International Journal of Nanomedicine,
Journal Year:
2023,
Volume and Issue:
Volume 18, P. 6311 - 6331
Published: Nov. 1, 2023
Abstract:
Recent,
outbreaks
of
new
emergency
zoonotic
diseases
have
prompted
an
urgent
need
to
develop
fast,
accurate,
and
portable
screening
assays
for
pathogen
infections.
Recombinase
polymerase
amplification
(RPA)
is
sensitive
specific
can
be
conducted
at
a
constant
low
temperature
with
short
response
time,
making
it
especially
suitable
on-site
powerful
tool
preventing
or
controlling
the
spread
zoonoses.
This
review
summarizes
design
principles
RPA-based
biosensors
as
well
various
signal
output
readout
technologies
involved
in
fluorescence
detection,
lateral
flow
assays,
enzymatic
catalytic
reactions,
spectroscopic
techniques,
electrochemical
chemiluminescence,
nanopore
sequencing
technologies,
microfluidic
digital
RPA,
clustered
regularly
interspaced
palindromic
repeats/CRISPR-associated
systems.
The
current
status
prospects
application
zoonoses
are
highlighted.
demonstrate
advantages
rapid
response,
easy-to-read
result
output,
easy
implementation
enabling
development
toward
greater
portability,
automation,
miniaturization.
Although
there
still
problems
such
high
cost
unstable
increasingly
becoming
one
most
important
means
complex
samples
involving
environmental,
water,
food,
animal,
human
diseases.
Keywords:
recombinase
amplification,
biosensor,
zoonoses,
nanomaterials
ACS Measurement Science Au,
Journal Year:
2023,
Volume and Issue:
3(6), P. 404 - 433
Published: Oct. 27, 2023
The
critical
performance
factors
such
as
selectivity,
sensitivity,
operational
and
storage
stability,
response
time
of
electrochemical
biosensors
are
governed
mainly
by
the
function
their
key
component,
bioelectrode.
Suitable
design
fabrication
strategies
bioelectrode
interface
essential
for
realizing
requisite
practical
utility.
A
multifaceted
attempt
to
achieve
this
goal
is
visible
from
vast
literature
exploring
effective
preparing,
immobilizing,
stabilizing
biorecognition
elements
on
electrode
surface
efficient
transduction
biochemical
signals
into
electrical
ones
(i.e.,
current,
voltage,
impedance)
through
with
aid
advanced
materials
techniques.
commercial
success
in
modern
society
also
increasingly
influenced
size
(and
hence
portability),
multiplexing
capability,
coupling
wireless
communication
technology,
which
facilitates
quick
data
transfer
linked
decision-making
processes
real-time
different
areas
healthcare,
agriculture,
food,
environmental
applications.
Therefore,
involves
careful
selection
control
several
parameters,
including
elements,
materials,
shape
electrode,
detection
principles,
various
strategies,
microscale
printing
technologies.
This
review
discusses
recent
trends
designs
fabrications
developing
biosensors.
discussions
have
been
delineated
types
immobilization
signal
approaches,
commonly
used
techniques
fabricating
bioelectrodes,
device
integration
electronic
technology
interest.
Diagnostics,
Journal Year:
2022,
Volume and Issue:
12(10), P. 2455 - 2455
Published: Oct. 11, 2022
The
CRISPR/Cas
system
is
a
protective
adaptive
immune
against
attacks
from
foreign
mobile
genetic
elements.
Since
the
discovery
of
excellent
target-specific
sequence
recognition
ability
system,
has
shown
performance
in
development
pathogen
nucleic-acid-detection
technology.
In
combination
with
various
biosensing
technologies,
researchers
have
made
many
rapid,
convenient,
and
feasible
innovations
With
an
in-depth
understanding
it
no
longer
limited
to
CRISPR/Cas9,
CRISPR/Cas12,
other
systems
that
had
been
widely
used
past;
families
are
designed
for
nucleic
acid
detection.
We
summarized
application
CRISPR/Cas-related
technology
infectious-disease
detection
its
SARS-CoV-2
BMEMat,
Journal Year:
2023,
Volume and Issue:
1(4)
Published: Sept. 19, 2023
Abstract
Cell
culture
encompasses
procedures
for
extracting
cells
from
their
natural
tissue
and
cultivating
them
under
controlled
artificial
conditions.
During
this
process,
various
factors,
including
cell
physiological/morphological
properties,
environments,
metabolites,
contaminants,
have
to
be
precisely
monitored
the
survival
of
pursuit
desired
properties
cells.
This
review
summarizes
recent
advances
in
sensor
technologies
manufacturing
strategies
platforms
using
traditional
plastics,
microfluidic
chips,
scalable
bioreactors.
We
share
details
newly
developed
biological
sensors,
chemical
optical
electronic
chip
technologies,
material
integration
methods.
The
precise
control
parameters
based
on
feedback
by
these
sensors
electronics
enhances
quality
throughput.
Biosensors and Bioelectronics,
Journal Year:
2024,
Volume and Issue:
258, P. 116340 - 116340
Published: April 25, 2024
The
escalating
global
incidence
of
infectious
diseases
caused
by
pathogenic
bacteria,
especially
in
developing
countries,
emphasises
the
urgent
need
for
rapid
and
portable
pathogen
detection
devices.
This
study
introduces
a
sensitive
specific
electrochemical
biosensing
platform
utilising
cost-effective
electrodes
fabricated
inkjet-printing
gold
silver
nanoparticles
on
plastic
substrate.
biosensor
exploits
CRISPR/Cas12a
system
detecting
DNA
sequence
selected
from
genome
target
pathogen.
Upon
detection,
trans-activity
Cas12a/gRNA
is
triggered,
leading
to
cleavage
rationally
designed
single-strand
reporters
(linear
hairpin)
labelled
with
methylene
blue
(ssDNA-MB)
bound
electrode
surface.
In
principle,
this
sensing
mechanism
can
be
adapted
any
bacterium
choosing
proper
guide
RNA
its
DNA.
biosensor's
performance
was
assessed
two
representative
pathogens
(a
Gram-negative,
Escherichia
coli,
Gram-positive,
Staphylococcus
aureus),
results
obtained
inkjet-printed
were
compared
those
commercial
screen-printed
electrodes.
Our
show
that
use
nanostructured
electrodes,
which
provide
large
surface
area,
combination
hairpin
containing
poly-T
loop
increase
sensitivity
assay
corresponding
signal
variation
86%.
targets
amplified
various
clinically
isolated
have
been
tested
demonstrate
potential
proposed
point-of-need
applications.
