Silver nanoparticles synthesized from Pseudomonas aeruginosa pyoverdine: Antibiofilm and antivirulence agents

Biofilm, Journal Year: 2024, Volume and Issue: 7, P. 100192 - 100192

Published: March 15, 2024

The increasing incidence of antimicrobial resistance exhibited by biofilm-forming microbial pathogens has been recognized as one the major issues in healthcare sector. In present study, nanomaterial-based controlling biofilm and virulence properties considered an alternative approach. Pyoverdine (PVD) isolated from Pseudomonas aeruginosa was utilized a biological corona to synthesize silver nanoparticles (AgNPs), which will be helpful targeted action due recognition pathogenic membrane. Synthesized PVD-AgNPs were spherical irregular, with average size value 251.87 ± 21.8 nm zeta potential −36.51 0.69 mV. MIC towards P. aeruginosa, Listeria monocytogenes, Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans standard host-mimicking media observed decreasing order multi-fold, such growth > sputum synthetic human urine saliva. Both initial stage well-established biofilms these have effectively inhibited eradicated PVD-AgNPs. increase susceptibility tetracycline, PVD, amphotericin B established mature mono- mixed-species S. aureus C. albicans. Additionally, attenuate several properties, inhibition protease activity, motility, PVD pyocyanin production aeruginosa. gene expression virulence-associated genes validates its phenotypic effects.

Language: Английский

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Investigating Biofilms: Advanced Methods for Comprehending Microbial Behavior and Antibiotic Resistance

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(4), P. 133 - 133

Published: March 29, 2024

Biofilms, which consist of microorganisms enclosed in an extracellular polymeric material (EPS), hold immense importance the fields environmental research, industry, and medicine. They play a significant role ecosystem dynamics stability, but they also pose issues such as biofouling, corrosion, pollution. Biofilms medical environments are linked to persistent infections elevated healthcare expenses. The EPS matrix plays crucial maintaining structural integrity antibiotic resistance these structures. research primarily investigates facilitating horizontal gene transfer among biofilm communities, with particular emphasis on its impact this process. process is recognized pivotal mechanism emergence resistance, underscoring function biofilms. analysis highlights financial constraints caused by biofilms several industries. Biofilm-associated sector result escalated treatment expenses extended hospitalization periods. In industrial context, have increasing maintenance product contamination, emphasizing need for efficient management solutions. This review presents most recent progress utilization sophisticated imaging tools molecular methodologies. addition conventional techniques, explores tools, DNA RNA sequencing, conjunction proteomics. These approaches essential assessing genetic metabolic mechanisms that regulate development resistance. underscores significance employing interdisciplinary methodology study By incorporating range approaches, analysis, comprehensive understanding may be achieved. approach opens up possibilities developing novel solutions address negative impacts health, environment.

Language: Английский

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Understanding biofouling and contaminant accretion on submerged marine structures

npj Materials Degradation, Journal Year: 2023, Volume and Issue: 7(1)

Published: June 21, 2023

Abstract Fouling of maritime infrastructure is pervasive due to abundant biological and chemical activity within the oceanic environment. Marine biofilms their successional growths are prevalent issues in biofouling, but current industrial research-based analyses often do not provide a holistic view fouling biodiversity. Cathodic protection longstanding system safeguarding from corrosive marine environment, limited studies on interactions between growth cathodic have been conducted context fouling. This review identifies knowledge gaps understanding highlights approaches better direct development effective anti-fouling measures.

Language: Английский

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Artificial Intelligence-Driven Analysis of Antimicrobial-Resistant and Biofilm-Forming Pathogens on Biotic and Abiotic Surfaces

Antibiotics, Journal Year: 2024, Volume and Issue: 13(8), P. 788 - 788

Published: Aug. 22, 2024

The growing threat of antimicrobial-resistant (AMR) pathogens to human health worldwide emphasizes the need for more effective infection control strategies. Bacterial and fungal biofilms pose a major challenge in treating AMR pathogen infections. Biofilms are formed by pathogenic microbes encased extracellular polymeric substances confer protection from antimicrobials host immune system. also promote growth antibiotic-resistant mutants latent persister cells thus complicate therapeutic approaches. ubiquitous cause serious risks due their ability colonize various surfaces, including tissues, medical devices, food-processing equipment. Detection characterization crucial prompt intervention control. To this end, traditional approaches often effective, yet they fail identify microbial species inside biofilms. Recent advances artificial intelligence (AI) have provided new avenues improve biofilm identification. Machine-learning algorithms image-processing techniques shown promise accurate efficient detection biofilm-forming microorganisms on biotic abiotic surfaces. These advancements potential transform research clinical practice allowing faster diagnosis tailored therapy. This comprehensive review focuses application AI identification industries, healthcare, food safety, agriculture. discusses existing approaches, challenges, applications research, with particular focus role improving diagnostic capacities guiding preventative actions. synthesis current knowledge future directions, as described review, will guide development efforts combating biofilm-associated

Language: Английский

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Synergistic interactions in multispecies biofilm combinations of bacterial isolates recovered from diverse food processing industries

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 13, 2023

Most biofilms within the food industry are formed by multiple bacterial species which co-exist on surfaces as a result of interspecies interactions. These ecological interactions often make these communities tolerant against antimicrobials. Our previous work led to identification large number (327) highly diverse contact dairy, meat, and egg industries after routine cleaning disinfection (C&D) regimes. In current study, biofilm-forming ability 92 strains belonging 26 genera 42 was assessed synergistic in biofilm formation were investigated coculturing all possible four-species combinations. Out total 455 combinations, greater mass production, compared sum masses individual monoculture, observed 34 Around half combinations showed synergy > 1.5-fold most belonged dairy strains. The highest (3.13-fold) shown combination comprising Stenotrophomonas rhizophila, Bacillus licheniformis, Microbacterium lacticum, Calidifontibacter indicus. mixed turned out be strain-specific rather than species-dependent. All showing remarkable appeared have certain common shows there keystone industry-specific stimulate or antagonism this may implication for control concerned industries.

Language: Английский

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Electrochemical Impedance Spectroscopy-Based Sensing of Biofilms: A Comprehensive Review

Biosensors, Journal Year: 2023, Volume and Issue: 13(8), P. 777 - 777

Published: July 31, 2023

Biofilms are complex communities of microorganisms that can form on various surfaces, including medical devices, industrial equipment, and natural environments. The presence biofilms lead to a range problems, infections, reduced efficiency failure biofouling or spoilage, environmental damage. As result, there is growing need for tools measure monitor levels in biomedical, pharmaceutical, food processing settings. In recent years, electrochemical impedance sensing has emerged as promising approach real-time, non-destructive, rapid monitoring biofilms. This article sheds light measuring biofilms, its high sensitivity, non-destructive nature, versatility, low cost, real-time capabilities. We also discussed some applications studying medical, environmental, presents future perspectives research would the creation reliable, quick, easy-to-use biosensors mounted unmanned aerial vehicles (UAVs), ground (UGVs), utilizing artificial intelligence-based terminologies detect

Language: Английский

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Tools of the Trade: Image Analysis Programs for Confocal Laser-Scanning Microscopy Studies of Biofilms and Considerations for Their Use by Experimental Researchers

ACS Omega, Journal Year: 2023, Volume and Issue: 8(23), P. 20163 - 20177

Published: May 26, 2023

Confocal laser-scanning microscopy (CLSM) is the bedrock of microscopic visualization biofilms. Previous applications CLSM in biofilm studies have largely focused on observations bacterial or fungal elements biofilms, often seen as aggregates mats cells. However, field research moving beyond qualitative alone, toward quantitative analysis structural and functional features across clinical, environmental, laboratory conditions. In recent times, several image programs been developed to extract quantify properties from confocal micrographs. These tools not only vary their scope relevance specific under study but also with respect user interface, compatibility operating systems, raw requirements. Understanding these considerations important when selecting for analysis, including at initial experimental stages acquisition. this review, we provide an overview micrographs a focus tool selection acquisition parameters that are relevant researchers ensure reliability downstream processing.

Language: Английский

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Interspecies interactions in dairy biofilms drive community structure and response against cleaning and disinfection

Biofilm, Journal Year: 2024, Volume and Issue: 7, P. 100195 - 100195

Published: April 8, 2024

Interspecies interactions within a biofilm community influence population dynamics and structure, which in turn may affect the bacterial stress response to antimicrobials. This study was conducted assess impact of between

Language: Английский

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Global challenges and microbial biofilms: Identification of priority questions in biofilm research, innovation and policy

Biofilm, Journal Year: 2024, Volume and Issue: 8, P. 100210 - 100210

Published: July 4, 2024

Priority question exercises are increasingly used to frame and set future research, innovation development agendas. They can provide an important bridge between the discoveries, data outputs generated by researchers, information required policy makers funders. Microbial biofilms present huge scientific, societal economic opportunities challenges. In order identify key priorities that will help advance field, here we review questions from a pool submitted international biofilm research community practitioners working across industry, environment medicine. To avoid bias computational approaches group manage voting selection process. The outcome of exercise is 78 unique questions, categorized in six themes: (i) Biofilm control, disruption, prevention, management, treatment (13 questions); (ii) Resistance, persistence, tolerance, role aggregation, immune interaction, relevance infection (10 (iii) Model systems, standards, regulatory, education, interdisciplinary (15 (iv) Polymicrobial, interactions, ecology, microbiome, phage (v) Clinical focus, chronic infection, detection, diagnostics (vi) Matrix, lipids, capsule, metabolism, development, physiology, evolution environment, engineering (14 questions). presented intended highlight opportunities, stimulate discussion focus for funders makers, informing strategy microbial communities.

Language: Английский

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Intestinal biofilms: pathophysiological relevance, host defense, and therapeutic opportunities

Clinical Microbiology Reviews, Journal Year: 2024, Volume and Issue: 37(3)

Published: July 12, 2024

SUMMARY The human intestinal tract harbors a profound variety of microorganisms that live in symbiosis with the host and each other. It is complex highly dynamic environment whose homeostasis directly relates to health. Dysbiosis gut microbiota polymicrobial biofilms have been associated gastrointestinal diseases, including irritable bowel syndrome, inflammatory colorectal cancers. This review covers molecular composition organization biofilms, mechanistic aspects biofilm signaling networks for bacterial communication behavior, synergistic effects biofilms. further describes clinical relevance diseases role antimicrobial resistance, defense system therapeutic strategies counteracting Taken together, this summarizes latest knowledge research on their disorders provides directions toward development biofilm-specific treatments.

Language: Английский

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