Emerging functions and clinical applications of exosomal microRNAs in diseases DOI Creative Commons
Soudeh Ghafouri‐Fard, Hamed Shoorei, Peixin Dong

et al.

Non-coding RNA Research, Journal Year: 2023, Volume and Issue: 8(3), P. 350 - 362

Published: May 9, 2023

Exosomes are an important group of extracellular vesicles that transfer several kinds biomolecules and facilitate cell-cell communication. The content exosomes, particularly the amounts microRNA (miRNAs) inside these vesicles, demonstrates a disease-specific pattern reflecting pathogenic processes may be employed as diagnostic prognostic marker. miRNAs enter recipient cells through exosomes generate RISC complex can cause degradation target mRNAs or block translation their corresponding proteins. Therefore, exosome-derived constitute mechanism gene regulation in cells. miRNA used tool detection diverse disorders, cancers. This research field has situation cancer diagnosis. In addition, exosomal microRNAs offer great deal promise treatment human disorders. However, there still certain challenges to resolved. most follow: should standardized, miRNAs-associated studies conducted large number clinical samples, experiment settings criteria consistent across different labs. goal this article is present overview effects on variety diseases, including gastrointestinal, pulmonary, neurological, cardiovascular with particular emphasis malignancies.

Language: Английский

Unveiling the connection: Long-chain non-coding RNAs and critical signaling pathways in breast cancer DOI
Riya Thapa, Obaid Afzal, Gaurav Gupta

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 249, P. 154736 - 154736

Published: Aug. 3, 2023

Language: Английский

Citations

79
Noncoding RNAs: Master Regulator of Fibroblast to Myofibroblast Transition in Fibrosis DOI Open Access
Huamin Zhang,

Yutong Zhou,

Dada Wen

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(2), P. 1801 - 1801

Published: Jan. 16, 2023

Myofibroblasts escape apoptosis and proliferate abnormally under pathological conditions, especially fibrosis; they synthesize secrete a large amount of extracellular matrix (ECM), such as α-SMA collagen, which leads to the distortion organ parenchyma structure, an imbalance in collagen deposition degradation, replacement parenchymal cells by fibrous connective tissues. Fibroblast myofibroblast transition (FMT) is considered be main source myofibroblasts. Therefore, it crucial explore influencing factors regulating process FMT for prevention, treatment, diagnosis FMT-related diseases. In recent years, non-coding RNAs, including microRNA, long circular have attracted extensive attention from scientists due their powerful regulatory functions, been found play vital role FMT. this review, we summarized ncRNAs regulate during fibrosis that mainly regulated signaling pathways, TGF-β/Smad, MAPK/P38/ERK/JNK, PI3K/AKT, WNT/β-catenin. Furthermore, expression downstream transcription can promoted or inhibited, indicating potential new therapeutic target

Language: Английский

Citations

27
GAS5 lncRNA: A biomarker and therapeutic target in breast cancer DOI
Lakshmi Thangavelu, Ehssan Moglad, Gaurav Gupta

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 260, P. 155424 - 155424

Published: June 20, 2024

Language: Английский

Citations

11
Current insights into transcriptional role(s) for the nutraceutical Withania somnifera in inflammation and aging DOI Creative Commons

Praful Saha,

Saiprasad Ajgaonkar,

Dishant Maniar

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: May 3, 2024

The health-beneficial effects of nutraceuticals in various diseases have received enhanced attention recent years. Aging is a continuous process wherein physiological activity an individual declines over time and characterized by indefinite hallmarks which contribute toward aging-related comorbidities include many neurodegenerative diseases, cardiac problems, diabetes, bone-degeneration, cancer. Cellular senescence homeostatic biological that has important function driving aging. Currently, growing body evidence substantiates the connection between epigenetic modifications aging process, along with diseases. These are now being recognized as promising targets for emerging therapeutic interventions. Considering almost all processes modulated RNAs, numerous RNA-binding proteins been found to be linked age-related complexities. studies shed light on ability nutraceutical Withania somnifera (Ashwagandha) influence RNA expression, stability, processing, offering insights into its mechanisms action. By targeting RNA-related pathways, may exhibit ameliorating age-associated molecular changes, gene expression signaling networks. This review summarizes potential role modulating RNA-level changes associated aging, encompassing both vitro vivo studies. Taken together, putative role(s) modulation key RNAs will provide understanding facilitate development preventive strategies employing healthy

Language: Английский

Citations

9
Identification and characterization of lncRNA-miRNA-mRNA tripartite network of sulfur mustard exposed patients DOI
Masoud Arabfard,

Shahram Parvin,

Mostafa Ghanei

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 149, P. 114204 - 114204

Published: Feb. 6, 2025

Language: Английский

Citations

1
Function of miRNA-145–5p in the pathogenesis of human disorders DOI
Sepideh Kadkhoda, Soudeh Ghafouri‐Fard

Pathology - Research and Practice, Journal Year: 2022, Volume and Issue: 231, P. 153780 - 153780

Published: Jan. 25, 2022

Language: Английский

Citations

36
Novel Therapies for the Treatment of Cardiac Fibrosis Following Myocardial Infarction DOI Creative Commons
Kamila Raziyeva,

Yevgeniy Kim,

Zharylkasyn Zharkinbekov

et al.

Biomedicines, Journal Year: 2022, Volume and Issue: 10(9), P. 2178 - 2178

Published: Sept. 2, 2022

Cardiac fibrosis is a common pathological consequence of most myocardial diseases. It associated with the excessive accumulation extracellular matrix proteins as well fibroblast differentiation into myofibroblasts in cardiac interstitium. This structural remodeling often results dysfunctions such arrhythmias and impaired systolic function patients heart conditions, ultimately leading to failure death. An understanding precise mechanisms still limited due numerous signaling pathways, cells, mediators involved process. review article will focus on pathophysiological processes development fibrosis. In addition, it summarize novel strategies for anti-fibrotic therapies epigenetic modifications, miRNAs, CRISPR technologies various medications cellular animal models.

Language: Английский

Citations

34
Exosomes from adipose-derived mesenchymal stem cell improve diabetic wound healing and inhibit fibrosis via miR-128-1-5p/TGF-β1/Smad axis DOI
Qiu Liang, D. Zhou, Xiuyu Ge

et al.

Molecular and Cellular Endocrinology, Journal Year: 2024, Volume and Issue: 588, P. 112213 - 112213

Published: March 29, 2024

Language: Английский

Citations

8
Epithelium-derived exosomes promote silica nanoparticles-induced pulmonary fibroblast activation and collagen deposition via modulating fibrotic signaling pathways and their epigenetic regulations DOI Creative Commons
Yan Li, Hailin Xu,

Ying Wang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 12, 2024

Abstract Background In the context of increasing exposure to silica nanoparticles (SiNPs) and ensuing respiratory health risks, emerging evidence has suggested that SiNPs can cause a series pathological lung injuries, including fibrotic lesions. However, underlying mediators in fibrogenesis caused by have not yet been elucidated. Results The vivo investigation verified long-term inhalation induced fibroblast activation collagen deposition rat lungs . vitro, uptake exosomes derived from SiNPs-stimulated epithelial cells (BEAS-2B) fibroblasts (MRC-5) enhanced its proliferation, adhesion, activation. particular, mechanistic revealed stimulated an increase epithelium-secreted exosomal miR-494-3p thereby disrupted TGF-β/BMPR2/Smad pathway via targeting bone morphogenetic protein receptor 2 (BMPR2), ultimately resulting deposition. Conversely, inhibitor exosomes, GW4869, abolish induction upregulated MRC-5 SiNPs-treated supernatants BEAS-2B. Besides, inhibiting or overexpression BMPR2 could ameliorate interfering with pathway. Conclusions Our data pulmonary epithelium-derived serve essential role upon stimuli, attributing modulate Hence, strategies be new avenue developing therapeutics against injury elicited SiNPs. Graphical

Language: Английский

Citations

8
Exosomal miRNA-146a-5p Derived from Senescent Hepatocellular Carcinoma Cells Promotes Aging and Inhibits Aerobic Glycolysis in Liver Cells via Targeting IRF7 DOI Creative Commons
Sijia Yang, Ang Li,

Lihong Lv

et al.

Journal of Cancer, Journal Year: 2024, Volume and Issue: 15(14), P. 4448 - 4466

Published: Jan. 1, 2024

Hepatocellular carcinoma (HCC) is a major global health challenge.Chemotherapy can cause HCC cells to become senescent.Senescent play an important role in inhibiting or promoting cancer by producing extracellular vesicles with senescence-associated secretory phenotype (EV-SASP).miRNA be strongly upregulated EV-SASP during the aging process and substantially alter phenotypic characteristics of cells.MiRNA microarray analysis revealed that miRNA-146a-5p was highly expressed oxaliplatin-and H2O2-induced senescent Huh7 cells, RT-PCR confirmed its significant upregulation exosomes.The transcriptome sequencing results overexpressing suggested could regulate cell glycolysis.Subsequently, dual luciferase assay used verify whether interact IRF7 promote aging.The key functions aerobic glycolysis liver were determined through experiments analyzing glucose uptake, lactate production, oxygen consumption rate (OCR) proton efflux (PER).Subsequently, regulatory effect on glycolytic gene PFKL reporter assays.The western blot experiment showed miR-146a-5p activate CHK2 p53 phosphorylated proteins targeting IRF7, upregulate p21 protein.Overexpression effectively inhibited function cells.Moreover, silencing glycolysis.MiR-146a-5p.MiR-146a-5p phosphorylation protein downstream activated upregulates expression p21.Our study exosomal produced inhibit proliferation activating CHK2/p53/p21 signaling way IRF7.

Language: Английский

Citations

7