Disentangling arbuscular mycorrhizal fungi and bacteria at the soil-root interface

Mycorrhiza, Journal Year: 2023, Volume and Issue: 33(3), P. 119 - 137

Published: March 24, 2023

Language: Английский

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Fungal-fungal cocultivation leads to widespread secondary metabolite alteration requiring the partial loss-of-function VeA1 protein

Science Advances, Journal Year: 2022, Volume and Issue: 8(17)

Published: April 27, 2022

Microbial communication has attracted notable attention as an indicator of microbial interactions that lead to marked alterations secondary metabolites (SMs) in varied environments. However, the mechanisms responsible for SM regulation are not fully understood, especially fungal-fungal interactions. Here, cocultivation endophytic fungus Epicoccum dendrobii with model Aspergillus nidulans and several other filamentous fungi triggered widespread alteration SMs. Multiple silent biosynthetic gene clusters A. were activated by transcriptome metabolome analysis. Unprecedentedly, deletion replacement proved a partial loss-of-function VeA1 protein, but VeA, was associated changes both fumigatus during cocultivation. required transcription factor SclB velvet complex members LaeA VelB producing aspernidines representative formation SMs . This study provides new insights into mechanism trigger metabolic

Language: Английский

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Soil microbiome bacteria protect plants against filamentous fungal infections via intercellular contacts

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(3)

Published: Jan. 15, 2025

Bacterial–fungal interaction (BFI) has significant implications for the health of host plants. While diffusible antibiotic metabolite-mediated competition in BFI been extensively characterized, impact intercellular contact remains largely elusive. Here, we demonstrate that is a prevalent mode between beneficial soil bacteria and pathogenic filamentous fungi. By generating antibiotics-deficient mutants two common bacteria, Lysobacter enzymogenes Pseudomonas fluorescens , show antibiotics-independent effectively inhibits Furthermore, transcriptional genetic evidence revealed this relies on mediated by type VI secretion system (T6SS), which may facilitate translocation bacterial toxic effectors into fungal cells. Finally, using “conidia enrichment” platform, found T6SS-mediated inhibition resulting from naturally occurs within microbiome, particularly represented fulva . Overall, these results microbiome can protect plants infection through contacts, thus revealing occurring ecologically important agricultural contexts.

Language: Английский

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Chemical signals driving bacterial–fungal interactions

Environmental Microbiology, Journal Year: 2021, Volume and Issue: 23(3), P. 1334 - 1347

Published: Jan. 29, 2021

Microorganisms reside in diverse environmental communities where interactions become indispensable due to close physical associations. These are driven by chemical communication among different microbial kingdoms, particularly between fungi and bacteria. Knowledge about these signals provides useful information the nature of allows predictions community development environments. Here, we provide an update on role small signalling molecules fungal-bacterial with focus agricultural medicinal This review highlights range - response biochemicals produced both kingdoms view harnessing their properties towards drug discovery applications.

Language: Английский

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The phosphate language of fungi

Trends in Microbiology, Journal Year: 2021, Volume and Issue: 30(4), P. 338 - 349

Published: Aug. 31, 2021

Language: Английский

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Bacterial‐fungal interactions and their impact on microbial pathogenesis

Molecular Ecology, Journal Year: 2022, Volume and Issue: 32(10), P. 2565 - 2581

Published: March 1, 2022

Abstract Microbial communities of the human microbiota exhibit diverse effects on health and disease. homeostasis is important for normal physiological functions changes to are associated with many diseases including diabetes, cancer, colitis. In addition, there microorganisms that either commensal or acquired from environmental reservoirs can cause pathologies. Importantly, balance between disease intricately connected how members interact affect one another's growth pathogenicity. However, mechanisms govern these interactions only beginning be understood. this review, we outline bacterial‐fungal in body, examining by which bacteria fungal virulence, as well fungi regulate bacterial pathogenesis. We summarize advances understanding chemical, physical, protein‐based interactions, their role exacerbating impeding focus three species responsible majority systemic infections humans: Candida albicans , Cryptococcus neoformans Aspergillus fumigatus . conclude summarizing recent studies have mined microbes novel antimicrobials antivirulence factors, highlighting potential a rich resource small molecule discovery.

Language: Английский

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Disease Inhibiting Effect of Strain Bacillus subtilis EG21 and Its Metabolites Against Potato Pathogens Phytophthora infestans and Rhizoctonia solani

Phytopathology, Journal Year: 2022, Volume and Issue: 112(10), P. 2099 - 2109

Published: May 10, 2022

Potato production worldwide is plagued by several disease-causing pathogens that result in crop and economic losses estimated to billions of dollars each year. To this day, synthetic chemical applications remain the most widespread control strategy despite their negative effects on human environmental health. Therefore, obtainment superior biocontrol agents or naturally produced metabolites replace fungicides be integrated into practical pest management strategies has become one main targets modern agriculture. Our focus present study was elucidate antagonistic potential a new strain identified as Bacillus subtilis EG21 against potato Phytophthora infestans Rhizoctonia solani using vitro screening assays. Microscopic examination interaction between R. showed extended damage fungal mycelium, while displayed strong anti-oomycete zoosporecidal effect P. infestans. Mass spectrometry (MS) analysis revealed antifungal cyclic lipopeptides surfactins (C12 C19). Further characterization confirmed its ability produce siderophores extracellular lytic enzymes cellulase, pectinase chitinase. The activity cell-free culture filtrate (CF) found stable at high-temperature/pressure treatment extreme pH values not affected proteinase K treatment. Disease-inhibiting CF infection leaves tubers, respectively. Biotechnological microbial bioproducts for protection hold great promise develop effective, environment-friendly sustainable strategies. [Formula: see text] Copyright © 2022 Author(s). This an open access article distributed under CC BY-NC-ND 4.0 International license.

Language: Английский

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The pathobiome concept applied to postharvest pathology and its implication on biocontrol strategies

Postharvest Biology and Technology, Journal Year: 2022, Volume and Issue: 189, P. 111911 - 111911

Published: April 1, 2022

Language: Английский

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Bacterial-fungal interactions under agricultural settings: from physical to chemical interactions

Stress Biology, Journal Year: 2022, Volume and Issue: 2(1)

Published: June 1, 2022

Abstract Bacteria and fungi are dominant members of environmental microbiomes. Various bacterial-fungal interactions (BFIs) their mutual regulation important factors for ecosystem functioning health. Such can be highly dynamic, often require spatiotemporally resolved assessments to understand the interplay which ranges from antagonism mutualism. Many these still poorly understood, especially in terms underlying chemical molecular interplay, is crucial inter-kingdom communication interference. BFIs relevant under agricultural settings; they determinative crop Advancing our knowledge related mechanisms underpinning between bacteria will provide an extended basis biological control pests pathogens agriculture. Moreover, it facilitate a better understanding complex microbial community networks that commonly occur nature. This allow us determine assembly different conditions pave way constructing synthetic communities various biotechnological applications. Here, we summarize current advances field with emphasis on

Language: Английский

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The effects of NDM-5 on Escherichia coli and the screening of interacting proteins

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 30, 2024

Carbapenem-resistant Escherichia coli ( E. ) strains are widely distributed and spreading rapidly, creating significant challenges for clinical therapeutics. NDM-5, a novel mutant of New Delhi Metallo-β-Lactamase-1 (NDM-1), exhibits high hydrolase activity toward carbapenems. Since the genetic backgrounds clinically isolated carbapenem-resistant heterogeneous, it is difficult to accurately evaluate impact bla NDM–5 on antibiotic resistance. Herein, BL21 was transformed with plasmid harboring , resultant strain named (pET-28a- ). Consistent findings previous studies, introduction exogenous resulted in markedly greater resistance multiple β-lactam antibiotics. Compared (pET-28a), exhibited reduced motility but increase biofilm formation capacity. Furthermore, transcriptome sequencing conducted compare transcriptional differences between (pET-28a) A total 461 differentially expressed genes were identified, including those related resistance, such as associated active efflux system yddA mcbR emrY ), pili csgC csgF fimD csgD csgB ecpR antioxidant processes nuoG Finally, pGS21a into Rosetta2, after which expression NDM-5 protein induced using isopropyl-β-D-thiogalactoside (IPTG). Using glutathione-S-transferase (GST) pull-down assays, proteins from scanned screen out 82 that potentially interacted NDM-5. Our provide new insight identified identify potential targets design inhibitors bacteria.

Language: Английский

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