Beyond traditional biosensors: Recent advances in gold nanoparticles modified electrodes for biosensing applications

Talanta, Journal Year: 2023, Volume and Issue: 268, P. 125280 - 125280

Published: Oct. 12, 2023

Language: Английский

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Application of microfluidic technology based on surface-enhanced Raman scattering in cancer biomarker detection: A review

Journal of Pharmaceutical Analysis, Journal Year: 2023, Volume and Issue: 13(12), P. 1429 - 1451

Published: Aug. 24, 2023

With the continuous discovery and research of predictive cancer-related biomarkers, liquid biopsy shows great potential in cancer diagnosis. Surface-enhanced Raman scattering (SERS) microfluidic technology have received much attention among various biomarker detection methods. The former has ultrahigh sensitivity can provide a unique fingerprint. In contrast, latter characteristics miniaturization integration, which realize accurate control samples high-throughput through design. Both for point-of-care testing (POCT), their combination (lab-on-a-chip SERS (LoC-SERS)) good compatibility. this paper, basic situation circulating proteins, tumor cells, exosomes, DNA (ctDNA), microRNA (miRNA) diagnosis cancers is reviewed, these biomarkers by LoC-SERS platform recent years described detail. At same time, challenges future development are discussed at end review. Summarizing current expected to reference scholars engaged related work interested field.

Language: Английский

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Recent Developments in Inertial and Centrifugal Microfluidic Systems along with the Involved Forces for Cancer Cell Separation: A Review

Sensors, Journal Year: 2023, Volume and Issue: 23(11), P. 5300 - 5300

Published: June 2, 2023

The treatment of cancers is a significant challenge in the healthcare context today. Spreading circulating tumor cells (CTCs) throughout body will eventually lead to cancer metastasis and produce new tumors near healthy tissues. Therefore, separating these invading extracting cues from them extremely important for determining rate progression inside development individualized treatments, especially at beginning process. continuous fast separation CTCs has recently been achieved using numerous techniques, some which involve multiple high-level operational protocols. Although simple blood test can detect presence circulation system, detection still restricted due scarcity heterogeneity CTCs. more reliable effective techniques thus highly desired. technology microfluidic devices promising among many other bio-chemical bio-physical technologies. This paper reviews recent developments two types devices, are based on size and/or density cells, cells. goal this review identify knowledge or gaps suggest future works.

Language: Английский

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3D-Nanostructured microfluidic device arranged in a herringbone pattern for the highly effective capture of HER2-Positive cancer-derived exosomes in urine

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 482, P. 148851 - 148851

Published: Jan. 18, 2024

Exosomes in body fluids are important liquid biopsy as they reflect the information of their originating cells. Thus, exosome analysis can provide valuable insights into diseases such cancer. However, low concentration exosomes is accompanied by background noise, making challenging. Here, a microfluidic chip which three-dimensional nanostructures were arranged herringbone pattern (nanochip) that could efficiently capture specific was developed. Nanostructures prepared stacking silica nanoparticles to enhance contact and interaction between structures, then improve mass transfer through micromixing. To analyze derived from human epidermal growth factor receptor 2 (HER2, an marker for cancer progression patient survival)-positive cancer, anti-HER2 antibody introduced nanochip approximately 97.7% efficiency confirmed. The performed better than chips with solid structure or without (solid flat chips). feasibility capturing multiple demonstrated using both vitro vivo samples employing dual configuration nanochips different antibodies interconnected series. This effectively HER2-positive has potential isolations. Additionally, this detect various disease-related because be applied; will useful exosome-based disease diagnosis monitoring biopsies.

Language: Английский

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Development of an MIP based electrochemical sensor for TGF-β1 detection and its application in liquid biopsy

The Analyst, Journal Year: 2023, Volume and Issue: 148(18), P. 4447 - 4455

Published: Jan. 1, 2023

Development and characterization of TGF-β1 MIP electrochemical sensor as a tool for POC diagnostics.

Language: Английский

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Surface‐Enhanced Raman Spectroscopy‐Based Optical Biosensor for Liquid Biopsy: Toward Precision Medicine

Laser & Photonics Review, Journal Year: 2024, Volume and Issue: 18(5)

Published: Jan. 28, 2024

Abstract Liquid biopsy is regarded as a promising strategy for assisting precision medicine because of its convenience, noninvasiveness, and ability to overcome tumor heterogeneity achieve early detection. Recently, impressive advancements in plasmonic biosensors, artificial intelligence, portable Raman equipment have yielded unprecedented progress surface‐enhanced spectroscopy (SERS)–based point‐of‐care testing (POCT) systems liquid biopsy. The development these presents paradigm shift on‐site applications by leveraging the unique benefits efficiency, fast analysis, portability, affordability, user‐friendliness. Herein, advances are introduced over last 3 years field SERS‐based POCT labeled label‐free biomarker analysis body fluids, including circulating proteins cells, exosomes, micro‐RNA, DNA. Additionally, powerful machine learning algorithms (including deep algorithms) integrated with SERS effectively extract potential data features generate precise diagnostic models. review highlights use handheld devices significantly promoting application clinical scenarios. Finally, outlines challenges future perspectives this technology.

Language: Английский

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Cutaneous Oncology: Strategies for Melanoma Prevention, Diagnosis, and Therapy

Cancer Control, Journal Year: 2024, Volume and Issue: 31

Published: Jan. 1, 2024

Skin cancer comprises one-third of all diagnosed cases and remains a major health concern. Genetic environmental parameters serve as the two main risk factors associated with development skin cancer, ultraviolet radiation being most common factor. Studies have also found fair complexion, arsenic toxicity, indoor tanning, family history among prevailing causes cancer. Prevention early diagnosis play crucial role in reducing frequency ensuring effective management Recent studies focused on exploring minimally invasive or non-invasive diagnostic technologies along artificial intelligence to facilitate rapid accurate diagnosis. The treatment ranges from traditional surgical excision various advanced methods such phototherapy, radiotherapy, immunotherapy, targeted therapy, combination therapy. introduction new checkpoint inhibitors personalized immunotherapy enhancing efficacy. Advancements multi-omics, nanotechnology, further deepened understanding mechanisms underlying tumoral growth their interaction therapeutic effects, which has paved way for precision oncology. This review aims highlight recent advancements provide an overview existing emerging diagnostic, prognostic, modalities, while highlighting areas that require research bridge knowledge gaps.

Language: Английский

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Miniaturized Real-Time PCR systems for SARS-CoV-2 detection at the Point-of-Care

Clinica Chimica Acta, Journal Year: 2022, Volume and Issue: 536, P. 104 - 111

Published: Sept. 17, 2022

Language: Английский

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Urinary Cell-Free DNA in Liquid Biopsy and Cancer Management

Clinical Chemistry, Journal Year: 2022, Volume and Issue: 68(12), P. 1493 - 1501

Published: Aug. 24, 2022

Abstract Background The current methodology used to detect, diagnose, and monitor many types of cancers requires invasive tissue biopsy testing. Recently, liquid using blood, plasma, urine, saliva, various other bodily fluids has shown utility solve issues associated with biopsy. Blood/plasma received most the attention within field, however, obtaining blood samples from patients is still somewhat trained professionals. Using urine detect cell-free DNA cancer biomarkers offers a truly non-invasive sampling method that can be easily reproducibly conducted by patients. Content Novel technologies approaches have made detection small quantities tumor varying lengths possible. Recent studies circulating mutations sensitivity comparable blood/plasma for types. Thus, may replace or provide supplementary information tissue/blood biopsies. Further investigation larger patient cohorts standardization pre-analytical factors necessary determine detection, diagnosis, monitoring in clinical setting. Summary In this mini-review we discuss biological aspects numerous urological cancers, recent non-urological including lung, breast, colorectal, cancers.

Language: Английский

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A Novel Hybrid Platform for Live/Dead Bacteria Accurate Sorting by On-Chip DEP Device

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7077 - 7077

Published: April 11, 2023

According to the World Health Organization (WHO) forecasts, Antimicrobial Resistance (AMR) will be leading cause of death worldwide in next decades. To prevent this phenomenon, rapid Susceptibility Testing (AST) techniques are required drive selection most suitable antibiotic and its dosage. In context, we propose an on-chip platform, based on a micromixer microfluidic channel, combined with pattern engineered electrodes exploit di-electrophoresis (DEP) effect. The role is ensure proper interaction bacteria over long time (≈1 h), DEP-based channel enables efficient sorting live from dead bacteria. A efficiency more than 98%, low power consumption (Vpp = 1 V) response 5 s, within chip footprint ≈86 mm2, has been calculated, which makes proposed system very attractive innovative for monitoring antimicrobial susceptibility at single-bacterium level next-generation medicine.

Language: Английский

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