Clinical application and detection techniques of liquid biopsy in gastric cancer

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Jan. 11, 2023

Abstract Gastric cancer (GC) is one of the most common tumors worldwide and leading cause tumor-related mortality. Endoscopy serological tumor marker testing are currently main methods GC screening, treatment relies on surgical resection or chemotherapy. However, traditional examination more harmful to patients less sensitive accurate. A minimally invasive method respond early prognosis monitoring, efficacy, drug resistance situations urgently needed. As a result, liquid biopsy techniques have received much attention in clinical application GC. The non-invasive technique requires fewer samples, reproducible, can guide individualized patient by monitoring patients' molecular-level changes real-time. In this review, we introduced applications circulating cells, free DNA, non-coding RNAs, exosomes, proteins, which primary markers technology We also discuss current limitations future trends as applied technology.

Language: Английский

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CRISPR/Cas12a-derived ratiometric fluorescence sensor for high-sensitive Pb2+ detection based on CDs@ZIF-8 and DNAzyme

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 251, P. 116089 - 116089

Published: Feb. 3, 2024

Language: Английский

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Optical Nanobiosensor Based on Surface-Enhanced Raman Spectroscopy and Catalytic Hairpin Assembly for Early-Stage Lung Cancer Detection via Blood Circular RNA

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 2020 - 2030

Published: April 11, 2024

Lung cancer has become the leading cause of cancer-related deaths globally. However, early detection lung remains challenging, resulting in poor outcomes for patients. Herein, we developed an optical biosensor integrating surface-enhanced Raman spectroscopy (SERS) with a catalyzed hairpin assembly (CHA) to detect circular RNA (circRNA) associated tumor formation and progression (circSATB2). The signals reporter were considerably enhanced by generating abundant SERS "hot spots" core–shell nanoprobe 2D substrate calibration capabilities. This approach enabled sensitive (limit detection: 0.766 fM) reliable quantitative target circRNA. Further, used circRNA human serum samples, revealing that patients had higher concentrations than healthy subjects. Moreover, characterized unique concentration profiles stages (IA IB) subtypes (IA1, IA2, IA3) cancer. These results demonstrate potential proposed sensing nanoplatform as liquid biopsy prognostic tool screening

Language: Английский

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Nucleic acid-templated silver nanoclusters: A review of structures, properties, and biosensing applications

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 491, P. 215247 - 215247

Published: May 27, 2023

Language: Английский

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Application of microfluidic technology based on surface-enhanced Raman scattering in cancer biomarker detection: A review

Journal of Pharmaceutical Analysis, Journal Year: 2023, Volume and Issue: 13(12), P. 1429 - 1451

Published: Aug. 24, 2023

With the continuous discovery and research of predictive cancer-related biomarkers, liquid biopsy shows great potential in cancer diagnosis. Surface-enhanced Raman scattering (SERS) microfluidic technology have received much attention among various biomarker detection methods. The former has ultrahigh sensitivity can provide a unique fingerprint. In contrast, latter characteristics miniaturization integration, which realize accurate control samples high-throughput through design. Both for point-of-care testing (POCT), their combination (lab-on-a-chip SERS (LoC-SERS)) good compatibility. this paper, basic situation circulating proteins, tumor cells, exosomes, DNA (ctDNA), microRNA (miRNA) diagnosis cancers is reviewed, these biomarkers by LoC-SERS platform recent years described detail. At same time, challenges future development are discussed at end review. Summarizing current expected to reference scholars engaged related work interested field.

Language: Английский

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A programmable quantum dot nanosensor guided by three-way junction skeleton-mediated cascade signal amplification for sensitive detection of circRNAs in breast cancer

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 484, P. 149788 - 149788

Published: Feb. 17, 2024

Language: Английский

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A dual-mode nanoplatform based on Cu2O@Au-Pt nanoenzyme and CHA-HCR DNA framework circuit for sensitive detection of CD122 and CD17

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142740 - 142740

Published: April 1, 2025

Language: Английский

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Docking-aided rational tailoring of a fluorescence- and affinity-enhancing aptamer for a label-free ratiometric malachite green point-of-care aptasensor

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 447, P. 130798 - 130798

Published: Jan. 14, 2023

Language: Английский

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Construction of Genetically Encoded Light-Up RNA Aptamers for Label-free and Ultrasensitive Detection of CircRNAs in Cancer Cells and Tissues

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(22), P. 8728 - 8734

Published: May 23, 2023

Circular RNAs (circRNAs) as endogenous non-coding are characterized by covalently closed circular structures, and they widely exist in mammalian cells. The aberrant expression of circRNAs may result various diseases. Herein, we demonstrate the construction genetically encoded light-up RNA aptamers for ultrasensitive label-free detection circRNA mitochondrial tRNA translation optimization 1 (circMTO1) cancer cells tissues. generated proximity ligation-activated recombinase polymerase amplification (RPA)-assisted transcription amplification. When circMTO1 is present, it initiates ligation reaction, activating RPA to produce numerous long double-stranded DNAs containing T7 promoters. Subsequently, products identified polymerase, initiating reaction generate abundant Spinach aptamers. can bind with DFHBI (3,5-difluoro-4-hydroxybenzylidene imidazolidinone) dye a distinct fluorescence signal near-zero background. This biosensor exhibits excellent selectivity high sensitivity limit 2.54 aM. It accurately monitor cellular at single-cell level discriminate between breast patient tissues healthy Notably, this be employed measure other nucleic acids altering corresponding target recognition sequences, providing valuable platform diagnosis biomedical study.

Language: Английский

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Fluorescence Biosensing Based on Bifurcated DNA Scaffold-Aggregated Ag Nanocluster via Responsive Conformation Switch of Quasi-Molecular Beacon

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(8), P. 3480 - 3488

Published: Feb. 14, 2024

To address the limitations of typical hairpin-structural molecular beacons, exploring ability a quasi-molecular beacon (qMB) to create label-free fluorescence biosensors is intriguing and remains challenge. Herein, we propose first example modular qMB with feature stimulation-responsive conformation switch develop an aggregated Ag nanocluster (aAgNC) in bifurcated DNA scaffold for fluorescently sensing specific initiator (I*). This was well designed program four functional modules: I*-recognizable element adopting metastable stem-loop bihairpin structure two splits (exposed C3GT4 locked C4AC4T) aAgNC template that separated by tunable hairpin spacer customized combination selective recognition signaling readout. When presenting I* assay route, hybridization induces directional disassembly unit, on which configurationally switched liberate split. Thus, parent pair C3GT4/C4AC4T proximal, affording situ nucleation clustering emissive aAgNC. By collecting signal, quantitative detection achieved. Benefiting from ingenious programming qMB, recognizing integration actuates construction facile convenient fluorescent biosensor featuring rapid reaction kinetics, wide linear range, high sensitivity, specificity. would provide new paradigm exploit versatile qMB-based biosensing platforms via switches developing various DNA-scaffolded clusters.

Language: Английский

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