RETRACTED ARTICLE: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Journal of Biomedical Science, Journal Year: 2020, Volume and Issue: 27(1)

Published: Jan. 3, 2020

Abstract Background Circular RNAs (circRNAs) represent a class of non-coding (ncRNAs) which are widely expressed in mammals and tissue-specific, some could act as critical regulators the atherogenesis cerebrovascular disease. However, underlying mechanisms by circRNA regulates ectopic phenotype endothelial cells (ECs) atherosclerosis remain largely elusive. Methods CCK-8, transwell, wound healing Matrigel assays were used to assess cell viability, migration tube formation. QRT-qPCR Immunoblotting examine targeted gene expression different groups. The binding sites miR-370-3p (miR-370) with TGFβR2 or hsa_circ_0003204 (circ_0003204) predicted using series bioinformatic tools, validated dual luciferase assay RNA immunoprecipitation (RIP) assay. localization circ_0003204 miR-370 ECs investigated fluorescence situ hybridization (FISH). Gene function pathways enriched through Metascape set enrichment analysis (GSEA). association extracellular vesicles (EVs) clinical characteristics patients multiple statistical analysis. Results Circ_0003204, mainly located cytoplasm human aorta (HAECs), was upregulated ox-LDL-induced HAECs. Functionally, inhibited proliferation, formation HAECs exposed ox-LDL. Mechanically, promote protein its downstream phosph-SMAD3 sponging miR-370, 3′ untranslated region (UTR) TGFβR2. Furthermore, plasma EVs cerebral atherosclerosis, represented potential biomarker for diangnosis prognosis atherogenesis. Conclusions Circ_0003204 novel stimulator inactivation atherosclerosis.

Language: Английский

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CircRNA‑0044073 is upregulated in atherosclerosis and increases the proliferation and invasion of cells by targeting miR‑107

Molecular Medicine Reports, Journal Year: 2019, Volume and Issue: unknown

Published: March 6, 2019

Circular RNAs (circRNAs) are endogenous non‑coding implicated in atherosclerosis. The aim of the present study was to explore function circRNA‑0044073 Reverse transcription quantitative polymerase chain reaction assays were used measure expression levels circRNA‑0044073, microRNA (miRNA/miR)‑107, janus kinase 1 (JAK1), signal transducer and activator 3 (STAT3), B‑cell lymphoma 2 (Bcl‑2) v‑myc avian myelocytomatosis viral oncogene homolog (c‑myc) blood cells from patients with RNA pull‑down luciferase reporter then determine association between circRNA miR expression, gene respectively. Matrigel invasion assay flow cytometry analyze cell cycle. Western blot analysis ELISA evaluate proteins. It identified that upregulated miR‑107 downregulated atherosclerotic cells. Overexpression promoted proliferation human vascular smooth muscle (HUVSMCs) endothelial (HUVECs), while overexpression inhibited their proliferation. In addition, suppressed via a sponge mechanism. Lipopolysaccharide (LPS) affected HUVSMCs HUVECs, also resulted changes levels. CircRNA‑0044073 HUVECs spite opposite effect observed LPS treatment. JAK/STAT signaling pathway activated favored activation inflammation HUVECs. These data indicate is atherosclerosis promotes by targeting activating pathway, potentially offering target for novel treatment strategies against

Language: Английский

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Microarray profiles reveal that circular RNA hsa_circ_0007385 functions as an oncogene in non-small cell lung cancer tumorigenesis

Journal of Cancer Research and Clinical Oncology, Journal Year: 2018, Volume and Issue: 144(4), P. 667 - 674

Published: Jan. 24, 2018

Language: Английский

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DWNN-RLS: regularized least squares method for predicting circRNA-disease associations

BMC Bioinformatics, Journal Year: 2018, Volume and Issue: 19(S19)

Published: Dec. 1, 2018

Many evidences have demonstrated that circRNAs (circular RNA) play important roles in controlling gene expression of human, mouse and nematode. More importantly, are also involved many diseases through fine tuning post-transcriptional by sequestering the miRNAs which associate with diseases. Therefore, identifying circRNA-disease associations is very appealing to comprehensively understand mechanism, treatment diagnose diseases, yet challenging. As complex mechanism between wet-lab experiments expensive time-consuming discover novel associations. it dire need employ computational methods associations.In this study, we develop a method (DWNN-RLS) predict based on Regularized Least Squares Kronecker product kernel. The similarity computed from Gaussian Interaction Profile(GIP) known In addition, integrated mean GIP sematic direct acyclic graph (DAG) representation kernels pairs constructed DWNN (decreasing weight k-nearest neighbor) adopted calculate initial relational score for new kernel regularised least squares approach used We adopt 5-fold cross validation (5CV), 10-fold (10CV) leave one out (LOOCV) assess prediction performance our method, compare other six competing (RLS-avg, RLS-Kron, NetLapRLS, KATZ, NBI, WP).The experiment results show DWNN-RLS reaches AUC values 0.8854, 0.9205 0.9701 5CV, 10CV LOOCV, respectively, illustrates superior RLS-avg, WP. case studies an effective

Language: Английский

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Insights into the regulatory role of circRNA in angiogenesis and clinical implications

Atherosclerosis, Journal Year: 2020, Volume and Issue: 298, P. 14 - 26

Published: Feb. 25, 2020

Language: Английский

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IFN regulatory Factor-1 induced macrophage pyroptosis by modulating m6A modification of circ_0029589 in patients with acute coronary syndrome

International Immunopharmacology, Journal Year: 2020, Volume and Issue: 86, P. 106800 - 106800

Published: July 13, 2020

Language: Английский

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Circular RNAs are a novel type of non-coding RNAs in ROS regulation, cardiovascular metabolic inflammations and cancers

Pharmacology & Therapeutics, Journal Year: 2020, Volume and Issue: 220, P. 107715 - 107715

Published: Oct. 24, 2020

Language: Английский

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Circular RNAs in the pathogenesis of atherosclerosis

Life Sciences, Journal Year: 2020, Volume and Issue: 255, P. 117837 - 117837

Published: May 22, 2020

Language: Английский

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Circular RNAs in the tumour microenvironment

Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)

Published: Jan. 14, 2020

Abstract Background Circular RNAs (circRNAs) are a new class of endogenous non-coding (ncRNAs) widely expressed in eukaryotic cells. Mounting evidence has highlighted circRNAs as critical regulators various tumours. More importantly, have been revealed to recruit and reprogram key components involved the tumour microenvironment (TME), mediate signaling pathways, thus affecting tumourigenesis, angiogenesis, immune response, metastatic progression. Main body abstract In this review, we briefly introduce biogenesis, characteristics classification circRNAs, describe mechanistic models circRNAs. Further, provide first systematic overview interplay between cellular/non-cellular counterparts TME highlight potential prospective biomarkers or targets cancer clinics. Finally, discuss biological mechanisms through which drive development resistance, revealing mystery drug resistance Short conclusion Deep understanding emerging role their involvements may therapeutic for patients. The combined targeting co-activated achieve higher efficiency become mode therapy future.

Language: Английский

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Kaempferol and atherosclerosis: From mechanism to medicine

Critical Reviews in Food Science and Nutrition, Journal Year: 2022, Volume and Issue: 64(8), P. 2157 - 2175

Published: Sept. 13, 2022

Natural products possess pleiotropic cardiovascular protective effects owing to their anti-oxidation, anti-inflammation and anti-thrombotic properties. Kaempferol, (3,5,7-trihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one), is a kind of naturally occurring flavonoid existing in many common fruits vegetables (e.g., onions, broccoli, strawberries grapes) particularly traditional Chinese medicine as exemplified by Ginkgo biloba. Epidemiological, preclinical clinical studies have revealed an inverse association between the consumption kaempferol-containing foods medicines risk developing diseases. Numerous translational experimental animal models cultured cells demonstrated wide range pharmacological activities kaempferol. In this article, we reviewed antioxidant, anti-inflammatory cardio-protective kaempferol elucidated potential molecular basis therapeutic capacity focusing on its anti-atherosclerotic effects. Overall, review presents health benefits plants reflects possible drug candidate prevent treat atherosclerosis, underlying pathology most

Language: Английский

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