Biofilm Formation and Genetic Diversity of Microbial Communities in Anaerobic Batch Reactor with Polylactide (PLA) Addition DOI Open Access
Agnieszka A. Pilarska, Anna Marzec-Grządziel, Emil Paluch

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 10042 - 10042

Published: June 12, 2023

In this paper, an anaerobic digestion (AD) study was conducted on confectionery waste with granular polylactide (PLA) as a cell carrier. Digested sewage sludge (SS) served the inoculum and buffering agent of systems. This article shows results analyses key experimental properties PLA, i.e., morphological characteristics microstructure, chemical composition thermal stability biopolymer. The evaluation quantitative qualitative changes in genetic diversity bacterial communities, performed using state-of-the-art next generation sequencing (NGS) technique, revealed that material significantly enhanced proliferation; however, it does not change microbiome biodiversity, also confirmed via statistical analysis. More intense microbial proliferation (compared to control sample, without PLA digested, CW–control, CW–confectionery waste) may be indicative dual role biopolymer—support medium. Actinobacteria (34.87%) were most abundant cluster while dominant digested samples firmicutes: sample addition carrier (CW–dig.) 68.27%, (CW + PLA) only 26.45%, comparable (CW–control)—19.45%. Interestingly, number proteobacteria decreased CW–dig. (17.47%), but increased CW (39.82%) compared CW–control (32.70%). analysis biofilm formation dynamics BioFlux microfluidic system faster growth surface area for sample. information complemented by observations microorganisms fluorescence microscopy. images showed sections covered consortia.

Language: Английский

Performance and metagenomics analysis of anaerobic digestion of food waste with adding biochar supported nano zero-valent iron under mesophilic and thermophilic condition DOI
Xinzi Wang, Pan Wang,

Xingyao Meng

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 820, P. 153244 - 153244

Published: Jan. 19, 2022

Language: Английский

Citations

54
Microbiome-functionality in anaerobic digesters: A critical review DOI
Xingxing Zhang, Yiwei Wang,

Pengbo Jiao

et al.

Water Research, Journal Year: 2023, Volume and Issue: 249, P. 120891 - 120891

Published: Nov. 18, 2023

Language: Английский

Citations

40
An ecological explanation for carbon source-associated denitrification performance in wastewater treatment plants DOI

Xueshen Wu,

Zhong Yu, Shasha Yuan

et al.

Water Research, Journal Year: 2023, Volume and Issue: 247, P. 120762 - 120762

Published: Oct. 21, 2023

Language: Английский

Citations

33
Mechanistic understanding of acclimation and energy metabolism of acetoclastic methanogens under different substrate to microorganism ratios DOI Creative Commons

Huanhuan Chang,

Bang Du,

Kai He

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 252, P. 118911 - 118911

Published: April 10, 2024

Mechanistic understanding of acetoclastic methanogenesis is pivotal for optimizing anaerobic digestion efficient methane production. In this study, two different operational modes, continuous flow reactor (CFR) and sequencing batch (SBR), accompanied with solids retention times (SRT) 10 days (SBR10d CFR10d) 25 (SBR25d CFR25d) were implemented to elucidate their impacts on microbial communities energy metabolism methanogens in acetate-fed systems. Microbial community analysis revealed that the relative abundance Methanosarcina (16.0% ∼ 46.0%) surpassed Methanothrix (3.7% 22.9%) each reactor. SBRs had potential enrich both Methanosarcina. Compared SBRs, CFRs lower total methanogens. exhibited a superior enrichment reactors 10-day SRT, while preferred be acclimated 25-day SRT. The mode SRT also observed affect distribution acetate-utilizing bacteria, including Pseudomonas, Desulfocurvus, Mesotoga, Thauera. Regarding enzymes involved metabolism, Ech Vho/Vht demonstrated higher abundances at compared whereas Fpo MtrA-H showed than those CFRs. genes encoding ATPase harbored by was Additionally, V/A-type (typically methanogens) CFRs, F-type bacteria) SBRs.

Language: Английский

Citations

9
Using metagenomics to reveal the effects of zero-valent iron with different sizes on the mesophilic and thermophilic anaerobic digestion of sludge DOI
Libin Chen,

Wenqian Li,

Lina Pang

et al.

Biochemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 109688 - 109688

Published: Feb. 1, 2025

Language: Английский

Citations

1
Genome-centric metagenomics revealed functional traits in high-solids anaerobic co-digestion of restaurant food waste, household food waste and rice straw DOI
Binghan Wang, Liangmao Zhang, Jianhong Shi

et al.

Bioresource Technology, Journal Year: 2023, Volume and Issue: 376, P. 128926 - 128926

Published: March 20, 2023

Language: Английский

Citations

22
Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches DOI Creative Commons
Merve Atasoy, William T. Scott, Alberte Regueira

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 73, P. 108363 - 108363

Published: April 22, 2024

In recent years, there has been growing interest in harnessing anaerobic digestion technology for resource recovery from waste streams. This approach evolved beyond its traditional role energy generation to encompass the production of valuable carboxylic acids, especially volatile fatty acids (VFAs) like acetic acid, propionic and butyric acid. VFAs hold great potential various industries biobased applications due their versatile properties. Despite increasing global demand, over 90% are currently produced synthetically petrochemicals. Realizing large-scale VFA streams offers significant eco-friendly opportunities but comes with several key challenges. These include low yields, unstable acid compositions, complex expensive purification methods, post-processing needs. Among these, yield composition stand out as most critical obstacles impacting economic viability competitiveness. paper seeks offer a comprehensive view combining complementary modeling approaches, including kinetic microbial modeling, understand workings communities metabolic pathways production, enhance efficiency, regulate profiles through integration omics bioreactor data.

Language: Английский

Citations

8
A comprehensive review on biogas production from food waste: Exploring cutting-edge technologies and innovations DOI

Vijayakumar Pradeshwaran,

Vijayabharathi Sundaramoorthy,

Ayyadurai Saravanakumar

et al.

Biomass and Bioenergy, Journal Year: 2024, Volume and Issue: 188, P. 107336 - 107336

Published: Aug. 12, 2024

Language: Английский

Citations

8
Current status and future developments of assessing microbiome composition and dynamics in anaerobic digestion systems using metagenomic approaches DOI Creative Commons

Btissam Niya,

Kaoutar Yaakoubi,

Fatima Zahra Beraich

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(6), P. e28221 - e28221

Published: March 1, 2024

The metagenomic approach stands as a powerful technique for examining the composition of microbial communities and their involvement in various anaerobic digestion (AD) systems. Understanding structure, function, dynamics becomes pivotal optimizing biogas process, enhancing its stability improving overall performance. Currently, taxonomic profiling biogas-producing relies mainly on high-throughput 16S rRNA sequencing, offering insights into bacterial archaeal structures AD assemblages correlations with fed substrates process parameters. To delve even deeper, shotgun genome-centric approaches are employed to recover individual genomes from metagenome. This provides nuanced understanding collective functionalities, interspecies interactions, associations abiotic factors. application OMICs systems holds potential revolutionize field, leading more efficient sustainable waste management practices particularly through implementation precision As ongoing research this area progresses, anticipations high further exciting developments future. review serves explore current landscape analyses, focus advancing our comprehension critically evaluating biases recommendations analysis digesters. Its objective is how contemporary can be effectively applied enhance contribute refinement process. marks substantial stride towards achieving comprehensive

Language: Английский

Citations

6
Non-redundant metagenome-assembled genomes of activated sludge reactors at different disturbances and scales DOI Creative Commons
Soheil A. Neshat, Ezequiel Santillan, Hari Seshan

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Aug. 9, 2024

Abstract Metagenome-assembled genomes (MAGs) are microbial reconstructed from metagenomic data and can be assigned to known taxa or lead uncovering novel ones. MAGs provide insights into how microbes interact with the environment. Here, we performed genome-resolved metagenomics on sequencing four studies using batch reactors at microcosm (~25 mL) mesocosm (~4 L) scales inoculated sludge full-scale wastewater treatment plants. These investigated communities in such plants respond two environmental disturbances: presence of toxic 3-chloroaniline changes organic loading rate. We report 839 non-redundant least 50% completeness 10% contamination (MIMAG medium-quality criteria). From these, 399 putative high-quality, while sixty-seven meet MIMAG high-quality criteria. this catalogue represent sixty-eight laboratory-scale used for disturbance experiments, plant which provided source sludge. This dataset aid meta-studies aimed understanding responses disturbances, particularly as ecosystems confront rapid changes.

Language: Английский

Citations

6