage End Plate Defects Precede and Initiate Bony End Plate Defects and Disc Degeneration– An ‘Integrated Total End Plate Score’ Identify Preclinical Discs at Risk for Degeneration DOI

Shanmuganathan Rajasekaran,

Pushpa Bhari Thippeswamy,

Gnanaprakash Gurusamy

et al.

European Spine Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

Language: Английский

Citations

0
Injectable hydrogel microspheres promoting inflammation modulation and nucleus pulposus-like differentiation for intervertebral disc regeneration DOI
Yuhang Chen, Zhuoran Yang, Zhangrong Cheng

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 599 - 614

Published: Feb. 15, 2025

Language: Английский

Citations

0
Effects of Hydrogels on Mesenchymal Stem/Stromal Cells Paracrine Activity and Extracellular Vesicles Production DOI Creative Commons

Oscar Fabian Garcia‐Aponte,

Simon Kahlenberg,

Dimitrios Kouroupis

et al.

Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(3)

Published: March 1, 2025

ABSTRACT Mesenchymal stem/stromal cells (MSCs) are a valuable source of paracrine factors, as they have remarkable secretory capacity, and there is sizeable knowledge base to develop industrial clinical production protocols. Promising cell‐free approaches for tissue regeneration immunomodulation driving research towards secretome applications, among which extracellular vesicles (EVs) steadily gaining attention. However, the manufacturing application EVs limited by insufficient yields, gaps, low standardization. Facing these limitations, hydrogels represent versatile three‐dimensional (3D) culture platform that can incorporate matrix (ECM) components mimic natural stem cell environment in vitro; via niche‐mimicking properties, regulate MSCs’ morphology, adhesion, proliferation, differentiation secretion capacities. impact hydrogel's architectural, biochemical biomechanical properties on remains poorly understood, field still its infancy interdependency parameters compromises comparability studies. Therefore, this review summarizes discusses reported effects hydrogel encapsulation MSC‐EVs. Considering cell‐material interactions overall activity MSCs, we identify persistent challenges from standardization process control, outline future paths research, such synergic use bioreactors enhance MSC‐EV generation.

Language: Английский

Citations

0
Application Trends and Strategies of Hydrogel Delivery Systems in Intervertebral Disc Degeneration: A Bibliometric Review DOI Creative Commons
Junwu Wang, Yu Zhang, Yilong Huang

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 28, P. 101251 - 101251

Published: Sept. 14, 2024

Language: Английский

Citations

1
Small Extracellular Vesicles by Nucleus Pulposus Cells Maintain Niche and Cell Homeostasis via Receptor Shuffling and Metabolic Enzyme Supplements DOI Creative Commons

Ankita Samanta,

Mi‐Jeong Yoo, Jin Koh

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 14, 2024

Background: Small extracellular vesicles (sEV) are a conserved mean of communication across the domains life and lately gained more interest in mammalian non-cancerous work as non-cellular, biological therapeutic with encouraging results recent studies chronic degenerative diseases. The nucleus pulposus (NP) is avascular aneural center an intervertebral disc (IVD), home to unique niche conditions affected IVD degeneration. We investigated proteome autologous mesenchymal stem cell (MSC) sEVs for their potential contribute tissue homeostasis this niche. Methods: aimed identify characteristics NP via mass spectrometric functional enrichment analysis. explored profile generated by parent lines from bovine coccygeal adipose tissue, focus on cells, using adult fetal donors. compared these findings published sEV databases MSC data. Findings: propose several mechanisms associated sEVs: 1. Membrane receptor trafficking modify signal responses promoting homeostasis. 2. Cell proteasome delivery; 3. Redox energy metabolic enzymes delivery. 4. Immunomodulation beyond association serum protein corona. Conclusion: signature cells similar previously data, yet supplementing anaerobic metabolism redox balance while contributing maintenance microniche.

Language: Английский

Citations

1
AQP3-liposome@GelMA promotes overloaded-induced degenerated disc regeneration via IBSP/ITG αVβ3/AKT pathway DOI
Junxian Hu, Yibo Zhu, Xiaoxiao Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 292, P. 139238 - 139238

Published: Dec. 27, 2024

Language: Английский

Citations

0
Orthobiologic Management Options for Degenerative Disc Disease DOI Creative Commons

Cezar Augusto Alves de Oliveira,

Bernardo Scaldini Oliveira,

Rafael Theodoro

et al.

Bioengineering, Journal Year: 2024, Volume and Issue: 11(6), P. 591 - 591

Published: June 10, 2024

Degenerative disc disease (DDD) is a pervasive condition that limits quality of life and burdens economies worldwide. Conventional pharmacological treatments primarily aimed at slowing the progression degeneration have demonstrated limited long-term efficacy often do not address underlying causes disease. On other hand, orthobiologics are regenerative agents derived from patient's own tissue represent promising emerging therapy for degenerative This review comprehensively outlines pathophysiology DDD, highlighting inadequacies existing therapies detailing potential orthobiologic approaches. It explores advanced tools such as platelet-rich plasma mesenchymal stem cells, providing historical overview their development within medicine, foundational in vitro studies to preclinical animal models. Moreover, manuscript delves into clinical trials assess effectiveness these managing DDD. While current evidence promising, it remains insufficient routine adoption due limitations study designs. The emphasizes need further research optimize consistent effective outcomes, potentially revolutionizing management DDD offering renewed hope patients.

Language: Английский

Citations

0
miRNA-124 loaded extracellular vesicles encapsulated within hydrogel matrices for combating chemotherapy-induced neurodegeneration DOI
Pankaj Pal, Monika Sharma, Sukesh Kumar Gupta

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 734, P. 150778 - 150778

Published: Oct. 1, 2024

Language: Английский

Citations

0
Facile fabrication of nano-bioactive glass functionalized blended hydrogel with nucleus pulposus-derived MSCs to improve regeneration potential in treatment of disc degeneration by in vivo rat model DOI
Chong Bian, Guangnan Chen,

Xiang-yang Cheng

et al.

Nanomedicine Nanotechnology Biology and Medicine, Journal Year: 2024, Volume and Issue: unknown, P. 102790 - 102790

Published: Oct. 1, 2024

Language: Английский

Citations

0