Critical Reviews in Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown, P. 1 - 16
Published: March 24, 2025
Fluorescent
sensor
is
an
important
tool
to
reliaze
qualitative
or
quantitative
detection
of
target
analyte
based
on
the
fluorescence
principle.
Clustered
regularly
interspaced
short
palindromic
repeats/CRISPR-associated
protein
(CRISPR/Cas)
has
been
utilized
develop
as
a
precise,
efficient,
and
highly
sensitive
molecular
diagnostic
due
its
efficient
targeting
gene
editing
ability.
At
present,
CRISPR/Cas
system-based
fluorescent
sensors
have
shown
excellent
performance
in
field
analysis
detection,
received
widespread
attention.
Therefore,
this
paper
reviews
mechanism
system,
characteristics
different
Cas
proteins,
principle
sensor,
with
focus
summarizing
application
for
detection.
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(24), P. 8500 - 8530
Published: Jan. 1, 2023
This
review
explores
various
point-of-care
optical
diagnostic
systems
combined
with
microdevices
developed
during
the
recent
COVID-19
pandemic
for
clinical
diagnostics.
Lab on a Chip,
Journal Year:
2024,
Volume and Issue:
24(5), P. 1441 - 1493
Published: Jan. 1, 2024
This
review
explores
emerging
microfluidic-based
technologies
incorporating
innovative
assay
strategies
for
infectious
disease
diagnostics.
Our
classification
scheme
is
based
on
the
human
body
systems
or
pathogen
transmission
modes.
Micromachines,
Journal Year:
2022,
Volume and Issue:
13(8), P. 1238 - 1238
Published: Aug. 1, 2022
A
microfluidic
chip
is
a
tiny
reactor
that
can
confine
and
flow
specific
amount
of
fluid
into
channels
tens
to
thousands
microns
as
needed
precisely
control
flow,
pressure,
temperature,
etc.
Point-of-care
testing
(POCT)
requires
small
equipment,
has
short
cycles,
controls
the
process,
allowing
single
or
multiple
laboratory
facilities
simultaneously
analyze
biological
samples
diagnose
infectious
diseases.
In
general,
rapid
detection
stage
assessment
viral
epidemics
are
essential
overcome
pandemic
situations
promptly.
Therefore,
combining
devices
with
POCT
improves
efficiency
convenience
for
disease
SARS-CoV-2.
At
same
time,
chips
increases
user
accessibility,
accuracy
sensitivity,
shortens
etc.,
which
beneficial
in
detecting
This
review
shares
recent
advances
POCT-based
COVID-19
how
it
better
suited
help
response
ongoing
pandemic.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 12, 2024
The
CRISPR/Cas
system
is
a
powerful
genome
editing
tool
and
possesses
widespread
applications
in
molecular
diagnostics,
therapeutics
genetic
engineering.
But
easy
folding
of
the
target
sequences
causes
remarkable
deterioration
recognition
shear
efficiency
case
single
Cas-CRISPR
RNA
(crRNA)
duplex.
Here,
we
develop
cooperative
shearing
(CRISPR-CS)
system.
Compared
with
traditional
system,
two
CRISPR/Cas-crRNA
duplexes
simultaneously
recognize
different
sites
sequence,
increasing
possibility
efficiency.
Cooperative
cuts
more
methylene
blue-ssDNA
reporters
on
electrode,
enabling
unamplified
nucleic
acid
electrochemical
assay
less
than
5
minutes
detection
limit
9.5×10
