Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening DOI Creative Commons
X. Feng,

Linyin Yan,

Yang Zhao

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 6311 - 6331

Published: Nov. 1, 2023

Abstract: Recent, outbreaks of new emergency zoonotic diseases have prompted an urgent need to develop fast, accurate, and portable screening assays for pathogen infections. Recombinase polymerase amplification (RPA) is sensitive specific can be conducted at a constant low temperature with short response time, making it especially suitable on-site powerful tool preventing or controlling the spread zoonoses. This review summarizes design principles RPA-based biosensors as well various signal output readout technologies involved in fluorescence detection, lateral flow assays, enzymatic catalytic reactions, spectroscopic techniques, electrochemical chemiluminescence, nanopore sequencing technologies, microfluidic digital RPA, clustered regularly interspaced palindromic repeats/CRISPR-associated systems. The current status prospects application zoonoses are highlighted. demonstrate advantages rapid response, easy-to-read result output, easy implementation enabling development toward greater portability, automation, miniaturization. Although there still problems such high cost unstable increasingly becoming one most important means complex samples involving environmental, water, food, animal, human diseases. Keywords: recombinase amplification, biosensor, zoonoses, nanomaterials

Language: Английский

Advances in Bioelectrode Design for Developing Electrochemical Biosensors DOI Creative Commons

Nabajyoti Kalita,

Sudarshan Gogoi, Shelley D. Minteer

et al.

ACS Measurement Science Au, Journal Year: 2023, Volume and Issue: 3(6), P. 404 - 433

Published: Oct. 27, 2023

The critical performance factors such as selectivity, sensitivity, operational and storage stability, response time of electrochemical biosensors are governed mainly by the function their key component, bioelectrode. Suitable design fabrication strategies bioelectrode interface essential for realizing requisite practical utility. A multifaceted attempt to achieve this goal is visible from vast literature exploring effective preparing, immobilizing, stabilizing biorecognition elements on electrode surface efficient transduction biochemical signals into electrical ones (i.e., current, voltage, impedance) through with aid advanced materials techniques. commercial success in modern society also increasingly influenced size (and hence portability), multiplexing capability, coupling wireless communication technology, which facilitates quick data transfer linked decision-making processes real-time different areas healthcare, agriculture, food, environmental applications. Therefore, involves careful selection control several parameters, including elements, materials, shape electrode, detection principles, various strategies, microscale printing technologies. This review discusses recent trends designs fabrications developing biosensors. discussions have been delineated types immobilization signal approaches, commonly used techniques fabricating bioelectrodes, device integration electronic technology interest.

Language: Английский

Citations

20

A label-free aptasensor for colorimetric detection of food toxin: Mediation of catalytically active gold nanozymes and smartphone imaging strategy DOI
Zahra Khoshbin, Marzieh Moeenfard, Khalil Abnous

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 433, P. 137355 - 137355

Published: Sept. 3, 2023

Language: Английский

Citations

18

A supersensitive electrochemical sensor based on RCA amplification-assisted “silver chain”-linked gold interdigital electrodes and CRISPR/Cas9 for the detection of Staphylococcus aureus in food DOI
Deshuai Zhen, Shaoqi Zhang,

Aofeng Yang

et al.

Food Chemistry, Journal Year: 2023, Volume and Issue: 440, P. 138197 - 138197

Published: Dec. 14, 2023

Language: Английский

Citations

18

CRISPR/Cas biosensing technology: From lab assays to integrated portable devices towards wearables DOI

Ruya Shi,

Ling Zhong, Guozhen Liu

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 177, P. 117796 - 117796

Published: June 2, 2024

Language: Английский

Citations

7

Application of CRISPR/Cas Systems in the Nucleic Acid Detection of Infectious Diseases DOI Creative Commons
Junwei Li, Yuexia Wang, Bin Wang

et al.

Diagnostics, Journal Year: 2022, Volume and Issue: 12(10), P. 2455 - 2455

Published: Oct. 11, 2022

The CRISPR/Cas system is a protective adaptive immune against attacks from foreign mobile genetic elements. Since the discovery of excellent target-specific sequence recognition ability system, has shown performance in development pathogen nucleic-acid-detection technology. In combination with various biosensing technologies, researchers have made many rapid, convenient, and feasible innovations With an in-depth understanding it no longer limited to CRISPR/Cas9, CRISPR/Cas12, other systems that had been widely used past; families are designed for nucleic acid detection. We summarized application CRISPR/Cas-related technology infectious-disease detection its SARS-CoV-2

Language: Английский

Citations

26

Advances in sensor developments for cell culture monitoring DOI Creative Commons
Ka Ram Kim, Woon‐Hong Yeo

BMEMat, Journal Year: 2023, Volume and Issue: 1(4)

Published: Sept. 19, 2023

Abstract Cell culture encompasses procedures for extracting cells from their natural tissue and cultivating them under controlled artificial conditions. During this process, various factors, including cell physiological/morphological properties, environments, metabolites, contaminants, have to be precisely monitored the survival of pursuit desired properties cells. This review summarizes recent advances in sensor technologies manufacturing strategies platforms using traditional plastics, microfluidic chips, scalable bioreactors. We share details newly developed biological sensors, chemical optical electronic chip technologies, material integration methods. The precise control parameters based on feedback by these sensors electronics enhances quality throughput.

Language: Английский

Citations

14

Recent advances of nanoparticles-assisted CRISPR/Cas biosensors DOI
Sitong Liu, Xu Li,

Zhaohe Huang

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 199, P. 109930 - 109930

Published: Jan. 9, 2024

Language: Английский

Citations

6

Low-cost inkjet-printed nanostructured biosensor based on CRISPR/Cas12a system for pathogen detection DOI Creative Commons
Angela Gilda Carota, Andrea Bonini, Massimo Urban

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 258, P. 116340 - 116340

Published: April 25, 2024

The escalating global incidence of infectious diseases caused by pathogenic bacteria, especially in developing countries, emphasises the urgent need for rapid and portable pathogen detection devices. This study introduces a sensitive specific electrochemical biosensing platform utilising cost-effective electrodes fabricated inkjet-printing gold silver nanoparticles on plastic substrate. biosensor exploits CRISPR/Cas12a system detecting DNA sequence selected from genome target pathogen. Upon detection, trans-activity Cas12a/gRNA is triggered, leading to cleavage rationally designed single-strand reporters (linear hairpin) labelled with methylene blue (ssDNA-MB) bound electrode surface. In principle, this sensing mechanism can be adapted any bacterium choosing proper guide RNA its DNA. biosensor's performance was assessed two representative pathogens (a Gram-negative, Escherichia coli, Gram-positive, Staphylococcus aureus), results obtained inkjet-printed were compared those commercial screen-printed electrodes. Our show that use nanostructured electrodes, which provide large surface area, combination hairpin containing poly-T loop increase sensitivity assay corresponding signal variation 86%. targets amplified various clinically isolated have been tested demonstrate potential proposed point-of-need applications.

Language: Английский

Citations

6

Magnetic particles-integrated CRISPR/Cas systems for biosensing DOI
Zhilong Wang,

Luyu Wei,

Yiping Chen

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 171, P. 117525 - 117525

Published: Jan. 4, 2024

Language: Английский

Citations

5

Stimuli-responsive incremental DNA machine auto-catalyzed CRISPR-Cas12a feedback amplification permits ultrasensitive molecular diagnosis of esophageal cancer-related microRNA DOI
Hongxia Li, Yi Wang,

Yu Wan

et al.

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125675 - 125675

Published: Jan. 21, 2024

Language: Английский

Citations

5