Production, Purification, and Characterization of Extracellular Lipases from Hyphopichia wangnamkhiaoensis and Yarrowia deformans DOI Creative Commons

Misael Romo-Silva,

Emanuel Osmar Flores-Camargo,

Griselda Ma. Chávez-Camarillo

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 595 - 595

Published: Nov. 21, 2024

The efficient production of microbial lipases from organic wastes has garnered great interest because the diverse and potential biotechnological applications these enzymes. However, extracellular novel yeast strains Hyphopichia wangnamkhiaoensis Yarrowia deformans remain uncharacterized. Thus, this study aimed to investigate characteristics both yeasts. Lipases H. Y. were purified biochemically characterized, their was measured in batch cultures with olive oil (reference), waste cooking oil, glycerol as substrates. had molecular weights approximately 33 45 kDa, respectively. Their activities on p-nitrophenyl palmitate optimal at pH 8.0 40 °C. Moreover, inhibited by ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, 4-(2-aminoethyl)benzenesulfonyl reactivated Ca2+ Mg2+, indicating that are metalloenzymes serine-type more tolerant hydrophilic solvents than hydrophobic solvents, they followed Michaelis–Menten kinetics. Among various substrates used, yielded highest lipase productivity. These results indicate suitable candidates for production.

Language: Английский

Sustainable lipid production by oleaginous yeasts: Current outlook and challenges DOI
Kukkala Kiran Kumar, Farha Deeba, Ajay Kumar Pandey

et al.

Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132205 - 132205

Published: Feb. 1, 2025

Language: Английский

Citations

1
Unlocking potential: exploring the methane production potential in anaerobic co-digestion of cassava wastewater and glycerol DOI
Kauanna Uyara Devens,

Alexandre Rodrigues Ribeiro,

Franciele Pereira Camargo

et al.

Journal of environmental chemical engineering, Journal Year: 2024, Volume and Issue: 12(3), P. 113043 - 113043

Published: May 12, 2024

Language: Английский

Citations

4
Rhodotorula sp. as a promising host for microbial cell factories DOI

Baisong Tong,

Yi Yu, Shuobo Shi

et al.

Metabolic Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Optimizing lipid production in oleaginous yeasts for sustainable bioenergy: A review of process parameters, cultivation strategies, and machine learning integration DOI

Wannapawn Watsuntorn,

Nuttha Chuengcharoenphanich,

Piroonporn Srimongkol

et al.

Biomass and Bioenergy, Journal Year: 2025, Volume and Issue: 197, P. 107810 - 107810

Published: March 29, 2025

Language: Английский

Citations

0
Rhodotorula mucilaginosa: kinetics and production of lipids and fatty acids in agroindustrial byproducts DOI
Tábita Veiga Dias Rodrigues, Maristela Barnes Rodrigues Cerqueira, Camila Ramão Contessa

et al.

Brazilian Journal of Chemical Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Language: Английский

Citations

0
Potential of oleaginous microbes for lipid accumulation and renewable energy generation DOI

Yulu Yang,

Mohammed Jalalah, Saeed A. Alsareii

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(11)

Published: Oct. 3, 2024

Language: Английский

Citations

3
Kinetics of Riboflavin Production by Hyphopichia wangnamkhiaoensis under Varying Nutritional Conditions DOI Open Access
Raziel Arturo Jiménez-Nava, Griselda Ma. Chávez-Camarillo, Eliseo Cristiani‐Urbina

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9430 - 9430

Published: Aug. 30, 2024

Riboflavin, an essential vitamin for humans, is extensively used in various industries, with its global demand being met through fermentative processes. Hyphopichia wangnamkhiaoensis a novel dimorphic yeast species capable of producing riboflavin. However, the nutritional factors affecting riboflavin production this remain unknown. Therefore, we conducted kinetic study on effects factors—carbon and energy sources, nitrogen vitamins, amino acids—on batch by H. wangnamkhiaoensis. Batch experiments were performed bubble column bioreactor to evaluate cell growth, substrate consumption, production. The highest was obtained when growth medium supplemented glucose, ammonium sulfate, biotin, glycine. Using these chemical components, along mineral salts from Castañeda-Agullo’s culture medium, formulated novel, low-cost, effective (the RGE medium) This resulted levels volumetric productivity, reaching 16.68 mg/L 0.713 mg/L·h, respectively, within 21 h incubation. These findings suggest that wangnamkhiaoensis, shorter incubation time, could improve efficiency cost-effectiveness industrial production, paving way more sustainable methods.

Language: Английский

Citations

2
Nitrogen limitation-induced adaptive response and lipogenesis in the Antarctic yeast Rhodotorula mucilaginosa M94C9 DOI Creative Commons

Miguel Rosas-Paz,

Alberto Zamora-Bello,

Nayeli Torres‐Ramírez

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 5, 2024

The extremotolerant red yeast

Language: Английский

Citations

0
Production, Purification, and Characterization of Extracellular Lipases from Hyphopichia wangnamkhiaoensis and Yarrowia deformans DOI Creative Commons

Misael Romo-Silva,

Emanuel Osmar Flores-Camargo,

Griselda Ma. Chávez-Camarillo

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 595 - 595

Published: Nov. 21, 2024

The efficient production of microbial lipases from organic wastes has garnered great interest because the diverse and potential biotechnological applications these enzymes. However, extracellular novel yeast strains Hyphopichia wangnamkhiaoensis Yarrowia deformans remain uncharacterized. Thus, this study aimed to investigate characteristics both yeasts. Lipases H. Y. were purified biochemically characterized, their was measured in batch cultures with olive oil (reference), waste cooking oil, glycerol as substrates. had molecular weights approximately 33 45 kDa, respectively. Their activities on p-nitrophenyl palmitate optimal at pH 8.0 40 °C. Moreover, inhibited by ethylenediaminetetraacetic acid, phenylmethylsulfonyl fluoride, 4-(2-aminoethyl)benzenesulfonyl reactivated Ca2+ Mg2+, indicating that are metalloenzymes serine-type more tolerant hydrophilic solvents than hydrophobic solvents, they followed Michaelis–Menten kinetics. Among various substrates used, yielded highest lipase productivity. These results indicate suitable candidates for production.

Language: Английский

Citations

0