3D‐Printed Tissue‐Specific Nanospike‐Based Adhesive Materials for Time‐Regulated Synergistic Tumor Therapy and Tissue Regeneration In Vivo

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(48)

Published: June 26, 2024

Abstract The growing concerns regarding cancer recurrence, unpredictable bone deficiencies, and postoperative bacterial infections subsequent to the surgical removal of tumors have highlighted need for multifaceted scaffolds that afford tumor therapy, effective vascularized reconstruction. However, challenging trilemma has emerged in realm balance between achieving appropriate mechanical strength, ensuring biocompatibility, optimizing a degradation rate aligns with bone‐regenerative rate. Considering these challenges, innovative theragenerative platform is developed by utilizing 3D printing‐based nanospikes first time. This comprises tissue‐specific nanospiked hydroxyapatite decorated magnesium (nMg) adhesive DNA (aDNA). incorporation nMg within polylactic acid (PLA) matrix confers photothermal capabilities helps modulate properties improve biocompatibility platform. Simultaneously, immobilized aDNA contributed enhancement healing. These 3D‐printed tissue‐adhesive platforms exhibit superior offer controlled degradability. Moreover, they enable eradication bacteria osteosarcoma through hyperthermia promote angiogenesis osteogenesis, both vitro vivo. groundbreaking approach poised pave way fabrication design novel implantable biomaterials integrate therapeutic regenerative functions.

Language: Английский

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Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Regenerative Biomaterials, Journal Year: 2023, Volume and Issue: 11

Published: Dec. 1, 2023

Abstract Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering, clinical transformation of these remained a big challenge because difficulty simulating native organs/tissues’ microenvironment. As kind natural tissue-derived biomaterials, decellularized extracellular matrix (dECM)-based have gained attention due to their unique biomimetic properties, providing specific microenvironment suitable promoting cell proliferation, migration, attachment and regulating differentiation. The medical applications dECM-based addressed critical challenges, including poor mechanical strength insufficient stability. For reconstruction damaged tissues or organs, different types composite platforms been designed mimic microenvironment, by integrating with polymer or/and syntenic adding bioactive factors. In this review, we summarized research progress regenerative medicine, highlighting challenges future perspectives related application materials.

Language: Английский

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A Versatile Hydrogel with Antibacterial and Sequential Drug-Releasing Capability for the Programmable Healing of Infectious Keratitis

ACS Nano, Journal Year: 2023, Volume and Issue: 17(23), P. 24055 - 24069

Published: Dec. 4, 2023

Hydrogels have attracted tremendous attention as favorable corneal substitutes for treating severe infectious keratitis (IK). However, current hydrogel-based were majorly designed to promote the single stage of regeneration, which falls short in meeting clinical management needs IK including multiple phases wound healing. Herein, we introduce a versatile hybrid hydrogel (SQPV) composed silk fibroin and chitosan, exhibits spatiotemporal properties drug release. The SQPV is fabricated by incorporating verteporfin-loaded poly(lactic-co-glycolic)-polyethylene glycol-o-nitrobenzene micelles into network, formed from methacrylate glycidyl functionalized quaternized chitosan containing polydeoxyribonucleotide. This double network approach results material with exceptional anti-inflammatory, antibacterial, proliferative stimulation tissue remodeling regulation capabilities. Furthermore, showcases mechanical strength transparency akin those native cornea. Extensive vitro vivo studies validate SQPV's ability effectively eliminate residual bacteria, mitigate inflammation, foster regeneration epithelium stroma, prevent scarring, ultimately expedite In summary, SF/CS-based may represent promising substitute comprehensive repair IK.

Language: Английский

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Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 521 - 543

Published: May 30, 2024

Spinal cord injury (SCI) often results in irreversible loss of sensory and motor functions, most SCIs are incurable with current medical practice. One the hardest challenges treating SCI is development a dysfunctional pathological microenvironment, which mainly comprises excessive inflammation, deposition inhibitory molecules, neurotrophic factor deprivation, glial scar formation, imbalance vascular function. To overcome this challenge, implantation functional biomaterials at site has been regarded as potential treatment for modulating microenvironment to support axon regeneration, remyelination site, recovery after SCI. This review summarizes characteristics recent advances well technologies used modulate inflammatory regulate reshape revascularization microenvironment. Moreover, technological limitations, challenges, future prospects promote efficient repair also discussed. will aid further understanding

Language: Английский

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C-phycocyanin and quaternized chitosan based antibiotic-free hydrogels with antioxidant and antibacterial activity for wound healing

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140647 - 140647

Published: Feb. 1, 2025

Language: Английский

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Recent advances in extracellular vesicle engineering and its applications to regenerative medicine

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Extracellular vesicles (EVs) are nanosized particles that released from cells and reflect the characteristics of mother cell. Recently, EVs have been used in several types studies across many different fields. In field EV research, multiple cell culture isolation techniques highlighted importance. Various strategies, including exclusive component media, three-dimensional (3D) cultures, hypoxic conditions, proposed for to control function EVs. Ultracentrifugation, ultrafiltration, precipitation, tangential flow filtration (TFF) utilized isolation. Although isolated their own functionalities, researchers trying functionalize by applying various engineering approaches. Gene editing, exogenous, endogenous, hybridization methods four well-known functionalization strategies. engineered through these processes has applied regenerative medicine, kidney diseases, osteoarthritis, rheumatoid arthritis, nervous system-related others. this review, it was focused on approaches applications medicine.

Language: Английский

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Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury

Macromolecular Bioscience, Journal Year: 2024, Volume and Issue: 24(5)

Published: Jan. 15, 2024

Abstract Spinal cord injuries are very common worldwide, leading to permanent nerve function loss with devastating effects in the affected patients. The challenges and inadequate results current clinical treatments scientists innovative neural regenerative research. Advances nanoscience tissue engineering have opened new avenues for spinal injury (SCI) treatment. In order designed guidance conduit (NGC) be functionally useful, it must ideal scaffold properties topographic features that promote linear orientation of damaged axons. this study, is aimed develop channeled polycaprolactone (PCL)/Poly‐D,L‐lactic‐co‐glycolic acid (PLGA) hybrid film scaffolds, modify their surfaces by IKVAV pentapeptide/gold nanoparticles (AuNPs) or polypyrrole (PPy) investigate behavior motor neurons on vitro under static/bioreactor conditions. Their potential regeneration after implantation into rat SCI shaping scaffolds modified factors a tubular form also examined. It shown groups decorated AuNPs highly neurite bioreactor conditions developed optimal NGC (PCL/PLGA G1‐IKVAV/BDNF/NGF‐AuNP 50 ) regenerates SCI. indicate can an candidate regeneration.

Language: Английский

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3D hydrogel microfibers promote the differentiation of encapsulated neural stem cells and facilitate neuron protection and axon regrowth after complete transactional spinal cord injury

Biofabrication, Journal Year: 2024, Volume and Issue: 16(3), P. 035015 - 035015

Published: April 2, 2024

Spinal cord injury (SCI) can cause permanent impairment to motor or sensory functions. Pre-cultured neural stem cell (NSC) hydrogel scaffolds have emerged as a promising approach treat SCI by promoting anti-inflammatory effects, axon regrowth, and function restoration. Here, in this study, we performed coaxial extrusion process fabricate core-shell microfiber with high NSC density the core portion. Oxidized hyaluronic acid, carboxymethyl chitosan, matrigel blend were used matrix for growth facilitate fabrication process. During thein vitrodifferentiation culture, it was found that microfibers could differentiate into neurons astrocytes higher efficiency compared cultured petri dishes. Furthermore, duringin vivotransplantation, coated polylactic acid nanosheets electrospinning reinforcement. The nanofibers exhibited effect lesion cavity filling rate control group. Meanwhile, more neuron- oligodendrocyte-like cells visualized at epicenter. Finally, regrowth across whole site observed, demonstrating guide renascent regrowth. Experiment results indicate is bioactive treatment complete superior outcomes.

Language: Английский

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A N‐Cadherin Nano‐Antagonist Hydrogel Enhances Recovery From Spinal Cord Injury by Impeding Glial Scarring

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: July 30, 2024

Abstract The role of glial scars in the pathophysiology spinal cord injury (SCI) is widely recognized, as they pose physical barriers against axonal regeneration and persistent chronic inflammation by releasing cytotoxic agents, thereby impeding nerve repair. Consequently, preventing scarring has emerged an important therapeutic objective SCI management. Following SCI, astrocytes undergo a phenotypic transition into scar‐forming astrocytes, which critically depends on activation inflammatory responses integrin‐N‐cadherin pathway. To explore improved treatment, nano‐antagonist hydrogel (Nano‐ant Gel), comprising N‐cadherin nano‐antagonists polyphenol designed to inhibit mitigating response modulating astrocyte behavior, facilitating cord‐injury repair, developed characterized. exhibits notable anti‐inflammatory properties, specific calcium ion‐adsorption capabilities, antagonistic effects N‐cadherin, effectively formation aggregation astrocytes. Its efficacy comprehensively assessed using model contusive with it inhibits scar promotes regeneration. Notably, Nano‐ant Gel significantly improves locomotor functions mice suggesting that represents promising approach for treating condition.

Language: Английский

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Insights into Advances and Applications of Biomaterials for Nerve Tissue Injuries and Neurodegenerative Disorders

Macromolecular Bioscience, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Abstract The incidence of nerve tissue injuries, such as peripheral injury, spinal cord traumatic brain and various neurodegenerative diseases (NDs), is continuously increasing because stress, physical chemical trauma, the aging population worldwide. Restoration damaged nervous system challenging its structural functional complexity limited regenerative ability. Additionally, there no cure available for NDs except medications that provide symptomatic relief. Stem cells offer an alternative approach promoting damage repair, but their efficacy by a compromised survival rate neurogenesis process. To address these challenges, neural engineering has emerged promising strategy in which stem are seeded or encapsulated within suitable biomaterial construct, cell neurogenesis. Numerous biomaterials utilized to create different types constructs this purpose. Researchers trying develop ideal scaffolds combine biomaterials, cells, molecules exactly mimic biological mechanical properties achieve recovery associated with neurological dysfunction. This review focuses on exploring development applications potential use diagnosis, therapy, regeneration, treatment disorders.

Language: Английский

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