Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
unknown, P. 101701 - 101701
Published: March 1, 2025
Three-dimensional
(3D)
bioprinting
of
hydrogels
allows
embedded
cells
to
be
patterned
and
hosted
in
an
extracellular
matrix
(ECM)-mimicking
environment.
This
method
shows
great
promise
for
the
engineering
complex
tissues
on
account
facile
spatial
control
over
materials
within
printed
constructs.
Hydrogels,
which
represent
extensively
explored
employed
biomaterials
3D
bioprinting,
are
characterized
by
both
their
high
water
content
swelling
behavior.
Post-printing
inevitably
alters
geometrical
mechanical
properties
features,
thus
causing
a
deviation
from
original
design
affecting
cellular
function
tissue
structure.
Despite
substantial
effort
being
dedicated
development
non-swelling
hydrogels,
application
encapsulation
living
is
yet
realized,
owing
limitations
imposed
often
tedious
material
syntheses
network
structures.
Herein,
we
describe
new
type
hydrogel
based
fully
cold
fish
gelatin
(cfGel-Hydrogel)
consisting
only
single
formed
via
thiol-ene
"click"
chemistry.
We
show
that
such
cfGel-Hydrogels
enable
patterning
shape-retaining
mechanically
robust
matrix.
These
negligible
(<2
%)
under
physiologically
relevant
conditions
(simulated
37
°C
PBS
buffer),
while
also
able
withstand
large
cyclic
deformations
(80
%
compressive
strain)
dissipating
around
40
loading
energy.
Human
dermal
fibroblast
(HDF)-laden
could
fabricated
extrusion-based
printing,
allowing
vitro
culturing
constructs,
offering
opportunities
hydrogel-based
applications
regenerative
medicine.
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(2), P. 564 - 589
Published: Jan. 4, 2024
As
a
biodegradable
and
biocompatible
protein
derived
from
collagen,
gelatin
has
been
extensively
exploited
as
fundamental
component
of
biological
scaffolds
drug
delivery
systems
for
precise
medicine.
The
easily
engineered
holds
great
promise
in
formulating
various
to
protect
enhance
the
efficacy
drugs
improving
safety
effectiveness
numerous
pharmaceuticals.
remarkable
biocompatibility
adjustable
mechanical
properties
permit
construction
active
3D
accelerate
regeneration
injured
tissues
organs.
In
this
Review,
we
delve
into
diverse
strategies
fabricating
functionalizing
gelatin-based
structures,
which
are
applicable
gene
well
tissue
engineering.
We
emphasized
advantages
derivatives,
including
methacryloyl
gelatin,
polyethylene
glycol-modified
thiolated
alendronate-modified
gelatin.
These
derivatives
exhibit
excellent
physicochemical
properties,
allowing
fabrication
tailor-made
structures
biomedical
applications.
Additionally,
explored
latest
developments
modulation
their
by
combining
additive
materials
manufacturing
platforms,
outlining
design
multifunctional
micro-,
nano-,
macrostructures.
While
discussing
current
limitations,
also
addressed
challenges
that
need
be
overcome
clinical
translation,
high
costs,
limited
application
scenarios,
potential
immunogenicity.
This
Review
provides
insight
how
structural
chemical
engineering
can
leveraged
pave
way
significant
advancements
applications
improvement
patient
outcomes.
Journal of Nanobiotechnology,
Journal Year:
2024,
Volume and Issue:
22(1)
Published: Aug. 27, 2024
With
the
accelerated
aging
tendency,
osteoarthritis
(OA)
has
become
an
intractable
global
public
health
challenge.
Stem
cells
and
their
derivative
exosome
(Exo)
have
shown
great
potential
in
OA
treatment.
Research
this
area
tends
to
develop
functional
microcarriers
for
stem
cell
Exo
delivery
improve
therapeutic
effect.
Herein,
we
a
novel
system
of
Exo-encapsulated
cell-recruitment
hydrogel
from
liquid
nitrogen-assisted
microfluidic
electrospray
Benefited
advanced
droplet
generation
capability
microfluidics
mild
cryogelation
procedure,
resultant
particles
show
uniform
size
dispersion
excellent
biocompatibility.
Moreover,
acryloylated
recruitment
peptides
SKPPGTSS
are
directly
crosslinked
within
by
ultraviolet
irradiation,
thus
simplifying
peptide
coupling
process
preventing
its
premature
release.
The
SKPPGTSS-modified
can
recruit
endogenous
promote
cartilage
repair
released
further
enhances
performance
through
synergistic
effects.
These
features
suggest
that
proposed
microcarrier
is
promising
candidate
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(4), P. 2243 - 2260
Published: March 25, 2024
Cartilage
repair
has
been
a
significant
challenge
in
orthopedics
that
not
yet
fully
resolved.
Due
to
the
absence
of
blood
vessels
and
almost
cell-free
nature
mature
cartilage
tissue,
limited
ability
resulted
socioeconomic
pressures.
Polysaccharide
materials
have
recently
widely
used
for
tissue
due
their
excellent
cell
loading,
biocompatibility,
chemical
modifiability.
They
also
provide
suitable
microenvironment
regeneration.
In
this
Review,
we
summarize
techniques
clinically
repair,
focusing
on
polysaccharides,
polysaccharides
differences
between
these
other
materials.
addition,
engineering
strategies
an
outlook
developing
next-generation
regeneration
from
polysaccharides.
This
Review
will
theoretical
guidance
polysaccharide-based
with
clinical
applications
