Organoids: The current status and biomedical applications

MedComm, Journal Year: 2023, Volume and Issue: 4(3)

Published: May 17, 2023

Organoids are three-dimensional (3D) miniaturized versions of organs or tissues that derived from cells with stem potential and can self-organize differentiate into 3D cell masses, recapitulating the morphology functions their in vivo counterparts. Organoid culture is an emerging technology, organoids various tissues, such as brain, lung, heart, liver, kidney, have been generated. Compared traditional bidimensional culture, organoid systems unique advantage conserving parental gene expression mutation characteristics, well long-term maintenance function biological characteristics vitro. All these features open up new opportunities for drug discovery, large-scale screening, precision medicine. Another major application disease modeling, especially hereditary diseases difficult to model vitro modeled by combining genome editing technologies. Herein, we introduce development current advances technology field. We focus on applications basic biology clinical research, also highlight limitations future perspectives. hope this review provide a valuable reference developments organoids.

Language: Английский

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Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Language: Английский

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Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering

Journal of Functional Biomaterials, Journal Year: 2022, Volume and Issue: 13(4), P. 240 - 240

Published: Nov. 14, 2022

The multidisciplinary fields of tissue engineering and regenerative medicine have the potential to revolutionize practise through abilities repair, regenerate, or replace tissues organs with functional engineered constructs. To this end, combines scaffolding materials cells biologically active molecules into constructs appropriate structures properties for tissue/organ regeneration, where biomolecules are keys mimic native extracellular matrix (ECM). For this, one emerging way is decellularize ECM suitable for, directly in combination other materials, creating Over past decade, decellularized (or dECM) has greatly facilitated advance medicine, while being challenged many ways. This article reviews recent development dECM a focus on preparation along its influence cell culture, modification use as material, novel techniques trends processing We highlight success vitro, vivo, clinical applications further identify key issues challenges involved, discussion future research directions.

Language: Английский

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Recent advances in defined hydrogels in organoid research

Bioactive Materials, Journal Year: 2023, Volume and Issue: 28, P. 386 - 401

Published: June 16, 2023

Organoids are in vitro model systems that mimic the complexity of organs with multicellular structures and functions, which provide great potential for biomedical tissue engineering. However, their current formation heavily relies on using complex animal-derived extracellular matrices (ECM), such as Matrigel. These often poorly defined chemical components exhibit limited tunability reproducibility. Recently, biochemical biophysical properties hydrogels can be precisely tuned, offering broader opportunities to support development maturation organoids. In this review, fundamental ECM vivo critical strategies design organoid culture summarized. Two typically derived from natural synthetic polymers applicability improve organoids presented. The representative applications incorporating into highlighted. Finally, some challenges future perspectives also discussed developing advanced technologies toward supporting research.

Language: Английский

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3D Biomimetic Models to Reconstitute Tumor Microenvironment In Vitro: Spheroids, Organoids, and Tumor‐on‐a‐Chip

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(18)

Published: March 14, 2023

Abstract Decades of efforts in engineering vitro cancer models have advanced drug discovery and the insight into biology. However, establishment preclinical that enable fully recapitulating tumor microenvironment remains challenging owing to its intrinsic complexity. Recent progress techniques has allowed development a new generation can recreate complex vivo microenvironments accurately predict responses, including spheroids, organoids, tumor‐on‐a‐chip. These biomimetic 3D are particular interest as they pave way for better understanding biology accelerating anticancer therapeutics with reducing animal use. Here, recent advances developing these platforms modeling screening, focusing on incorporating hydrogels reviewed reconstitute physiologically relevant microenvironments. The combination spheroids/organoids microfluidic technologies is also highlighted mimic tumors discuss challenges future directions clinical translation such screening personalized medicine.

Language: Английский

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Clinical applications of human organoids

Nature Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

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Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices

Bioengineering, Journal Year: 2025, Volume and Issue: 12(2), P. 136 - 136

Published: Jan. 30, 2025

Whole-organ decellularization generates scaffolds containing native extracellular matrix (ECM) components with preserved tissue microarchitecture, providing a promising advancement in engineering and regenerative medicine. Decellularization retains the ECM integrity which is important for supporting cell attachment, growth, differentiation, biological function. Although there are consensus guidelines to standardize processes characterization, no specific criteria or standards regarding sterility biosafety have been established so far. This regulatory gap safety, sterilization, regulation has hampered clinical translation of decellularized scaffolds. In this review, we identify essential safe use products from both human animal sources. These include efficacy, levels chemical residue, preservation composition physical characteristics, aseptic processing assure sterility. Furthermore, explore key considerations advancing into practice, focusing on frameworks safety requirements. Addressing these challenges crucial minimizing risks adverse reactions infection transmission, thereby accelerating adoption tissue-engineered products. review aims provide foundation establishing robust guidelines, effective integration medicine applications.

Language: Английский

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Decellularized ECM hydrogels: prior use considerations, applications, and opportunities in tissue engineering and biofabrication

Biomaterials Science, Journal Year: 2022, Volume and Issue: 11(2), P. 400 - 431

Published: Dec. 2, 2022

Decellularized extracellular matrix hydrogels are tissue-derived materials that with proper processing can be used for tissue engineering applications and to build microenvironments in vitro . Figure was partly created Servier Medical Art.

Language: Английский

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The engineering and application of extracellular matrix hydrogels: a review

Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(11), P. 3784 - 3799

Published: Jan. 1, 2023

The engineering and appliccations of ECM hydrogels.

Language: Английский

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Tumor decellularization reveals proteomic and mechanical characteristics of the extracellular matrix of primary liver cancer

Biomaterials Advances, Journal Year: 2023, Volume and Issue: 146, P. 213289 - 213289

Published: Jan. 18, 2023

Tumor initiation and progression are critically dependent on interaction of cancer cells with their cellular extracellular microenvironment. Alterations in the composition, integrity, mechanical properties matrix (ECM) dictate tumor processes including cell proliferation, migration, invasion. Also primary liver cancer, consisting hepatocellular carcinoma (HCC) cholangiocarcinoma (CCA), dysregulation environment by fibrosis desmoplasia is pertinent. Yet, exact changes occurring ECM remain uncharacterized underlying tumor-promoting mechanisms largely unknown. Herein, an integrative molecular approach used to extensively characterize HCC CCA tumors utilizing optimized decellularization technique. We identified a myriad proteins both adjacent tissue, uncovering distinct malignancy-related signatures. The resolution this unveiled additional ECM-related compared large transcriptomic datasets. differences protein composition resulted divergent macro- micro-scale that tumor-type specific. Furthermore, decellularized was employed create tumor-specific hydrogel supports patient-derived organoids, which provides new avenue for personalized medicine applications. Taken together, study contributes better understanding alterations stiffness, collagen alignment occur during development.

Language: Английский

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