Association between miRNA-145 and miRNA-155 expression in peripheral blood mononuclear cells of patients with multiple sclerosis: a case-control study DOI Creative Commons

Sepide Ali Ashrafi,

Milad Asadi, Dariush Shanehbandi

et al.

BMC Neurology, Journal Year: 2022, Volume and Issue: 22(1)

Published: Nov. 3, 2022

MicroRNAs (miR or miRNA) are short regulatory RNAs, which modulate post-transcriptional gene expression. Dysregulation of these molecules contributes to pathogenicity autoimmune disorders, such as multiple sclerosis (MS).This study was conducted investigate changed expression pattern miRNA-145 and miRNA-155 in MS.We collected blood samples 75 patients with relapsing-remitting MS healthy controls. Ficoll-Hypaque density gradient method used isolate peripheral mononuclear cells. Also, total RNA extracted subjected RT-PCR analysis. We the Mann-Whitney U test evaluate differences levels target miRNAs between groups.We found that (P = 0.012) 0.005) were partly reduced relapse-remitting comparison The had an area under curve (AUC) 0.621 0.01) AUC 0.625 0.008).Decreased development MS, while further large scale observational studies meta-analyses required.

Language: Английский

The enigmatic world of tear extracellular vesicles (EVs)–exploring their role in ocular health and beyond DOI Creative Commons
Anees Fatima, Shalini Sanyal,

Gaurab Kumar Jha

et al.

FEBS Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Extracellular vesicles (EVs) are released by all kind of cells into the extracellular space, where they shuttle parental cell‐derived molecular cargoes (DNA, RNA, proteins) to both adjacent and distant cells, influencing physiology target cells. Their specific cargo content abundance in liquid biopsies make them excellent candidates for biomarker studies. Indeed, EVs isolated from various body fluids, including blood, pleural fluid, urine, cerebrospinal saliva, milk, ascites, tears, have been recognized their potential as biomarkers diagnosis, monitoring treatment, predicting outcomes diseases. Increasing studies suggest that tears great promise a noninvasive biopsy source EVs. Our aim here is provide comprehensive review exploration reservoir contents, evaluating accessibility utility method. Additionally, tear cancers, ocular cancer, discussed. Finally, advantages challenges employing tear‐based disease ' s evaluated.

Language: Английский

Citations

1
Direct Extraction and Evaluation of Intraluminal Vesicles Inside a Single Cell DOI
Hiroki Ida, Takeshi Yoshida, Akichika Kumatani

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Because endogenous extracellular vesicles are involved in important physiological functions, various techniques have been developed for their isolation and evaluation. However, methods evaluating within cells limited. This study presents a technique the direct extraction evaluation of intraluminal (ILVs). combines scanning ion conductance microscopy, electrochemical syringes, confocal microscopy to extract specific structures living cell, achieving high spatial resolution accuracy at femtoliter scale. approach allowed collection CD63(+) from HEK293 CD63-pHluorin-RFP showed that RNA expression profiles were different those recovered cytosol isolated by ultracentrifuge. It also identified subset specifically containing hsa-miR-145-5p assessment local accumulation miRNAs cells. is expected become powerful tool contents ILVs

Language: Английский

Citations

1
LncRNA MEG3 Inhibits Tumor Progression by Modulating Macrophage Phenotypic Polarization via miR-145-5p/DAB2 Axis in Hepatocellular Carcinoma DOI Creative Commons
Qing Wei, Guoman Liu, Zihua Huang

et al.

Journal of Hepatocellular Carcinoma, Journal Year: 2023, Volume and Issue: Volume 10, P. 1019 - 1035

Published: July 1, 2023

Hepatocellular carcinoma (HCC) is the predominant histological type of primary liver cancer, which ranks sixth among most common human tumors. Tumor-associated macrophages (TAMs) are an important component tumor microenvironment (TME) and M2 macrophage polarization substantially contributes to growth metastasis. Long non-coding RNA (lncRNA) MEG3 was reported restrain HCC development. However, whether regulates phenotypic in remains unclear.Bone marrow derived (BMDMs) were treated with LPS/IFNγ IL4/IL13 induce M1 polarization, respectively. M2-polarized BMDMs simultaneously transfected adenovirus vector overexpressing (Adv-MEG3). Subsequently, cultured for 24 h serum-free medium, supernatants harvested as conditioned medium (CM). cell line Huh7 CM h. F4/80+CD68+ F4/80+CD206+ percentages M1-and calculated using flow cytometry. migration, invasion angiogenesis determined via Transwell assay tube formation experiment. Nude mice implanted cells Adv-MEG3-transfected M2-polarizd BMDMs, markers assessed. The binding between miR-145-5p or disabled-2 (DAB2) verified by luciferase reporter assay.MEG3 presented lower expression tissues than normal controls, low correlated poorer prognosis patients. enhanced during LPS/IFNγ-induced but reduced IL4/IL13-induced polarization. overexpression inhibited both mice. Mechanically, bound regulate DAB2 expression. Overexpressing suppressed polarization-induced metastasis upregulating vivo growth.LncRNA curbs development repressing miR-145-5p/DAB2 axis.

Language: Английский

Citations

19
High microRNA-145 plasma levels are associated with decreased risk of future incident venous thromboembolism: the HUNT study DOI
Vânia M. Morelli, Omri Snir, Kristian Hindberg

et al.

Blood, Journal Year: 2024, Volume and Issue: 143(17), P. 1773 - 1781

Published: Jan. 11, 2024

Language: Английский

Citations

8
Emerging roles and mechanisms of miR-206 in human disorders: a comprehensive review DOI Creative Commons
Sheyda Khalilian, Seyedeh Zahra Hosseini Imani, Soudeh Ghafouri‐Fard

et al.

Cancer Cell International, Journal Year: 2022, Volume and Issue: 22(1)

Published: Dec. 17, 2022

Abstract As a member of the miR-1 family, miR-206 is located between IL-17 and PKHD1 genes in human. This miRNA has been shown to be involved pathogenic processes variety human disorders including cancers, amyotrophic lateral sclerosis, Alzheimer’s disease, atherosclerosis, bronchopulmonary dysplasia, coronary artery chronic obstructive pulmonary epilepsy, nonalcoholic fatty liver Hirschsprung muscular dystrophies, arterial hypertension, sepsis ulcerative colitis. In current review, we summarize role both malignant non-malignant situations explain its possible therapeutic implications.

Language: Английский

Citations

24
Non-Coding RNAs in Tuberculosis Epidemiology: Platforms and Approaches for Investigating the Genome’s Dark Matter DOI Open Access
Ahmad Almatroudi

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(8), P. 4430 - 4430

Published: April 17, 2022

A growing amount of information about the different types, functions, and roles played by non-coding RNAs (ncRNAs) is becoming available, as more research done. ncRNAs have been identified potential therapeutic targets in treatment tuberculosis (TB), because they may be essential regulators gene network. ncRNA profiling sequencing has recently revealed significant dysregulation tuberculosis, primarily due to aberrant processes synthesis, including amplification, deletion, improper epigenetic regulation, or abnormal transcription. Despite fact that a role TB characteristics, detailed mechanisms behind these occurrences are still unknown. The dark matter genome can only explored through development cutting-edge bioinformatics molecular technologies. In this review, ncRNAs’ synthesis functions discussed detail, with an emphasis on tuberculosis. We also focus current platforms, experimental strategies, computational analyses explore TB. Finally, viewpoint presented key challenges novel techniques for future wide-ranging application ncRNAs.

Language: Английский

Citations

23
Inhibition of breast cancer cell growth and migration through siRNA-mediated modulation of circ_0009910/miR-145-5p/MUC1 axis DOI Creative Commons
Maryam Abtin,

Nahid Nafisi,

Asghar Hosseinzadeh

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(2), P. 367 - 375

Published: Jan. 26, 2024

Circular RNAs (circRNAs) characterize a novel kind of regulatory distinguished by great evolutionary conservation and constancy. Although their exact role in malignancies is not fully understood, they mainly work through specific axes. RNA/miRNA/mRNA axes affect the pathogenesis human cancers including breast cancer. We assessed expression function circ_0009910/miR-145-5p/MUC1 axis Breast Cancer tissues MCF-7 cells. Expression levels circ_0009910 MUC1 were notably increased cancer compared with control tissues, parallel down-regulation miR-145-5p. Clinicopathological analysis indicated that up-regulation tumors related to invasion tumor lymph node (P value = 0.011). Also, downregulation miR-145-5p was significantly correlated nodes 0.04) HER2-negative 0.037). Finally, overexpression age under 45 years 0.002). More importantly, circ_0009910-siRNA decreased proliferation migration ability cells, enhanced miR-145-5p, MUC1. Taken together, has been demonstrated might provide target for treatment.

Language: Английский

Citations

6
Target‐Triggered Formation of Artificial Enzymes on Filamentous Phage for Ultrasensitive Direct Detection of Circulating miRNA Biomarkers in Clinical Samples DOI
Yan Zeng, Yue Hui,

Binrui Cao

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(45)

Published: Sept. 15, 2022

Abstract Integrating artificial enzymes onto nanostructures target‐ and site‐specifically is still a challenge. Here we show that target miRNAs trigger the formation of DNAzyme at tip filamentous phage for detecting miRNA biomarkers. Through an antibody‐modified oligonucleotide, with magnetic nanoparticles on sidewall captures miRNA, inducing extends from through hybridization chain reaction. After separation, resultant complex catalyzes colorimetric reaction signal correlated to concentrations, leading quantification detection limit 5.0 fM, about 10 3 folds lower than phage‐free approach. Our approach can differentiate mutants quantify in human plasma, tumor cells, tissues high sensitivity, suggesting target‐triggered integration phages holds promise searching new catalysts.

Language: Английский

Citations

19
The novel delivery-exosome application for diagnosis and treatment of rheumatoid arthritis DOI
Xinying Yang, Hongmei Xia, Chang Liu

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 242, P. 154332 - 154332

Published: Jan. 21, 2023

Language: Английский

Citations

13
Circulating microRNAs for Early Diagnosis of Ovarian Cancer: A Systematic Review and Meta-Analysis DOI Creative Commons
Nanna Lond Skov Frisk, Anja E. Sørensen, Ole Birger Pedersen

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(5), P. 871 - 871

Published: May 22, 2023

In this study, we conducted a systematic review and meta-analysis to summarize evaluate the global research potential of different circulating miRNAs as an early diagnostic biomarker for OC. A literature search relevant studies was in June 2020 followed up November 2021. The English databases (PubMed, ScienceDirect). primary resulted total 1887 articles, which were screened according prior established inclusion exclusion criteria. We identified 44 studies, 22 eligible quantitative meta-analysis. Statistical analysis performed using Meta-package Rstudio. Standardized mean differences (SMD) relative levels between control subjects OC patients used differential expression. All quality evaluated Newcastle-Ottawa Scale. Based on meta-analysis, nine dysregulated compared controls. Nine upregulated controls (miR-21, -125, -141, -145, -205, -328, -200a, -200b, -200c). Furthermore, miR-26, -93, -106 -200a analyzed, but did not present overall significant difference These observations should be considered when performing future relation OC: sufficient size clinical cohorts, development consensus guidelines miRNA measurements, coverage previously reported miRNAs.

Language: Английский

Citations

13