The Role of Air Relative Humidity on the Wettability of <i>Pseudomonas Fluorescens</i> AR11 Biofilms DOI
Daniele Marra,

Emilia Orillo,

Giuseppe Toscano

et al.

Published: Jan. 1, 2023

Biofilms are complex porous materials formed by microorganisms, polysaccharides, proteins, eDNA, inorganic matter, and water. They ubiquitous in various environmental niches known to grow at solid-liquid, solid-air air-liquid interfaces, often causing problems several industrial sanitary fields. Their removal is a challenge many applications numerous studies have been conducted identify promising chemical species as cleaning agents. While these substances target specific components of biofilm structure, the role water content biofilm, how it can influence wettability detergent absorption quite neglected literature. Estimating challenging task due its heterogeneity morphology composition. In this study, we controlled Pseudomonas fluorescens AR 11 biofilms grown on submerged glass slides regulating relative humidity after drying. Interfacial properties were investigated measuring wetting soybean oil. The structure was evaluated using Confocal Laser Scanning Microscopy Electron Microscopy. results showed that has significant measurable effect wettability, leading hypothesis preliminary control play crucial process.

Language: Английский

Low-shear modeled microgravity accelerated the degradation of passive films of aluminium alloy by Bacillus cereus through increased extracellular polymeric substances DOI
Yali Feng,

Junhang Chen,

Qianqian Liu

et al.

Applied Surface Science, Journal Year: 2024, Volume and Issue: 655, P. 159618 - 159618

Published: Feb. 7, 2024

Language: Английский

Citations

8
Direct comparison of spatial transcriptional heterogeneity across diverse Bacillus subtilis biofilm communities DOI Creative Commons
Yasmine Dergham, Dominique Le Coq, Pierre Nicolas

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 20, 2023

Bacillus subtilis can form various types of spatially organised communities on surfaces, such as colonies, pellicles and submerged biofilms. These share similarities differences, phenotypic heterogeneity has been reported for each type community. Here, we studied spatial transcriptional across the three surface-associated communities. Using RNA-seq analysis different regions or populations community type, identified genes that are specifically expressed within selected population. We constructed fluorescent fusions 17 these genes, observed their expression in biofilms using time-lapse confocal laser scanning microscopy (CLSM). found mosaic patterns some genes; particular, segregated cells displaying opposite regulation carbon metabolism (gapA gapB), indicative distinct glycolytic gluconeogenic regimes coexisting same biofilm region. Overall, our study provides a direct comparison heterogeneity, at scales, main models B.

Language: Английский

Citations

15
Biofilm contamination in confined space stations: reduction, coexistence or an opportunity? DOI Creative Commons
Daniele Marra, Rosalia Ferraro, Sergio Caserta

et al.

Frontiers in Materials, Journal Year: 2024, Volume and Issue: 11

Published: March 28, 2024

The prolonged human permanence in confined environments space, such as the case of International Space Station, has inadvertently fostered conditions leading to uncontrolled microbial proliferation on surfaces, known biofilm. Biofilm presence represents a challenge critical spacecraft systems, that can lead contamination issues and systems loss function due biofouling phenomena. This scenario is further complicated by microgravity controversial role biofilm growth formation. Biocontamination be limiting factor long-term mission outer Earth orbit an economic health issue ISS. study addresses pressing need for effective antimicrobial strategies against resilient biofilms where usage biocidal chemical compounds strictly controlled toxicity dangers. Traditional methods complemented advanced coatings techniques. A promising approach based oxygen plasma coating platform. technology potentially extended wide range antibiofilm agents (e.g., peptides, bacteriophages, nanoparticles, quorum sensing disrupting agents, etc.) substrates metal, plastic, ceramic) showing exceptional flexibility. An alternative vision inspired dual nature biofilms, addressed “good” or “bad” depending specific application. Indeed, have great potential closed small space habitat ISS) their “large habitat” planet itself. replication complex biological equilibrium open challenge.

Language: Английский

Citations

4
Island archaeology provides ecological and behavioral analogs for off-planet exploration and colonization DOI
Thomas P. Leppard, Scott M. Fitzpatrick

Futures, Journal Year: 2025, Volume and Issue: unknown, P. 103544 - 103544

Published: Jan. 1, 2025

Language: Английский

Citations

0
Spaceflight associated dry eye syndrome (SADES): Outflow biophysics and infection risk DOI
Ryung Lee, Joshua Ong, Ethan Waisberg

et al.

Journal of Space Safety Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Nanoparticle Coatings on Glass Surfaces to Prevent Pseudomonas fluorescens AR 11 Biofilm Formation DOI Creative Commons
Daniele Marra, Irene Perna, Giulio Pota

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(3), P. 621 - 621

Published: Feb. 28, 2023

Microbial colonization of surfaces is a sanitary and industrial issue for many applications, leading to product contamination human infections. When microorganisms closely interact with surface, they start produce an exo-polysaccaridic matrix adhere protect themselves from adverse environmental conditions. This type structure called biofilm. The aim our work investigate novel technologies able prevent biofilm formation by surface coatings. We coated glass melanin-ZnO2, melanin-TiO2, TiO2 hybrid nanoparticles. functionalization was performed using cold plasma activate glass-substrate-coated surfaces, that were characterized performing water soybean oil wetting tests. A quantitative characterization the antibiofilm properties done Pseudomonas fluorescens AR 11 as model organism. Biofilm morphologies observed confocal laser scanning microscopy image analysis techniques used obtain morphological parameters. results highlight efficacy proposed coating formation. Melanin-TiO2 proved be most efficient among particles investigated. Our can valuable support future implementation technique here in extended range applications may include further testing on other strains materials.

Language: Английский

Citations

9
Bacterial biofilm-mediated environmental remediation: Navigating strategies to attain Sustainable Development Goals DOI
Surajit Das,

Trisnehi Pradhan,

Sourav Kumar Panda

et al.

Journal of Environmental Management, Journal Year: 2024, Volume and Issue: 370, P. 122745 - 122745

Published: Oct. 9, 2024

Language: Английский

Citations

3
Bacillus subtilis NDmed, a model strain for biofilm genetic studies DOI Creative Commons
Yasmine Dergham, Dominique Le Coq, Arnaud Bridier

et al.

Biofilm, Journal Year: 2023, Volume and Issue: 6, P. 100152 - 100152

Published: Aug. 29, 2023

The Bacillus subtilis strain NDmed was isolated from an endoscope washer-disinfector in a medical environment. can form complex macrocolonies with highly wrinkled architectural structures on solid medium. In static liquid culture, it produces thick pellicles at the interface air as well remarkable protruding ''beanstalk-like'' submerged biofilm surface. Since these mucoid are hyper-resistant to biocides, has ability protect pathogens embedded mixed-species biofilms by sheltering them action of agents. Additionally, this non-domesticated and forming propensity being genetically manipulated. Due all properties, becomes valuable model for study B. biofilms. This review focuses several studies performed that have highlighted sophisticated genetic dynamics play during formation. Further project using modern molecular tools advanced technologies strain, will allow deepen our knowledge emerging properties multicellular bacterial life.

Language: Английский

Citations

6
Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin DOI Creative Commons
Stefano Serpelloni,

Michael Ellis Williams,

Sergio Caserta

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(10), P. 11701 - 11717

Published: Feb. 28, 2024

As the population ages, number of vascular surgery procedures performed increases. Older adults often have multiple comorbidities, such as diabetes and hypertension, that increase risk complications from including graft infection (VGI). VGI is a serious complication with significant morbidity, mortality, healthcare costs. Here, we aimed to develop nanofibrous chitosan-based coating for grafts loaded different concentrations vancomycin antibiotic (VAN). Blending chitosan poly(vinyl alcohol) or poly(ethylene oxide) copolymers improved solubility ease spinning. Thermal gravimetric analysis Fourier transform infrared spectroscopy confirmed presence VAN in membranes. Kinetics release mats were evaluated using high-performance liquid chromatography, showing burst followed by sustained over 24 h. To achieve longer release, poly(lactic-co-glycolic acid) was applied, resulting extended up 7 days. Biocompatibility assessment human umbilical vein endothelial cells demonstrated successful attachment viability nanofiber patches. Our study provides insights into development drug delivery system at preventing during implantation, highlighting potential electrospinning promising technique field surgery.

Language: Английский

Citations

2
Benefits of a rotating – Partial gravity – Spacecraft DOI Creative Commons
Jack J. W. A. van Loon,

Cesare Lobascio,

Giorgio Boscheri

et al.

Acta Astronautica, Journal Year: 2024, Volume and Issue: 220, P. 403 - 415

Published: May 6, 2024

A long-duration microgravity environment has numerous detrimental effects on the human physiology. The most obvious solution for this problem related to long duration space exploration missions is remedy lack of gravity. This could be done using short arm centrifuges but they do not seem sufficiently effective, perhaps because exposure countermeasure and / or huge body gravity gradient. New views have investigated, such as see if a (very) long-arm rotating system generating continuous 1g partial field might resolve issue. Besides expected benefits regarding astronauts' pathologies, additionally spacecraft itself, its on-board (sub-)systems procedures benefit from configuration. In paper we address very briefly medical issues, work mainly focused advantages engineering, operations, life support, safety budget having constantly first in Low Earth Orbit later Mars. large feasible affordable build, operate maintain. It governmental commercial use also light increase tourism. will save crew time billions dollars now being spent counteract microgravity.

Language: Английский

Citations

2