Semina Ciências Agrárias,
Journal Year:
2024,
Volume and Issue:
46(1), P. 149 - 168
Published: Dec. 16, 2024
Sirtuins
are
of
central
importance
in
many
cellular
functions
and
promote
cell
survival
under
stress.
However,
little
information
is
available
regarding
the
relationship
between
sirtuins
female
reproductive
biology,
especially
response
to
thermal
This
study
investigated
influence
moderately
high
(40°C)
low
(37°C)
stress
during
vitro
maturation
on
development
competence
bovine
oocytes
embryos.
The
expression
abundance
other
proteins
involved
were
also
studied.
cumulus-oocyte
complexes
(COCs)
Simmental
(Bos
taurus)
cows
underwent
(IVM)
at
different
temperatures
(37°C,
38.5°C
40°C).
Before
maturation,
stained
with
Brilliant
Cresyl
Blue
(BCB)
categorized
as
labeled
(BCB+)
or
unlabeled
(BCB-).
Embryo
production
was
analyzed
IVM
temperatures.
Polar
body
extrusion
evaluated
following
IVM,
mRNA
protein
P53
cumulus
cells
analyzed.
differing
did
not
significantly
alter
polar
cleavage
rates;
however,
significant
differences
blastocyst
observed.
COCs
matured
(control,
37.3%)
had
highest
rate,
contrast
those
37°C
(33.2%)
40°C
(21.5%).
In
all
groups,
rates
higher
for
BCB+
than
BCB-
oocytes.
oocytes,
SIRT1,
SIRT2,
SIRT3,
SIRT5
genes
after
that
before
most
cases,
when
performed
38.5°C.
COCs,
only
SIRT2
remained
unaffected
by
temperature.
summary,
temperature
change
±1.5°C
24
h
oocyte
impaired
embryo
development.
lead
several
biochemical
alterations
granulosa
from
developmental
(BCB+).
Thus,
SIRT1
important
embryonic
may
protect
against
cold
heat
Biomolecules,
Journal Year:
2024,
Volume and Issue:
14(8), P. 970 - 970
Published: Aug. 8, 2024
Osteoporosis
(OP)
is
a
common
metabolic
bone
disease
characterized
by
low
mass,
decreased
mineral
density,
and
degradation
of
tissue
microarchitecture.
However,
our
understanding
the
mechanisms
remodeling
factors
affecting
mass
remains
incomplete.
Sirtuin1
(SIRT1)
nicotinamide
adenine
dinucleotide-dependent
deacetylase
that
regulates
variety
cellular
metabolisms,
including
inflammation,
tumorigenesis,
metabolism.
Recent
studies
have
emphasized
important
role
SIRT1
in
homeostasis.
This
article
reviews
metabolism
OP
also
discusses
therapeutic
strategies
future
research
directions
for
targeting
SIRT1.
Turkish Journal of Biochemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 5, 2025
Abstract
Objectives
Diabetic
cardiomyopathy
(DCM)
is
a
chronic
complication
of
type
2-diabetes
mellitus,
leading
to
heart
failure.
Addressing
DCM
requires
comprehensive
therapeutic
approach.
This
study
examines
the
protective
role
Δ(9)-tetrahydrocannabinol
(THC)
in
by
assessing
its
impact
on
cardiac
function,
inflammation,
oxidative
stress,
and
mitochondrial
health
exploring
involvement
SIRT3/FOXO1
TLR4/NF-κB/NLRP3
signaling
pathways.
Methods
Over
four-week
period,
THC
(1.5
mg/kg,
administered
intraperitoneally)
was
given
type-2
diabetic
Sprague-Dawley
rats.
Cardiac
function
evaluated
using
carotid
catheter
vivo
while
integrity
assessed
through
fluorometric
techniques.
Moreover,
biochemical
biomarkers
protein
expression
key
proteins
were
measured
via
ELISA
immunoblotting.
Results
Compared
untreated
rats,
treatment
showed
pronounced
effects,
improving
reducing
markers
DCM.
It
significantly
lowered
NF-κB,
NLRP3,
IL-1β,
TNF-α
diabetes-induced
stress
heart,
as
evidenced
decreased
malondialdehyde
levels
increased
glutathione
catalase
levels.
also
prevented
membrane
depolarization
reactive
oxygen
species
production
substantially
suppressed
upregulation
TLR4
MyD88
increasing
FOXO1
Inhibition
pathway
3-TYP
reversed
cardioprotective
effects
THC,
negating
NF-κB/NLRP3/IL-1β
without
influencing
TLR4/MyD88.
Conclusions
These
results
underscore
that
offers
significant
protection
against
damage
mitigating
functional
changes
associated
with
DCM,
primarily
modulation
SIRT3/FOXO1/mitochondrial
