Catalytic Hairpin Assembly-Driven Ratiometric Dual-Signal Electrochemical Biosensor for Ultrasensitive Detection of MicroRNA Based on the Ratios of Fe-MOFs and MB-GA-UiO-66-NH₂

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(15), P. 5846 - 5855

Published: April 5, 2022

In this work, a novel ratio electrochemical biosensing platform based on catalytic hairpin assembly target recovery to trigger dual-signal output was developed for ultrasensitive detection of microRNA (miRNA). To achieve the ratiometric strategy, methylene blue (MB), an indicator, ingeniously loaded into pores graphene aerogel (GA) and metal-organic framework (MOF) composites with high porosity large specific surface area, another indicator Fe-MOFs distinct separation redox potential selected as signal probe. Concretely, presence miRNA, CHA process initiated probe introduced electrode surface, producing abundant double-stranded H1-H2@Fe-MOFs-NH2. Then, measurement analysis prepared biosensor by differential pulse voltammetry (DPV) showed that introduction miRNA led increase in oxidation peak (+0.8 V) decrease MB (-0.23 V). Therefore, current IFe-MOFs/IMB could be employed accurately reflect actual concentration miRNA. Under optimal conditions, limit proposed down 50 aM. It worth noting exhibited excellent performance complex serum environment tumor cell lysates, showing great clinical diagnosis.

Language: Английский

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Flexible Self-Powered Sensing System Based on Novel DNA Circuit Strategy and Graphdiyne for Thalassemia Gene by Rapid Naked-Eye Tracking and Open-Circuit Voltage

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(44), P. 16374 - 16382

Published: Oct. 23, 2023

Based on the controllable instantaneous self-assembly ability of long-chain branched DNA nanostructures and synergistic effect between nucleic acid amplification without enzymes, a highly sensitive specific self-powered biosensing platform is developed. Two-dimensional graphdiyne prepared, modified flexible carbon cloth, then functionalized with gold nanoparticles. When mi-tubes are applied it, target thalassemia gene CD122 triggers dual-catalytic hairpin assembly reaction. The generated nanoscale precisely captured by mi-tube, exposing binding sites activating hybridization chain reaction to form DNA. Double-stranded DNA, along dendritic carrying large number guanine bases, captures signal molecule methylene blue (MB), generating significant electrochemical signal. redox MB also causes proportional change in system's color, achieving colorimetric detection functionality. An efficient dual-mode sensing platform, therefore, established for detecting CD122. linear response range concentration open-circuit voltage RGB Blue value 0.0001–10,000 pM. limit under mode 36.3 aM (S/N = 3), mode, it as low 12.1 3). new method exhibits high sensitivity, excellent selectivity, accuracy, providing universal strategy designing novel platforms that can be extended other biomolecules.

Language: Английский

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Iridium Complex with Specific Intercalation in the G-Quadruplex: A Phosphorescence and Electrochemiluminescence Dual-Mode Homogeneous Biosensor for Enzyme-Free and Label-Free Detection of MicroRNA

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(4), P. 1529 - 1535

Published: March 24, 2023

Design and exploitation of high-property multifunctional emitters for achieving immobilization-free, label-free, enzyme-free, dual-mode determination microRNA (miRNA) with high sensitivity selectivity are particularly urgent but remain a huge challenge. Herein, the first time, we reported an Ir3+-based N-heterocyclic complex, namely, IrPyPt, ascertained its phosphorescence electrochemiluminescence (ECL) dual-emission behavior in homogeneous solution, discovered turn-up signal-off ECL response to G-quadruplex DNA over other DNAs. Inspired by this, IrPyPt was pioneered as emitter combine nucleic acid probes (ON1 ON2) developing biosensor, which applied highly sensitive, credible analysis miRNA-21 used target analyte. triggered hybridization chain reaction ON1 ON2 generate abundant G-quadruplexes, caught yield IrPyPt@G-quadruplex, intramolecular rotary motion inhibited unable diffuse electrode, contributing boosted emission sharply declined emission. With this design, achieved limits detection down 1.40 0.18 pM, respectively. Furthermore, biosensor triumphantly determine concentration cancer cell lysates thus provided great potential alternative miRNA-related diseases' early accurate diagnosis.

Language: Английский

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Nanozyme‐Enhanced Electrochemical Biosensors: Mechanisms and Applications

Small, Journal Year: 2023, Volume and Issue: 20(14)

Published: Nov. 20, 2023

Abstract Nanozymes, as innovative materials, have demonstrated remarkable potential in the field of electrochemical biosensors. This article provides an overview mechanisms and extensive practical applications nanozymes First, definition characteristics are introduced, emphasizing their significant role constructing efficient sensors. Subsequently, several common categories nanozyme materials delved into, including metal‐based, carbon‐based, metal‐organic framework, layered double hydroxide nanostructures, discussing Regarding mechanisms, two key roles particularly focused biosensors: selective enhancement signal amplification, which crucially support sensor performance. In terms applications, widespread use nanozyme‐based biosensors showcased various domains. From detecting biomolecules, pollutants, nucleic acids, proteins, to cells, providing robust means for high‐sensitivity detection. Furthermore, insights into future development is provided, encompassing improvements optimizations design integration, expansion application fields through interdisciplinary collaboration. conclusion, this systematically presents biosensors, offering valuable references prospects research field.

Language: Английский

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Cascade amplification strategy based on ultra-thin graphdiyne and CRISPR/Cas for real-time detection of tumor biomarker

Chemical Engineering Journal, Journal Year: 2023, Volume and Issue: 466, P. 143230 - 143230

Published: April 29, 2023

Language: Английский

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Flexible SERS sensor using AuNTs-assembled PDMS film coupled chemometric algorithms for rapid detection of chloramphenicol in food

Food Chemistry, Journal Year: 2023, Volume and Issue: 418, P. 135998 - 135998

Published: March 28, 2023

Language: Английский

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Laser-induced graphene (LIG)-based electrochemical microfluidic chip for simultaneous analysis of multiplex microRNAs

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 486, P. 150233 - 150233

Published: March 7, 2024

Language: Английский

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DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(4), P. 1733 - 1741

Published: Jan. 16, 2024

Exosomal miRNAs are considered promising biomarkers for cancer diagnosis, but their accuracy is severely compromised by the low content of and large amount exosomal released from normal cells. Here, we presented a dual-specific miRNA's logical recognition triggered an entropy-driven catalysis (EDC)-enhanced system in exosomes accurate detection liver cancer-cell-derived miR-21 miR-122. Taking advantage analytical performance logic device, excellent membrane penetration gold nanoparticles, outstanding amplification ability EDC reaction, this method exhibits high sensitivity selectivity tumor-derived situ. Moreover, due to its performance, device can effectively distinguish patients healthy donors determining miRNAs. Overall, strategy has great potential analyzing various types provides viable tool improve diagnosis.

Language: Английский

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Intramolecular DNA Wheel Construction for Highly Sensitive Electrochemical Detection of miRNA

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Accurate and reliable quantification of disease-related biomolecules is essential for clinical diagnosis. In this study, a novel electrochemical approach developed based on target triggered DNA nanostructural switch from hairpin dimer to double-stranded wheel. During the process, species get closer electrode interface, multiple intramolecular strand displacements are beneficial low abundant analysis. addition, use nicking endonuclease mitigates background interference. This strategy enables highly sensitive selective miRNA. A linear relationship established between peak current intensity logarithm miRNA concentration within range 0.1 20 fM. method also demonstrates high accuracy in analysis samples, holding great potential nanotechnology diagnostic applications.

Language: Английский

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A dual-amplification mode and Cu-based metal-organic frameworks mediated electrochemical biosensor for sensitive detection of microRNA

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 202, P. 113992 - 113992

Published: Jan. 10, 2022

Language: Английский

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