Microfluidic technologies for nanoparticle formation DOI
Fei Tian, Lili Cai, Chao Liu

et al.

Lab on a Chip, Journal Year: 2021, Volume and Issue: 22(3), P. 512 - 529

Published: Nov. 1, 2021

Functional nanoparticles (NPs) hold immense promise in diverse fields due to their unique biological, chemical, and physical properties associated with size or morphology. Microfluidic technologies featuring precise fluid manipulation have become versatile toolkits for manufacturing NPs a highly controlled manner low batch-to-batch variability. In this review, we present the fundamentals of microfluidic fabrication strategies, including mixing-, droplet-, multiple field-based methods. We highlight formation functional using these reactors, an emphasis on lipid NPs, polymer lipid-polymer hybrid supramolecular metal metal-oxide metal-organic framework covalent organic quantum dots, perovskite nanocrystals, biomimetic etc. discuss future directions accelerated development such as device parallelization large-scale NP production, efficient optimization formulations, AI-guided design multi-step reactors.

Language: Английский

mRNA vaccines for infectious diseases: principles, delivery and clinical translation DOI Open Access
Namit Chaudhary, Drew Weissman, Kathryn A. Whitehead

et al.

Nature Reviews Drug Discovery, Journal Year: 2021, Volume and Issue: 20(11), P. 817 - 838

Published: Aug. 25, 2021

Language: Английский

Citations

1005
Lipid Nanoparticles for Drug Delivery DOI Creative Commons
Letao Xu, Xing Wang, Yun Liu

et al.

Advanced NanoBiomed Research, Journal Year: 2021, Volume and Issue: 2(2)

Published: Nov. 25, 2021

Lipid nanoparticles have attracted significant interests in the last two decades, and achieved tremendous clinical success since first approval of Doxil 1995. At same time, lipid also demonstrated enormous potential delivering nucleic acid drugs as evidenced by RNA therapies mRNA COVID‐19 vaccines. In this review, an overview on different classes nanoparticles, including liposomes, solid nanostructured carriers, is provided, followed introduction their preparation methods. Then characterizations are briefly reviewed applications encapsulating hydrophobic drugs, hydrophilic RNAs highlighted. Finally, various for overcoming delivery challenges, crossing blood–brain barrier, targeted delivery, routes administration, summarized. drug systems offer many attractive benefits such great biocompatibility, ease preparation, feasibility scale‐up, nontoxicity, while current challenges warrant future studies about structure–function correlations, large‐scale production, to realize full wider pharmaceutical future.

Language: Английский

Citations

313
Preparation of selective organ-targeting (SORT) lipid nanoparticles (LNPs) using multiple technical methods for tissue-specific mRNA delivery DOI Open Access
Xu Wang, Shuai Liu, Yehui Sun

et al.

Nature Protocols, Journal Year: 2022, Volume and Issue: 18(1), P. 265 - 291

Published: Oct. 31, 2022

Language: Английский

Citations

254
A Comprehensive Review of mRNA Vaccines DOI Open Access
Vrinda Gote, Pradeep Kumar Bolla, Nagavendra Kommineni

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2700 - 2700

Published: Jan. 31, 2023

mRNA vaccines have been demonstrated as a powerful alternative to traditional conventional because of their high potency, safety and efficacy, capacity for rapid clinical development, potential rapid, low-cost manufacturing. These progressed from being mere curiosity emerging COVID-19 pandemic vaccine front-runners. The advancements in the field nanotechnology developing delivery vehicles are highly significant. In this review we summarized each every aspect vaccine. article describes structure, its pharmacological function immunity induction, lipid nanoparticles (LNPs), upstream, downstream, formulation process Additionally, trials also described. A deep dive into future perspectives vaccines, such freeze-drying, systems, LNPs targeting antigen-presenting cells dendritic cells, summarized.

Language: Английский

Citations

209
Scalable mRNA and siRNA Lipid Nanoparticle Production Using a Parallelized Microfluidic Device DOI
Sarah J. Shepherd, Claude C. Warzecha, Sagar Yadavali

et al.

Nano Letters, Journal Year: 2021, Volume and Issue: 21(13), P. 5671 - 5680

Published: June 30, 2021

A major challenge to advance lipid nanoparticles (LNPs) for RNA therapeutics is the development of formulations that can be produced reliably across various scales drug development. Microfluidics generate LNPs with precisely defined properties, but have been limited by challenges in scaling throughput. To address this challenge, we present a scalable, parallelized microfluidic device (PMD) incorporates an array 128 mixing channels operate simultaneously. The PMD achieves >100× production rate compared single channels, without sacrificing desirable LNP physical properties and potency typical microfluidic-generated LNPs. In mice, show superior delivery encapsulating either Factor VII siRNA or luciferase-encoding mRNA generated using conventional mixing, 4-fold increase hepatic gene silencing 5-fold luciferase expression, respectively. These results suggest scalable reproducible needed emerging clinical applications, including vaccines.

Language: Английский

Citations

202
Lipid-Based Nanoparticles for Drug/Gene Delivery: An Overview of the Production Techniques and Difficulties Encountered in Their Industrial Development DOI Creative Commons
Meenu Mehta, Thuỳ Anh Bùi,

Xinpu Yang

et al.

ACS Materials Au, Journal Year: 2023, Volume and Issue: 3(6), P. 600 - 619

Published: Aug. 21, 2023

Over the past decade, therapeutic potential of nanomaterials as novel drug delivery systems complementing conventional pharmacology has been widely acknowledged. Among these nanomaterials, lipid-based nanoparticles (LNPs) have shown remarkable pharmacological performance and promising outcomes, thus gaining substantial interest in preclinical clinical research. In this review, we introduce main types LNPs used formulations such liposomes, nanoemulsions, solid lipid nanoparticles, nanostructured carriers, polymer hybrid focusing on their physicochemical properties potential. We discuss computational studies modeling techniques to enhance understanding how interact with cargo predict effectiveness interactions applications. also analyze benefits drawbacks various LNP production nanoprecipitation, emulsification, evaporation, thin film hydration, microfluidic-based methods, an impingement jet mixer. Additionally, major challenges associated industrial development, including stability sterilization, storage, regulatory compliance, reproducibility, quality control. Overcoming facilitating compliance represent key steps toward LNP's successful commercialization translation into settings.

Language: Английский

Citations

179
Microfluidic Nanoparticles for Drug Delivery DOI Creative Commons
Yun Liu, Guangze Yang, Yue Hui

et al.

Small, Journal Year: 2022, Volume and Issue: 18(36)

Published: April 9, 2022

Abstract Nanoparticles (NPs) have attracted tremendous interest in drug delivery the past decades. Microfluidics offers a promising strategy for making NPs due to its capability precisely controlling NP properties. The recent success of mRNA vaccines using microfluidics represents big milestone microfluidic pharmaceutical applications, and rapid scaling up demonstrates feasibility industrial‐scale manufacturing. This article provides critical review progress delivery. First, synthesis organic focusing on typical methods their applications popular clinically relevant NPs, such as liposomes, lipid polymer well mechanisms are summarized. Then, several representative inorganic (e.g., silica, metal, metal oxide, quantum dots), hybrid is discussed. Lastly, various presented.

Language: Английский

Citations

174
Nanoparticles: From synthesis to applications and beyond DOI
Atiđa Selmani, Davor Kovačević, Klemen Bohinc

et al.

Advances in Colloid and Interface Science, Journal Year: 2022, Volume and Issue: 303, P. 102640 - 102640

Published: March 10, 2022

Language: Английский

Citations

129
Deploying Gold Nanomaterials in Combating Multi-Drug-Resistant Bacteria DOI
Xiaohui Zhao, Hao Tang, Xingyu Jiang

et al.

ACS Nano, Journal Year: 2022, Volume and Issue: 16(7), P. 10066 - 10087

Published: July 1, 2022

Antibiotic resistance has become a serious threat to human health due the overuse of antibiotics. Different antibiotics are being developed treat resistant bacteria, but development cycle is hard keep up with high incidence antibiotic resistance. Recent advances in antimicrobial nanomaterials have made nanotechnology powerful solution this dilemma. Among these nanomaterials, gold excellent antibacterial efficacy and biosafety, making them alternatives This review presents strategies that use combat drug-resistant bacteria. We focus on influence physicochemical factors such as surface chemistry, size, shape their properties describe applications medical devices. Finally, existing challenges future directions discussed.

Language: Английский

Citations

106
Ligand-tethered lipid nanoparticles for targeted RNA delivery to treat liver fibrosis DOI Creative Commons
Xuexiang Han, Ningqiang Gong, Lulu Xue

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 17, 2023

Abstract Lipid nanoparticle-mediated RNA delivery holds great potential to treat various liver diseases. However, targeted of therapeutics activated liver-resident fibroblasts for fibrosis treatment remains challenging. Here, we develop a combinatorial library anisamide ligand-tethered lipidoids (AA-lipidoids) using one-pot, two-step modular synthetic method and adopt two-round screening strategy identify AA-lipidoids with both high potency selectivity deliver payloads fibroblasts. The lead AA-lipidoid AA-T3A-C12 mediates greater transfection than its analog without the FDA-approved MC3 ionizable lipid. In preclinical model fibrosis, enables ~65% silencing heat shock protein 47, therapeutic target primarily expressed by fibroblasts, which is 2-fold more potent MC3, leading significantly reduced collagen deposition fibrosis. These results demonstrate Furthermore, these methods strategies open new avenue discover targeting properties, can potentially enable range cell tissue types that are challenging access traditional lipid nanoparticle formulations.

Language: Английский

Citations

96