LncRNA MNX1-AS1 sustains inactivation of Hippo pathway through a positive feedback loop with USP16/IGF2BP3 axis in gallbladder cancer

Cancer Letters, Journal Year: 2022, Volume and Issue: 547, P. 215862 - 215862

Published: Aug. 8, 2022

Language: Английский

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Downregulation of lncRNA MNX1-AS1 promotes the ferroptosis and apoptosis of non-small cell lung cancer

International Journal of Medical Sciences, Journal Year: 2025, Volume and Issue: 22(5), P. 1052 - 1063

Published: Feb. 3, 2025

Non-small cell lung cancer (NSCLC), the main histological type of cancer, poses a serious threat to human health. Increasing evidence has shown that long non-coding RNA (lncRNA) MNX1-AS1 is involved in development and progression cancers, including cancer. Apoptosis ferroptosis, which are two forms regulated death, can be induced by anti-cancer drugs. However, roles apoptosis ferroptosis still unclear. Here, we found knockdown promoted RSL3 NSCLC cells, with decrease viability increases reactive oxygen species (ROS) malondialdehyde (MDA) levels. Meanwhile, acridine orange/ethidium bromide (AO/EB) double staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay Annexin V/PI staining revealed caused paclitaxel cells. In addition, resulted increased expression pro-apoptotic protein BAX as well cleaved caspase-3 PARP1, decreased anti-apoptotic Bcl-2. sequencing quantitative real-time PCR identified ACSL4 was increased, while ABCG2 reduced when knocked down. Rescue showed were MNX1-AS1-mediated apoptosis, respectively. Furthermore, sensitivity cells combination paclitaxel. Taken together, our data suggest might potential therapeutic target for especially and/or apoptosis-inducing

Language: Английский

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The Role of Non-Coding RNAs in MYC-Mediated Metabolic Regulation: Feedback Loops and Interactions

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(2), P. 27 - 27

Published: March 18, 2025

Metabolic reprogramming is a hallmark of cancer, crucial for supporting the rapid energy demands tumor cells. MYC, often deregulated and overexpressed, key driver this shift, promoting Warburg effect by enhancing glycolysis. However, there remains gap in understanding mechanisms factors influencing MYC's metabolic roles. Recently, non-coding RNAs (ncRNAs) have emerged as important modulators MYC functions. This review focuses on ncRNAs that regulate MYC-driven metabolism, particularly effect. The categorizes these into three main groups based their interaction with examines behind interactions. Additionally, we explore how different types may collaborate or influence each other's roles regulation function, aiming to identify biomarkers synthetic lethality targets disrupt cancer. Finaly, highlights clinical implications ncRNAs, providing an up-to-date summary potential cancer prognosis therapy. With recent advances MYC-targeted therapy reaching trials, exciting combining therapies ncRNA-based strategies holds great promise treatment efficacy.

Language: Английский

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Unveiling the functions of five recently characterized lncRNAs in cancer progression

Clinical & Translational Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: July 27, 2024

Language: Английский

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HOTAIR in solid tumors: Emerging mechanisms and clinical strategies

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 154, P. 113594 - 113594

Published: Aug. 31, 2022

Long non-coding RNAs (lncRNAs) play a critical role in several human diseases, particularly solid tumors. Specific lncRNAs are effective biological molecular markers for diagnosing diseases and determining patient prognosis designing molecularly targeted therapies. HOTAIR is reportedly involved physiological emerging processes tumor, such as unlocking phenotypic plasticity, polymorphic microbiome, cellular senescence, has been implicated tumorigenesis progression. This review summarizes the relationship between levels of expression, features, clinical symptoms, regulatory mechanisms action cancers discusses applicability biomarker predicting risk, diagnosis, prognosis, therapy cancer.

Language: Английский

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The Pleiotropic Ubiquitin-Specific Peptidase 16 and Its Many Substrates

Cells, Journal Year: 2023, Volume and Issue: 12(6), P. 886 - 886

Published: March 13, 2023

Ubiquitin-specific peptidase 16 (USP16) is a deubiquitinase that plays role in the regulation of gene expression, cell cycle progression, and various other functions. It was originally identified as major for histone H2A has since been found to deubiquitinate range substrates, including proteins from both cytoplasm nucleus. USP16 phosphorylated when cells enter mitosis dephosphorylated during metaphase/anaphase transition. While much localized cytoplasm, separating enzyme its substrates considered an important regulatory mechanism. Some functions linked include DNA damage repair, immune disease, tumorigenesis, protein synthesis, coronary artery health, male infertility. The strong connection response fact multiple oncogene products are suggests may be potential therapeutic target treatment certain human diseases.

Language: Английский

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Breast Cancer Genomics: Primary and Most Common Metastases

Cancers, Journal Year: 2022, Volume and Issue: 14(13), P. 3046 - 3046

Published: June 21, 2022

Specific genomic alterations have been found in primary breast cancer involving driver mutations that result tumorigenesis. Metastatic cancer, which is uncommon at the time of disease onset, variably impacts patients throughout course their disease. Both molecular profiles and diverse pathways vary development progression metastatic cancer. From most common site (bone), to rare sites such as orbital, gynecologic, or pancreatic metastases, different levels gene expression indicate potential involvement numerous genes spread Knowledge these can, not only help predict future disease, but also lead advancement treatments. This review discusses somatic landscape tumors.

Language: Английский

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Overexpression of LncRNA MNX1-AS1/PPFIA4 Activates AKT/HIF-1α Signal Pathway to Promote Stemness of Colorectal Adenocarcinoma Cells

Journal of Oncology, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 16

Published: Oct. 3, 2022

The purpose of this study was to explore the role lncRNA MNX1-AS1 and its related downstream signaling pathways in colorectal adenocarcinoma (COAD). COAD tissues cells were prepared treated with sh-MNX1-AS1, pcDNA-MNX1-AS1, sh-PPFIA4, LY29004, their controls. CCK8 colony formation assays undertaken for evaluating cell proliferation. Tumor migratory ability detected by transwell assay. Apoptosis detection processed YO-PRO-1/PI Staining. regulated relationship between PPFIA4 confirmed RIP-ChIP Q-PCR applied detect genes tumor stemness, proliferation, migration, apoptosis each group. Finally, a xenograft model constructed verify result vivo. patients high expression have poor prognosis. LncRNA promotes stemness cells. mediates affects stemness. accelerates proliferation while it suppresses apoptosis. MNX1-AS1/PPFIA4 growth model. activates AKT/HIF-1α pathway promote development. LY29004 significantly inhibits tumorigenic PPFIA4. signal cells, which could be new target treatment.

Language: Английский

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High linc01116 expression may contribute to a poor prognosis in various cancers based on systematic reviews and meta-analyses

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: Dec. 23, 2024

Long non-coding RNA 01116 (linc01116) has been shown to be dysregulated in many tumors, and is closely related the prognosis. This meta-analysis aimed examine correlation between linc01116 expression cancer Six electronic databases were searched, eligible studies screened based on inclusion exclusion criteria. Data including hazard ratios (HRs) 95% confidence intervals (CIs), TNM stage, distant metastasis (DM) status, lymph node (LNM) tumor size extracted from included studies. HRs odds (ORs) with their corresponding CIs separately pooled assess relationship Sensitivity analysis Begg's test performed publication or other biases. A total of 12 involving 809 patients this meta-analysis. Analysis showed that high was significantly correlated poor overall survival (OS) (HR = 2.096; CI: 1.555–2.638), progression-free (PFS) (HR, 1.9314; 1.020–3.657), disease-free (DFS) 2.067; 1.0889–3.9238), an advanced stage (OR, 1.803; 1.270–2.562), a histological grade 1.968; 1.288–3.007). However, no significant observed LNM 1.198; 0.831–1.728), DM 1.114; 0.757–1.638), 1.336; 0.989–1.804), depth invasion 1.375; 0.756–2.501), age 0.976; 0.742–1.283), sex 0.810; 0.599–1.094). indicated results OS reliable robust. In addition, none had bias. upregulated most cancers, upregulation associated Therefore, serves as promising therapeutic target prognostic biomarker for cancer.

Language: Английский

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A novel lncRNA BF368575 promotes cell proliferation in hepatocellular carcinoma via PI3K/AKT/mTOR signaling pathway

Research Square (Research Square), Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 8, 2022

Abstract Purpose LncRNA-BF368575 is a novel long non-coding RNA, its biological function in hepatocellular carcinoma (HCC) remains unknown. Methods The expression levels of lncRNA-BF368575 HCC tissues and cell lines were evaluated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). In situ hybridization (ISH) was performed to determine the tissue chip containing 89 paired para-cancerous tissues. Fractionation nuclear cytoplasmic RNA subcellular localization lncRNA-BF368575. Cell viability colony-forming ability determined counting kit-8 (CCK-8) assay colony formation assay. Tumor growth vivo detected using xenograft model. molecular mechanisms investigated Western blotting RNA-Binding Protein Immunoprecipitation (RIP). Results results showed that increased most lines. Moreover, lower level favorable prognostic factor disease-free survival (DFS). mostly localized cytoplasm. vitro experiments downregulation suppressed proliferation activity, yet overexpression leads an opposite result. Furthermore, activates PI3K/AKT/mTOR signaling pathway increasing p-PI3K(Tyr607), p-AKT(Thr308), p-mTOR(Ser2448), p-4E-BP1(Thr37/46), p-S6(Ser235/236). LY294002 could functions RIP shows bind p-4E-BP1(Thr37/46). Conclusion Our study demonstrates enhances both via activating pathway, which implicates our findings may provide potential target for treatments.

Language: Английский

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