Magnetic
nucleic
acid
aptamers
have
superior
molecular
recognition
properties
and
separation
capabilities.
The
aptasensors
demonstrated
excellent
potential
in
the
fields
of
diagnosis
targeted
detection.
Herein,
we
utilized
docking-aided
rational
tailoring
(DART)
strategy
to
analyze
intermolecular
force
interaction
sites
between
zearalenone
(ZEN)
aptamer,
optimize
long-chain
aptamer
step
by
enhance
binding
affinity
3.4
times.
To
address
food
safety
issues
caused
toxins,
established
a
fluorescent
copper
nanocluster
biosensor
based
on
magnetic
for
visual
quantitative
detection
ZEN.
Specifically,
bead-modified
underwent
conformational
changes
when
competing
with
complementary
sequences
bind
Then,
released
will
be
amplified
template-free
mode
presence
terminal
deoxynucleotidyl
transferase
(TdT),
generating
T-rich
as
core
luminescence
nanoclusters.
could
visualized
quantitatively
detected
through
ultraviolet
irradiation.
proposed
label-free
aptasensor
exhibited
high
sensitivity
specificity,
showing
wide
linear
relationship
from
0.1
1000
ng/mL,
low
limit
(LOD)
ng/mL.
Overall,
docking-assisted
optimization
ZEN
provide
insights
into
target
interactions
aptamers.
constructed
fluorescence
also
offers
an
ideal
method
toxin
detection,
bright
prospects
detecting
supply
chain.
Advanced Materials Technologies,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 2, 2024
Abstract
Synthetic
oligonucleic
acids
known
as
aptamers
exhibit
remarkable
selectivity
and
affinity
for
target
recognition
binding.
Selected
via
an
iterative
process
“selective
evolution
of
ligands
by
exponential
enrichment”
(SELEX),
fold
into
defined
3D
conformations
to
interact
with
their
targets.
The
incorporation
elements
has
driven
notable
progress
in
biosensors,
giving
rise
the
development
aptasensors.
Here,
aptamer
discovery
various
types
aptasensors
are
summarized.
fundamental
design
principles
elaborated
along
superiority
compared
antibodies.
modes
employed
aptasensors,
such
structure‐switching
design,
hybridization
chain
reaction
amplification,
enzyme‐assisted
recycling,
split
examined.
Further
light
is
shed
on
diverse
landscape
adaptability
different
analytes
well
potential
propel
advancements
modern
biosensor
technology.
As
a
nucleic
acids‐based
platform,
poise
become
next
generation
sensitive
cost‐effective
technology
shape
future
molecular
biosensing.
Current Topics in Medicinal Chemistry,
Journal Year:
2023,
Volume and Issue:
23(20), P. 1985 - 2000
Published: June 26, 2023
Aptamers,
as
artificially
synthesized
short
nucleotide
sequences,
have
been
widely
used
in
protein
analysis,
gene
engineering,
and
molecular
diagnostics.
Currently,
the
screening
process
of
aptamers
still
relies
on
traditional
SELEX
process,
which
is
cumbersome
complex.
Moreover,
success
rate
aptamer
through
not
high,
has
become
a
major
challenge.
In
recent
years,
development
computers
facilitated
virtual
screening,
can
greatly
accelerate
computer-assisted
screening.
However,
accuracy
precision
current
software
market
vary.
Therefore,
this
work
summarizes
docking
characteristics
four
mainstream
programs,
including
Auto
dock,
dock
Vina,
MOE,
hex
Dock,
years.
prediction
performance
these
programs
for
based
experimental
data
also
evaluated.
This
will
guide
researchers
selection
software.
Additionally,
review
provides
detailed
overview
application
computer-aided
thus
providing
direction
future
field.
Biosensors and Bioelectronics,
Journal Year:
2024,
Volume and Issue:
265, P. 116680 - 116680
Published: Aug. 22, 2024
Aptamers
are
short
oligonucleotides
capable
of
binding
specifically
to
various
targets
(i.e.,
small
molecules,
proteins,
and
whole
cells)
which
have
been
introduced
in
biosensors
such
as
the
electrochemical
aptamer-based
(E-AB)
sensing
platform.
E-AB
sensors
comprised
a
redox-reporter-modified
aptamer
attached
an
electrode
that
undergoes,
upon
target
addition,
binding-induced
change
electron
transfer
rates.
To
date,
faced
limitation
translatability
aptamers
into
platform
presumably
because
sequences
obtained
from
Systematic
Evolution
Ligands
by
Exponential
Enrichment
(SELEX)
typically
long
(>80
nucleotides)
obtaining
structural
information
remains
time
resource
consuming.
In
response,
we
explore
utility
base
truncations
silico
docking
improve
their
sensors.
Here,
first
apply
this
glucose
aptamer,
characterize
solution
using
NMR
methods
guide
design
translate
truncated
variants
biosensors.
We
further
investigated
applicability
truncation
computational
approaches
four
other
systems
(vancomycin,
cocaine,
methotrexate
theophylline)
derived
functional
foresee
our
strategy
will
increase
success
rate
translating
platforms
afford
low-cost
measurements
molecules
directly
undiluted
complex
matrices.
