Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: April 2, 2024
The
tissues
or
organs
derived
decellularized
extracellular
matrix
carry
immunogenicity
and
the
risk
of
pathogen
transmission,
resulting
in
limited
therapeutic
effects.
cell
dECM
cultured
vitro
can
address
these
potential
risks,
but
its
impact
on
wound
remodeling
is
still
unclear.
This
study
aimed
to
explore
role
(dECM)
extracted
from
adipose
stem
cells
(ADSCs)
skin
regeneration.
Methods:
ADSCs
were
human
tissue.
Then
we
cultivated
adipose-derived
ADSC-dECM
for
freeze-drying.
Western
blot
(WB),
enzyme-linked
immunosorbent
assay
(ELISA)
mass
spectrometry
(MS)
conducted
analyzed
main
protein
components
ADSC-dECM.
counting
(CCK-8)
scratch
used
effects
different
concentrations
proliferation
migration
keratinocytes
(HaCaT),
umbilical
vein
endothelia
(HUVEC)
fibroblasts
(HFB),
respectively.
Moreover,
designed
a
novel
ADSC-dECM-CMC
patch
which
carboxymethylcellulose
(CMC)
load
with
ADSC-dECM;
further
investigated
effect
mouse
full
thickness
model.
Results:
was
obtained
after
decellularization
ADSCs.
blot,
ELISA
results
showed
that
contained
various
bioactive
molecules,
including
collagen,
elastin,
laminin,
growth
factors.
CCK-8
treatment
could
significantly
promote
HaCaT,
cells,
fibroblasts,
To
evaluate
healing
vivo
,
developed
transplanted
it
into
full-thickness
And
found
accelerated
closure
time.
Further
histology
immunohistochemistry
indicated
tissue
regeneration,
as
confirmed
via
enhanced
angiogenesis
high
proliferative
activity.
Conclusion:
In
this
study,
containing
multiple
molecules
exhibiting
good
biocompatibility
reconstruction
provides
new
approach
use
engineering.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: May 31, 2024
Myocardial
infarction
(MI)
stands
as
a
prominent
contributor
to
global
cardiovascular
disease
(CVD)
mortality
rates.
Acute
MI
(AMI)
can
result
in
the
loss
of
large
number
cardiomyocytes
(CMs),
which
adult
heart
struggles
replenish
due
its
limited
regenerative
capacity.
Consequently,
this
deficit
CMs
often
precipitates
severe
complications
such
failure
(HF),
with
whole
transplantation
remaining
sole
definitive
treatment
option,
albeit
constrained
by
inherent
limitations.
In
response
these
challenges,
integration
bio-functional
materials
within
cardiac
tissue
engineering
has
emerged
groundbreaking
approach
significant
potential
for
replacement.
Bioengineering
strategies
entail
fortifying
or
substituting
biological
tissues
through
orchestrated
interplay
cells,
methodologies,
and
innovative
materials.
Biomaterial
scaffolds,
crucial
paradigm,
provide
essential
microenvironment
conducive
assembly
functional
encapsulating
contracting
cells.
Indeed,
field
witnessed
remarkable
strides,
largely
owing
application
biomaterial
scaffolds.
However,
complexities
persist,
necessitating
further
exploration
innovation.
This
review
delves
into
pivotal
role
scaffolds
engineering,
shedding
light
on
their
utilization,
challenges
encountered,
promising
avenues
future
advancement.
By
critically
examining
current
landscape,
we
aim
catalyze
progress
toward
more
effective
solutions
regeneration
ultimately,
improved
outcomes
patients
grappling
ailments.
Cell Reports Methods,
Journal Year:
2024,
Volume and Issue:
4(6), P. 100779 - 100779
Published: May 16, 2024
Organoids,
self-organizing
three-dimensional
(3D)
structures
derived
from
stem
cells,
offer
unique
advantages
for
studying
organ
development,
modeling
diseases,
and
screening
potential
therapeutics.
However,
their
translational
ability
to
mimic
complex
in
vivo
functions
are
often
hindered
by
the
lack
of
an
integrated
vascular
network.
To
address
this
critical
limitation,
bioengineering
strategies
rapidly
advancing
enable
efficient
vascularization
organoids.
These
methods
encompass
co-culturing
organoids
with
various
cell
types,
lineage-specific
organoids,
co-differentiating
cells
into
organ-specific
lineages,
using
organoid-on-a-chip
technology
integrate
perfusable
vasculature
within
3D
bioprinting
also
create
This
review
explores
field
organoid
vascularization,
examining
biological
principles
that
inform
approaches.
Additionally,
envisions
how
converging
disciplines
biology,
biomaterials,
advanced
fabrication
technologies
will
propel
creation
increasingly
sophisticated
models,
ultimately
accelerating
biomedical
discoveries
innovations.
Bioactive Materials,
Journal Year:
2024,
Volume and Issue:
40, P. 597 - 623
Published: Aug. 15, 2024
Tissue
engineering
technology
has
advanced
rapidly
in
recent
years,
offering
opportunities
to
construct
biologically
active
tissues
or
organ
substitutes
repair
even
enhance
the
functions
of
diseased
and
organs.
Tissue-engineered
scaffolds
rebuild
extracellular
microenvironment
by
mimicking
matrix.
Fibrin-based
possess
numerous
advantages,
including
hemostasis,
high
biocompatibility,
good
degradability.
Fibrin
provide
an
initial
matrix
that
facilitates
cell
migration,
differentiation,
proliferation,
adhesion,
also
play
a
critical
role
cell-matrix
interactions.
are
now
widely
recognized
as
key
component
tissue
engineering,
where
they
can
facilitate
defect
repair.
This
review
introduces
properties
fibrin,
its
composition,
structure,
biology.
In
addition,
modification
cross-linking
modes
fibrin
discussed,
along
with
various
forms
commonly
used
engineering.
We
describe
biofunctionalization
fibrin.
provides
detailed
overview
use
applications
skin,
bone,
nervous
tissues,
novel
insights
into
future
research
directions
for
clinical
treatment.
Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials,
Journal Year:
2024,
Volume and Issue:
151, P. 106391 - 106391
Bioengineering,
Journal Year:
2025,
Volume and Issue:
12(2), P. 136 - 136
Published: Jan. 30, 2025
Whole-organ
decellularization
generates
scaffolds
containing
native
extracellular
matrix
(ECM)
components
with
preserved
tissue
microarchitecture,
providing
a
promising
advancement
in
engineering
and
regenerative
medicine.
Decellularization
retains
the
ECM
integrity
which
is
important
for
supporting
cell
attachment,
growth,
differentiation,
biological
function.
Although
there
are
consensus
guidelines
to
standardize
processes
characterization,
no
specific
criteria
or
standards
regarding
sterility
biosafety
have
been
established
so
far.
This
regulatory
gap
safety,
sterilization,
regulation
has
hampered
clinical
translation
of
decellularized
scaffolds.
In
this
review,
we
identify
essential
safe
use
products
from
both
human
animal
sources.
These
include
efficacy,
levels
chemical
residue,
preservation
composition
physical
characteristics,
aseptic
processing
assure
sterility.
Furthermore,
explore
key
considerations
advancing
into
practice,
focusing
on
frameworks
safety
requirements.
Addressing
these
challenges
crucial
minimizing
risks
adverse
reactions
infection
transmission,
thereby
accelerating
adoption
tissue-engineered
products.
review
aims
provide
foundation
establishing
robust
guidelines,
effective
integration
medicine
applications.
Materials Today Chemistry,
Journal Year:
2024,
Volume and Issue:
37, P. 102016 - 102016
Published: April 1, 2024
Tissue-engineered
constructs
can
replicate
the
structural
and
physiological
properties
of
natural
tissues.
The
be
designed
to
address
transplantation
issues
affected
by
shortage
donor
tissues
organs.
One
major
concerns
in
tissue
engineering
is
design
development
structures
that
improve
interaction
between
materials
cells
provide
an
ideal
platform
for
form
functional
tissue.
Several
contributing
factors
need
considered
fabricate
constructs,
including
biomaterials,
biological,
topographical,
biophysical,
morphological
either
alone
or
combination.
Here,
we
review
application,
advancement,
future
directions
these
essential
designing
developing
regeneration.
In
particular,
focus
on
original
approaches
tools
construct
parameters
engineering.
Materials Today Bio,
Journal Year:
2024,
Volume and Issue:
26, P. 101032 - 101032
Published: March 18, 2024
The
distinctive
three-dimensional
architecture,
biological
functionality,
minimal
immunogenicity,
and
inherent
biodegradability
of
small
intestinal
submucosa
extracellular
matrix
materials
have
attracted
considerable
interest
found
wide-ranging
applications
in
the
domain
tissue
regeneration
engineering.
This
article
presents
a
comprehensive
examination
structure
role
submucosa,
delving
into
diverse
preparation
techniques
classifications.
Additionally,
it
proposes
approaches
for
evaluating
modifying
SIS
scaffolds.
Moreover,
advancements
skin,
bone,
heart
valves,
blood
vessels,
bladder,
uterus,
urethra
are
thoroughly
explored,
accompanied
by
their
respective
future
prospects.
Consequently,
this
review
enhances
our
understanding
organ
repair
keeps
researchers
up-to-date
with
latest
research
area.
Carbohydrate Polymers,
Journal Year:
2024,
Volume and Issue:
348, P. 122845 - 122845
Published: Oct. 17, 2024
Polysaccharide
hydrogels,
which
can
mimic
the
natural
extracellular
matrix
and
possess
appealing
physicochemical
biological
characteristics,
have
emerged
as
significant
bioinks
for
3D
bioprinting.
They
are
highly
promising
applications
in
tissue
engineering
regenerative
medicine
because
of
their
ability
to
enhance
cell
adhesion,
proliferation,
differentiation
a
manner
akin
cellular
environment.
This
review
comprehensively
examines
fabrication
methods,
polysaccharide
hydrogel-driven
bioprinting,
underscoring
its
potential
engineering,
drug
delivery,
medicine.
To
contribute
pertinent
knowledge
future
research
this
field,
critically
key
aspects,
including
chemistry
carbohydrates,
manufacturing
techniques,
formulation
bioinks,
characterization
polysaccharide-based
hydrogels.
Furthermore,
explores
primary
advancements
3D-printed
encompassing
delivery
systems
with
controlled
release
kinetics
targeted
therapy,
along
tissue-engineered
constructs
bone,
cartilage,
skin,
vascular
regeneration.
The
use
these
bioprinted
hydrogels
innovative
fields,
disease
modeling
screening,
is
also
addressed.
Despite
notable
progress,
challenges,
modulating
properties
polysaccharides,
enhancing
bioink
printability
mechanical
properties,
achieving
long-term
vivo
stability,
been
highlighted.
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2024,
Volume and Issue:
12
Published: Jan. 23, 2024
Chronic
wound
management
is
an
intractable
medical
and
social
problem,
affecting
the
health
of
millions
worldwide.
Decellularized
extracellular
matrix
(dECM)-based
materials
possess
remarkable
biological
properties
for
tissue
regeneration,
which
have
been
used
as
commercial
products
skin
regeneration
in
clinics.
However,
complex
external
environment
longer
chronic
wound-healing
process
hinder
application
pure
dECM
materials.
dECM-based
composite
are
constructed
to
promote
healing
different
wounds,
showing
noteworthy
functions,
such
anti-microbial
activity
suitable
degradability.
Moreover,
fabrication
technologies
designing
dressings
with
various
forms
expanded
This
review
provides
a
summary
recent
building
materials,
highlighting
advances
molded
hydrogels,
electrospun
fibers,
bio-printed
scaffolds
managing
wounds.
The
associated
challenges
prospects
clinical
finally
discussed.
