Journal of Molecular Neuroscience, Journal Year: 2025, Volume and Issue: 75(1)
Published: Jan. 22, 2025
Language: Английский
Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)
Published: Jan. 14, 2022
Gliomas are the most common malignant primary brain tumours with a highly immunosuppressive tumour microenvironment (TME) and poor prognosis. Circular RNAs (circRNA), newly found type of endogenous noncoding RNA, characterized by high stability, abundance, conservation, have been shown to play an important role in pathophysiological processes TME remodelling various tumours.CircRNA sequencing analysis was performed explore circRNA expression profiles normal glioma tissues. The biological function novel circRNA, namely, circNEIL3, development confirmed both vitro vivo. Mechanistically, RNA pull-down, mass spectrum, immunoprecipitation (RIP), luciferase reporter, co-immunoprecipitation assays were conducted.We identified which could be cyclized EWS RNA-binding protein 1(EWSR1), upregulated tissues correlate positively progression. Functionally, we that circNEIL3 promotes tumorigenesis carcinogenic progression stabilizes IGF2BP3 (insulin-like growth factor 2 mRNA binding 3) protein, known oncogenic preventing HECTD4-mediated ubiquitination. Moreover, overexpression cells drives macrophage infiltration into (TME). Finally, is packaged exosomes hnRNPA2B1 transmitted infiltrated associated macrophages (TAMs), enabling them acquire properties stabilizing turn promoting progression.This work reveals plays nonnegligible multifaceted gliomagenesis, tumour-promoting phenotypes polarization, highlighting potential prognostic biomarker therapeutic target glioma.
Language: Английский
Citations
216
Molecular Cancer, Journal Year: 2021, Volume and Issue: 20(1)
Published: Feb. 3, 2021
Colorectal cancer (CRC) is a common hereditary tumor that often fatal. Its pathogenesis involves multiple genes, including circular RNAs (circRNAs). Notably, circRNAs constitute new class of noncoding (ncRNAs) with covalently closed loop structure and have been characterized as stable, conserved molecules are abundantly expressed in tissue/development-specific patterns eukaryotes. Based on accumulating evidence, aberrantly CRC tissues, cells, exosomes, blood from patients CRC. Moreover, numerous identified either oncogenes or suppressors mediate tumorigenesis, metastasis chemoradiation resistance Although the regulatory mechanisms circRNA biogenesis functions remain fairly elusive, interesting results obtained studies investigating In particular, expression comprehensively modulated by factors, such splicing transcription specific enzymes cis-acting elements. More importantly, exert pivotal effects through various mechanisms, acting miRNA sponges decoys, interacting RNA binding proteins, even translating functional peptides. Finally, may serve promising diagnostic prognostic biomarkers potential therapeutic targets clinical practice this review, we discuss dysregulation, significance further molecular which their how regulated. hope to reveal initiation progression highlight future perspectives strategies targeting research.
Language: Английский
Citations
141
Journal of Experimental & Clinical Cancer Research, Journal Year: 2024, Volume and Issue: 43(1)
Published: Jan. 20, 2024
Abstract Signal transducer and activator of transcription 3 (STAT3) is a transcriptional factor involved in almost all cancer hallmark features including tumor proliferation, metastasis, angiogenesis, immunosuppression, inflammation, metabolism reprogramming, drug resistance, stemness. Therefore, STAT3 has become promising therapeutic target wide range cancers. This review focuses on the up-to-date knowledge signaling cancer. We summarize both positive negative modulators together with hallmarks involving activities regulated by highlight its extremely sophisticated regulation immunosuppression microenvironment metabolic reprogramming. Direct indirect inhibitors preclinical clinical studies also have been summarized discussed. Additionally, we propose new strategies targeting STAT3-based combinations established chemotherapy, targeted therapy, immunotherapy combination therapy. These efforts may provide perspectives for therapy
Language: Английский
Citations
67
Molecular Cancer, Journal Year: 2020, Volume and Issue: 19(1)
Published: Oct. 14, 2020
Abstract Renal cell carcinoma (RCC) is the most common malignant kidney tumor and has a high incidence rate. Circular RNAs (circRNAs) are noncoding with widespread distribution diverse cellular functions. They highly stable have organ- tissue-specific expression patterns. CircRNAs essential functions as microRNA sponges, RNA-binding protein- transcriptional regulators, protein translation templates. Recent reports shown that circRNAs abnormally expressed in RCC act important regulators of carcinogenesis progression. Moreover, emerged potential biomarkers for diagnosis prognosis targets developing new treatments. However, further studies needed to better understand RCC. In this review, we summarize discuss recent research progress on RCC-associated circRNAs, focus their targeted therapy.
Language: Английский
Citations
81
Theranostics, Journal Year: 2021, Volume and Issue: 12(1), P. 87 - 104
Published: Nov. 10, 2021
Exosomes are multifunctional regulators of intercellular communication by carrying various messages under both physiological and pathological status cancer patients. Accumulating studies have identified the presence circular RNAs (circRNAs) in exosomes with crucial regulatory roles diverse pathophysiological processes. Exosomal circRNAs derived from donor cells can modulate crosstalk recipient locally or remotely to enhance development propagation, play tumor microenvironment (TME), leading significant enhancement immunity, metabolism, angiogenesis, drug resistance, epithelial mesenchymal transition (EMT), invasion metastasis. In this review, we describe advances exosomal their modulating hallmarks, especially those TME. Moreover, clinical application potential diagnosis therapy highlighted, bridging gap between basic knowledge practice.
Language: Английский
Citations
80
Molecular Therapy — Nucleic Acids, Journal Year: 2021, Volume and Issue: 27, P. 50 - 72
Published: Nov. 19, 2021
Lung cancer (LC) is the leading cause of cancer-related deaths worldwide, with high morbidity and mortality. Non-small cell lung (NSCLC) a major pathological type LC accounts for more than 80% all cases. Circular RNAs (circRNAs) are large class non-coding (ncRNAs) covalently closed-loop structures, abundance, tissue-specific expression patterns. They participate in various pathophysiological processes by regulating complex gene networks involved proliferation, apoptosis, migration, epithelial-to-mesenchymal transition (EMT), as well metastasis. A growing number studies have revealed that dysregulation circRNAs contributes to many aspects progression, such its occurrence, metastasis, recurrence, suggesting their great potential efficient specific biomarkers diagnosis, prognosis, therapeutic targeting NSCLC. In this review, we systematically elucidate characteristics, biogenesis, functions focus on molecular mechanisms NSCLC progression. Moreover, highlight clinical implications treatment.
Language: Английский
Citations
68
Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)
Published: Feb. 16, 2023
Transfer RNAs (tRNAs) are a class of non-coding responsible for amino acid translocation during protein synthesis and ubiquitously found in organisms. With certain modifications under specific conditions, tRNAs can be sheared fragmented into small RNAs, also known as tRNA-derived (tDRs). the development high-throughput sequencing technologies bioinformatic strategies, more tDRs have been identified their functions organisms characterized. tRNA it derived tDRs, shown to essential not only transcription translation, but regulating cell proliferation, apoptosis, metastasis, immunity. Aberrant expression is associated with wide range human diseases, especially tumorigenesis tumor progression. The microenvironment (TME) complex ecosystem consisting various cellular cell-free components that mutually compatible tumor. It has regulate TME by cancer stem cells, immunity, energy metabolism, epithelial mesenchymal transition, extracellular matrix remodeling, playing pro-tumor or suppressor role. In this review, biogenesis, classification, function well effects on clinical application prospects will summarized discussed based up date available knowledge.
Language: Английский
Citations
33
MedComm, Journal Year: 2024, Volume and Issue: 5(8)
Published: July 29, 2024
Abstract mRNA vaccines are regarded as a highly promising avenue for next‐generation cancer therapy. Nevertheless, the intricacy of production, inherent instability, and low expression persistence linear significantly restrict their extensive utilization. Circular RNAs (circRNAs) offer novel solution to these limitations due efficient protein ability, which can be rapidly generated in vitro without need extra modifications. Here, we present neoantigen vaccine based on circRNA that induces potent anti‐tumor immune response by expressing hepatocellular carcinoma‐specific tumor neoantigens. By cyclizing linearRNA molecules, were able enhance stability RNA form stable molecules with capacity sustained expression. We confirmed neoantigen‐encoded promote dendritic cell (DC) activation DC‐induced T‐cell vitro, thereby enhancing killing cells. Encapsulating within lipid nanoparticles vivo has enabled creation platform. This platform demonstrates superior treatment prevention various murine models, eliciting robust response. Our offers new options application prospects immunotherapy solid tumors.
Language: Английский
Citations
17
Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)
Published: Aug. 21, 2024
Circular RNAs (circRNAs) are unique noncoding that have a closed and stable loop structure generated through backsplicing. Due to their conservation, stability tissue specificity, circRNAs can potentially be used as diagnostic indicators therapeutic targets for certain tumors. Many studies shown act microRNA (miRNA) sponges, engage in interactions with proteins translation templates regulate gene expression signal transduction, thereby participating the occurrence development of variety malignant Immunotherapy has revolutionized treatment cancer. Early researches indicated involved regulating tumor immune microenvironment antitumor immunity. CircRNAs may potential important increasing sensitivity immunotherapy expanding population patients who benefit from cancer immunotherapy. However, few investigated correlation between In this review, we summarize current on regulation different mechanisms value efficacy goal providing new
Language: Английский
Citations
11
Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)
Published: May 7, 2024
Epidermal growth factor receptor (EGFR)-targeted therapy is an important treatment for RAS wild-type metastatic colorectal cancer (mCRC), but the resistance mechanism remains unclear. Here, differential expression of circRNAs between Cetuximab sensitive and resistant cell lines was analyzed using whole-transcriptome sequencing. We identified that circHIF1A significantly higher in LIM1215-R than LIM1215. When treated with Cetuximab, downregulation level weakened proliferation clonal formation ability LIM1215-R, caused more cells to enter G0-G1 phase, reduced basal respiration, ATP production, maximal as well glycolytic capacity reserve. The response rate prognosis circHIF1A-positive patients were inferior those negative patients. Mechanistically, can upregulate hypoxia-inducible 1 A (HIF1A) by competitively binding miR-361-5p, inducing overexpression enzymes such glucose transporter (GLUT1) lactate dehydrogenase (LDHA). In a xenograft model, inhibition increased sensitivity treatment. conclusion, promote HIF1α-mediated glycometabolism alteration induce CRC. It has potential become screening indicator beneficial population mCRC new therapeutic target enhancing efficacy.
Language: Английский
Citations
10