Bacillus subtilis biofilm formation and social interactions

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 19(9), P. 600 - 614

Published: April 6, 2021

Language: Английский

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Exopolysaccharides from agriculturally important microorganisms: Conferring soil nutrient status and plant health

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 262, P. 129954 - 129954

Published: Feb. 8, 2024

Language: Английский

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Enhanced surface colonisation and competition during bacterial adaptation to a fungus

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 27, 2024

Abstract Bacterial-fungal interactions influence microbial community performance of most ecosystems and elicit specific behaviours, including stimulating specialised metabolite production. Here, we use a co-culture experimental evolution approach to investigate bacterial adaptation the presence fungus, using simple model bacterial-fungal encompassing bacterium Bacillus subtilis fungus Aspergillus niger . We find in one evolving population that B. was selected for enhanced production lipopeptide surfactin accelerated surface spreading ability, leading inhibition fungal expansion acidification environment. These phenotypes were explained by mutations DegS-DegU two-component system. In surfactin, hyphae exhibited bulging cells with delocalised secretory vesicles possibly provoking an RlmA-dependent cell wall stress. Thus, our results indicate selects increased production, which inhibits growth facilitates competitive success bacterium.

Language: Английский

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Translational challenges and opportunities in biofilm science: a BRIEF for the future

npj Biofilms and Microbiomes, Journal Year: 2022, Volume and Issue: 8(1)

Published: Aug. 29, 2022

Abstract Biofilms are increasingly recognised as a critical global issue in multitude of industries impacting health, food and water security, marine sector, industrial processes resulting estimated economic cost $5 trillion USD annually. A major barrier to the translation biofilm science is gap between practices academic research across biofilms field. Therefore, there an urgent need for notice react industrially relevant issues achieve transferable outputs. Regulatory frameworks necessarily bridge gaps different players, but require clear, science-driven non-biased underpinning successfully translate research. Here we introduce 2-dimensional framework, termed Biofilm Research-Industrial Engagement Framework (BRIEF) classifying existing technologies according their level scientific insight, including understanding underlying system, utility accounting current practices. We evidence BRIEF with three case studies healthcare, & agriculture, wastewater sectors highlighting multifaceted around effective Based on these studies, some advisory guidelines enhance translational impact future

Language: Английский

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The biology and chemistry of a mutualism between a soil bacterium and a mycorrhizal fungus

Current Biology, Journal Year: 2024, Volume and Issue: 34(21), P. 4934 - 4950.e8

Published: Oct. 7, 2024

Highlights•B. velezensis colonizes the entire mycelial network more efficiently than roots•Bacillus uses fungal hyphae as highways for soil invasion and to colonize new plant•The lipopeptide surfactin plays key roles in chemical ecology of interaction•The microbial partnership enhances systemic resistance tomato against BotrytisSummaryArbuscular mycorrhizal (AM) fungi (e.g., Rhizophagus species) recruit specific bacterial species their hyphosphere. However, interplay mutual benefit this intricate have not been investigated yet, especially it involves bacteria known strong producers antifungal compounds such Bacillus velezensis. Here, we show that soil-dwelling B. migrates along hyphal AM fungus R. irregularis, forming biofilms inducing cytoplasmic flow contributes host plant root colonization by bacterium. During hyphosphere colonization, irregularis modulates biosynthesis specialized metabolites ensure stable coexistence a mechanism ward off mycoparasitic bacteria. These benefits are extended into tripartite context via provision enhanced protection through induction resistance.Graphical abstract

Language: Английский

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Bacterial-fungal interactions under agricultural settings: from physical to chemical interactions

Stress Biology, Journal Year: 2022, Volume and Issue: 2(1)

Published: June 1, 2022

Abstract Bacteria and fungi are dominant members of environmental microbiomes. Various bacterial-fungal interactions (BFIs) their mutual regulation important factors for ecosystem functioning health. Such can be highly dynamic, often require spatiotemporally resolved assessments to understand the interplay which ranges from antagonism mutualism. Many these still poorly understood, especially in terms underlying chemical molecular interplay, is crucial inter-kingdom communication interference. BFIs relevant under agricultural settings; they determinative crop Advancing our knowledge related mechanisms underpinning between bacteria will provide an extended basis biological control pests pathogens agriculture. Moreover, it facilitate a better understanding complex microbial community networks that commonly occur nature. This allow us determine assembly different conditions pave way constructing synthetic communities various biotechnological applications. Here, we summarize current advances field with emphasis on

Language: Английский

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Pulcherrimin protects Bacillus subtilis against oxidative stress during biofilm development

npj Biofilms and Microbiomes, Journal Year: 2023, Volume and Issue: 9(1)

Published: July 19, 2023

Pulcherrimin is an iron-binding reddish pigment produced by various bacterial and yeast species. In the soil bacterium Bacillus subtilis, this synthesized intracellularly as colorless pulcherriminic acid using two molecules of tRNA-charged leucine substrate; are then secreted bind to ferric iron extracellularly form red-colored pulcherrimin. The biological importance pulcherrimin not well understood. A previous study showed that secretion caused depletion in surroundings growth arrest on cells located at edge a B. subtilis colony biofilm. study, we identified primarily under biofilm conditions provides protection against oxidative stress. We presented molecular evidence how lowers level reactive oxygen species (ROS) alleviates stress DNA damage ROS accumulation mature also performed global transcriptome profiling identify differentially expressed genes pulcherrimin-deficient mutant compared with wild type, further characterized regulation related homeostasis, response (DDR), response. Based our findings, propose important antioxidant modulates development.

Language: Английский

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Role of Bacillus subtilis exopolymeric genes in modulating rhizosphere microbiome assembly

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: May 14, 2024

Abstract Background Bacillus subtilis is well known for promoting plant growth and reducing abiotic biotic stresses. Mutant gene-defective models can be created to understand important traits associated with rhizosphere fitness. This study aimed analyze the role of exopolymeric genes in modulating tomato microbiome assembly under a gradient soil diversities using B. wild-type strain UD1022 its corresponding mutant eps−TasA , which defective exopolysaccharide ( EPS ) TasA protein production. Results qPCR revealed that eps−TasA− has diminished capacity colonize roots soils diluted microbial diversity. The analysis bacterial β-diversity significant differences fungal community structures following inoculation either or strains. Verrucomicrobiota Patescibacteria, Nitrospirota phyla were more enriched than inoculation. Co-occurrence when was inoculated tomato, exhibited lower level modularity, fewer nodes, communities compared . Conclusion advances our understanding genes, are not only root colonization but also play shaping assembly. Future research should concentrate on specific genetic their implications colonization, coupled modulation. These efforts will crucial optimizing PGPR-based approaches agriculture.

Language: Английский

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Let’s Get Physical: Bacterial-Fungal Interactions and Their Consequences in Agriculture and Health

Journal of Fungi, Journal Year: 2020, Volume and Issue: 6(4), P. 243 - 243

Published: Oct. 23, 2020

Fungi serve as a biological scaffold for bacterial attachment. In some specialized interactions, the bacteria will invade fungal host, which in turn provides protection and nutrients bacteria. Mechanisms of physical interactions between fungi have been studied both clinical agricultural settings, discussed this review. that are part these dynamic can altered growth development well changes microbial fitness it pertains to antibiotic resistance, nutrient acquisition, dispersal. Consequences not just limited respective microorganisms, but also major impacts health humans plants alike. Examining mechanisms behind provide us with an understanding multi-kingdom community processes allow therapeutic approaches disease ecological settings.

Language: Английский

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Phage co-transport with hyphal-riding bacteria fuels bacterial invasion in a water-unsaturated microbial model system

The ISME Journal, Journal Year: 2021, Volume and Issue: 16(5), P. 1275 - 1283

Published: Dec. 13, 2021

Abstract Nonmotile microorganisms often enter new habitats by co-transport with motile microorganisms. Here, we report that also lytic phages can hyphal-riding bacteria and facilitate bacterial colonization of a habitat. This is comparable to the concept biological invasions in macroecology. In analogy invasion frameworks plant animal ecology, tailored spatially organized, water-unsaturated model microcosms using hyphae Pythium ultimum as paths flagellated soil-bacterium Pseudomonas putida KT2440 carrier for Escherichia virus T4. P. efficiently dispersed along dispatched T4 across air gaps transporting ≈0.6 bacteria−1. No displacement was observed absence bacteria. If E. coli occupied habitat, fueled fitness invading KT2440, while phage led poor followed extinction. Our data emphasize importance hyphal transport associated regulating composition microbial populations systems. As such seems analogous macroecological processes, hyphosphere systems co-transported could be useful models testing hypotheses ecology.

Language: Английский

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