Industrial & Engineering Chemistry Research,
Journal Year:
2024,
Volume and Issue:
63(33), P. 14469 - 14479
Published: Aug. 7, 2024
Yarrowia
lipolytica
is
an
unconventional
oleaginous
yeast
with
special
physiological
characteristics.
Due
to
its
high
flux
of
acetyl-CoA,
extensive
substrate
spectra,
clear
gene
sequences,
and
mature
genetic
modification
tools,
it
has
received
widespread
attention
as
a
chassis
strain
for
the
production
various
compounds.
In
this
Perspective,
we
summarize
biosynthetic
pathway
terpenoids.
The
advantages
application
prospects
using
Y.
produce
natural
products
are
introduced.
Strategies
further
improve
metabolite
efficiency
also
discussed,
which
will
provide
references
guidance
expanding
lipolytica.
At
present,
used
variety
biochemicals.
With
development
synthetic
biology,
transformation
tools
can
be
developed
in
future
expression
capability.
It
not
only
simplify
operation
process,
but
promote
yield.
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
72(17), P. 9984 - 9993
Published: April 18, 2024
Squalene
is
a
high-value
antioxidant
with
many
commercial
applications.
The
use
of
microbial
cell
factories
to
produce
squalene
as
an
alternative
plant
and
animal
extracts
could
meet
increasing
market
demand.
Yarrowia
lipolytica
excellent
host
for
production
due
its
high
levels
acetyl-CoA
hydrophobic
environment.
However,
the
need
precise
complicated
gene
editing
has
hindered
industrialization
this
strain.
Herein,
rapid
construction
strain
was
achieved
by
enhancing
homologous
recombination
efficiency
in
Y.
lipolytica.
First,
remodeling
resulted
10-fold
increase
rate.
Next,
whole
mevalonate
pathway
integrated
into
chromosome
enhance
production.
Then,
higher
level
accumulation
acetyl
coenzyme
A
regulating
downstream
steroid
synthesis
pathway.
Finally,
reached
35
g/L
after
optimizing
fermentation
conditions
performing
fed-batch
culture
5
L
jar
fermenter.
This
highest
ever
reported
date
de
novo
biosynthesis
without
adding
any
inhibitors,
paving
new
path
toward
industrial
products.
Metabolic Engineering Communications,
Journal Year:
2024,
Volume and Issue:
18, P. e00240 - e00240
Published: June 1, 2024
Squalene
is
a
highly
sought-after
triterpene
compound
in
growing
demand,
and
its
production
offers
promising
avenue
for
circular
economy
practices.
In
this
study,
we
applied
metabolic
engineering
principles
to
enhance
squalene
the
nonconventional
yeast
Journal of Agricultural and Food Chemistry,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 3, 2024
Squalene
is
a
linear
polyunsaturated
triterpene
which
has
multiple
physiological
functions
including
anticancer,
antioxidant,
and
skin-care.
It
been
widely
used
in
the
food,
medicine,
cosmetics
sectors
also
serves
as
precursor
of
triterpenes
steroids.
Recently,
production
squalene
by
microbial
cell
hosts
drawn
much
attention
due
to
its
sustainability,
environmental
friendliness,
great
efficiency.
In
this
review,
we
first
introduce
recent
developments
employing
factories,
especially
yeasts.
Next,
underscore
primary
metabolic
strategies,
biosynthetic
pathway
engineering,
manipulation,
cofactor
organelle
engineering.
addition
traditional
engineering
discuss
some
prospective
regulation
approaches,
lipid
synthesis,
identifying
manipulating
related
transcription
factors,
dynamic
pathway,
secretion
membrane-impermeable
terpenoids.
These
approaches
provide
insights
for
further
ACS Synthetic Biology,
Journal Year:
2024,
Volume and Issue:
13(8), P. 2545 - 2554
Published: June 11, 2024
is
a
potential
workhorse
for
production
of
various
value-added
chemicals
including
terpenoids,
oleo-chemicals,
and
enzymes
from
low-cost
feedstocks.
However,
the
limited
genetic
toolbox
hindering
its
metabolic
engineering.
In
present
study,
four
type
I
one
novel
II
peroxisomal
targeting
signal
(PTS1/PTS2)
were
characterized
employed
limonene
first
time
in
