CRISPR/Cas12a dual-mode biosensor for Staphylococcus aureus detection via enzyme-free isothermal amplification DOI

Hongmin Gao,

Hehua Zhang,

Xue Qi

et al.

Talanta, Journal Year: 2024, Volume and Issue: 282, P. 127013 - 127013

Published: Oct. 10, 2024

Language: Английский

Molecularly imprinted polymers-aptamer electrochemical sensor based on dual recognition strategy for high sensitivity detection of chloramphenicol DOI

Lingjun Geng,

Jiashuai Sun,

Mengyue Liu

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 437, P. 137933 - 137933

Published: Nov. 7, 2023

Language: Английский

Citations

88

Sensitive and selective DNA detecting electrochemical sensor via double cleaving CRISPR Cas12a and dual polymerization on hyperbranched rolling circle amplification DOI
Juneseok You, Hyunjun Park,

Hakbeom Lee

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 224, P. 115078 - 115078

Published: Jan. 10, 2023

Language: Английский

Citations

42

Capacitive immunosensing at gold nanoparticle-decorated reduced graphene oxide electrodes fabricated by one-step laser nanostructuration DOI Creative Commons
Danilo Echeverri,

Enric Calucho,

José Marrugo-Ramírez

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 252, P. 116142 - 116142

Published: Feb. 20, 2024

Nanostructured electrochemical biosensors have ushered in a new era of diagnostic precision, offering enhanced sensitivity and specificity for clinical biomarker detection. Among them, capacitive biosensing enables ultrasensitive label-free detection multiple molecular targets. However, the complexity cost associated with conventional fabrication methods nanostructured platforms hinder widespread adoption these devices. This study introduces biosensor that leverages laser-engraved reduced graphene oxide (rGO) electrodes decorated gold nanoparticles (AuNPs). The involves laser-scribed GO-Au

Language: Английский

Citations

22

Advances in Electrochemical Biosensors Based on Nanomaterials for Protein Biomarker Detection in Saliva DOI Creative Commons
Tao Dong, Nuno Pires,

Zhaochu Yang

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(6)

Published: Dec. 30, 2022

Abstract The focus on precise medicine enhances the need for timely diagnosis and frequent monitoring of chronic diseases. Moreover, recent pandemic severe acute respiratory syndrome coronavirus 2 poses a great demand rapid detection surveillance viral infections. protein biomarkers antigens in saliva allows identification diseases or disease changes scenarios where when test response at point care is mandated. While traditional methods testing fail to provide desired fast results, electrochemical biosensors based nanomaterials hold perfect characteristics point‐of‐care settings. advances sensors salivary are critically reviewed this work, with emphasis role boost biosensor analytical performance increase reliability human samples. Furthermore, work identifies critical factors further modernization nanomaterial‐based sensors, envisaging development implementation next‐generation sample‐in‐answer‐out systems.

Language: Английский

Citations

62

Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications DOI
Zijie Zhang, Payel Sen, Bal Ram Adhikari

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(50)

Published: Sept. 30, 2022

Nucleic acids are remarkable molecules. In addition to Watson-Crick base pairing, the different structural motifs of these molecules can bind non-nucleic acid targets or catalyze chemical reactions. Additionally, nucleic easily modified with functional groups. These properties make acids, particularly DNA, ideally suited for use in electrochemical biosensors, both as biorecognition elements and redox reporter probes. this Minireview, we will review historical evolution probes biosensors. We then specific examples nucleic-acid-based biosensors that have been evaluated clinical areas infectious disease, cancer, cardiovascular health.

Language: Английский

Citations

54

Paper-based colorimetric detection of COVID-19 using aptasenor based on biomimetic peroxidase like activity of ChF/ZnO/CNT nano-hybrid DOI

Mostafa Vafabakhsh,

Mehdi Dadmehr, Sakineh Kazemi Noureini

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2023, Volume and Issue: 301, P. 122980 - 122980

Published: June 4, 2023

Language: Английский

Citations

37

Label-Free Electrochemical Methods for Disease Detection DOI Creative Commons

Kira L. Rahn,

Umesha Peramune,

Tianyi Zhang

et al.

Annual Review of Analytical Chemistry, Journal Year: 2023, Volume and Issue: 16(1), P. 49 - 69

Published: Feb. 28, 2023

Label-free electrochemical biosensing leverages the advantages of label-free techniques, low cost, and fewer user steps, with sensitivity portability analysis. In this review, we identify four mechanisms: ( a) blocking electrode surface, b) allowing greater access to c) changing intercalation or electrostatic affinity a redox probe biorecognition unit, d) modulating ion electron transport properties due conformational surface charge changes. Each mechanism is described, recent advancements are summarized, relative disadvantages techniques discussed. Furthermore, two avenues for gaining further diagnostic information from biosensors, through multiplex analysis incorporating machine learning, examined.

Language: Английский

Citations

25

Aptamer-based assembly systems for SARS-CoV-2 detection and therapeutics DOI

Yuhang Dong,

Jingping Wang,

Ling Chen

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(13), P. 6830 - 6859

Published: Jan. 1, 2024

This review presents the recent progress on aptamers that have been explored for SARS-CoV-2 detection and therapeutics, wherein construction principles characteristics of aptamer-based assembly systems are systematically summarized.

Language: Английский

Citations

15

CRISPR-Driven Biosensors: A New Frontier in Rapid and Accurate Disease Detection DOI

ZahraSadat Razavi,

M. Soltani, Mohammad Souri

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Sept. 17, 2024

This comprehensive review delves into the advancements and challenges in biosensing, with a strong emphasis on transformative potential of CRISPR technology for early rapid detection infectious diseases. It underscores versatility CRISPR/Cas systems, highlighting their ability to detect both nucleic acids non-nucleic acid targets, seamless integration isothermal amplification techniques. The provides thorough examination latest developments CRISPR-based biosensors, detailing unique properties such as high specificity programmability, which make them particularly effective detecting disease-associated acids. While focuses due its critical role diagnosing diseases, it also explores broader applications thereby acknowledging technology's potential. Additionally, identifies existing challenges, need improved signal real-world applicability, offers future perspectives aimed at overcoming these hurdles. ultimate goal is advance development highly sensitive specific biosensors that can be used widely improving human health, point-of-care settings resource-limited environments.

Language: Английский

Citations

14

Triple-helix as a target converter for trace pesticide detection based on CRISPR/Cas12a-based ECL biosensor DOI
Wen Li, Yan Li, Lidan Zhao

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 409, P. 135599 - 135599

Published: March 4, 2024

Language: Английский

Citations

13