Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 137145 - 137145
Published: Dec. 1, 2024
Language: Английский
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 495, P. 153315 - 153315
Published: June 21, 2024
Language: Английский
Citations
6
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
With the increasing incidence of thyroid cancer worldwide and demand for surgery, risk parathyroid injury is also increasing, which will lead to postoperative hypoparathyroidism (HP) hypocalcemia. In order improve quality life patients after there an urgent need develop a novel platform that can identify gland immediately during surgery. The promotes increase blood calcium concentration by secreting hormone (PTH). Therefore, in this study, we used sensitive surface-enhanced Raman spectroscopy (SERS) technique rapid detection PTH, while near-infrared dye IR808 was resonance with incident excitation wavelength 785 nm achieve surface enhanced (SERRS) effect provide higher signal. signal up 200-fold compared nonresonant sulfhydryl molecules. addition, aptamer-antibody mode low Gibbs free energy (ΔG) dissociation constant (Kd), using sandwich method, specific recognition PTH. By optimizing incubation temperature, aptamer dosage, particle concentration, other key factors, optimal conditions system were obtained. sensitivity PTH as high 9.75 pg/mL at 5 min time. work, achieved extension from standard products rats clinically practical samples. Finally, eluate samples obtained actual clinical operations lateral flow biosensor (LFB), chemiluminescence microparticle immunoassay (CMIA), SERRS, respectively. CMIA results reference standard, SERRS assay 94% specificity 100%. This study provides new ideas methods refined development improving safety patients, promoting intraoperative diagnosis treatment.
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137605 - 137605
Published: March 1, 2025
Language: Английский
Citations
0
ACS Sensors, Journal Year: 2025, Volume and Issue: unknown
Published: April 9, 2025
Nanomaterials-integrated CRISPR/Cas systems have rapidly emerged as powerful next-generation platforms for optical biosensing. These integrated harness the precision of CRISPR/Cas-mediated nucleic acid detection while leveraging unique properties nanomaterials to achieve enhanced sensitivity and expanded analytical capabilities, thereby broadening their diagnostic potential. By incorporating a diverse range nanomaterials, these effectively expand toolbox detection, offering adaptable solutions tailored various challenges. This review provides comprehensive overview successfully into CRISPR/Cas-based sensing systems. It examines multiple modalities, including fluorescence, electrochemiluminescence, colorimetry, surface-enhanced Raman spectroscopy, highlighting how facilitate signal amplification, enable multiplexing, support development point-of-care applications. Additionally, practical applications in critical fields such healthcare diagnostics environmental monitoring are showcased. While offer considerable advantages, several real-world challenges complexity assay workflows, impact cost, regulatory hurdles must be addressed before widespread implementation can achieved. identifying obstacles proposing strategic solutions, we aim pave way continued advancement adoption nanomaterial-integrated biosensing technologies.
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137867 - 137867
Published: April 1, 2025
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 414, P. 135966 - 135966
Published: May 13, 2024
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11280 - 11289
Published: July 2, 2024
Here, ultrasmall SiO
Language: Английский
Citations
2
Food Chemistry, Journal Year: 2024, Volume and Issue: 468, P. 142468 - 142468
Published: Dec. 12, 2024
Language: Английский
Citations
2
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(45), P. 18178 - 18186
Published: Oct. 29, 2024
Per- and polyfluoroalkyl substances (PFASs) are a class of persistent micropollutants. Due to their chemical stability bioaccumulation, concentrations PFASs in environmental media, even at ultratrace levels, pose significant health risks. However, currently reported detection methods lack an effective signal amplification strategy, the sensitivity is limited, which can not meet requirements detection. Herein, groundbreaking aptamer-recognition-driven nucleic acid strategy was developed significantly amplify perfluorooctanoic (PFOA). Furthermore, step pulse (SP) used instead cyclic voltammetry (CV) as electrochemical excitation method modulate low electrochemiluminescence (ECL) triggering potential poly [9,9-bis (3′-(N, N-dimethylamino) propyl) −2,7-fluorene]-alt-2,7-(9,9-dioctylfluorene)] (PFN) nanoparticles (NPs) so that strong +0.80 V emitted without any exogenous coreactants. PFN NPs coupled rolling circle amplification-assisted PAM-free CRISPR/Cas12a system construct ultrasensitive ECL aptasensor for PFOA limit 1.97 × 10–15 M. This integrated advantages no coreactants, trigger potential, provided monitoring trace real water sample.
Language: Английский
Citations
2
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 118058 - 118058
Published: Nov. 1, 2024
Language: Английский
Citations
1