Label-free, real-time monitoring of cytochrome C drug responses in microdissected tumor biopsies with a multi-well aptasensor platform DOI Creative Commons
Tran N. H. Nguyen, Lisa F. Horowitz,

Timothy Krilov

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(36)

Published: Sept. 6, 2024

Functional assays on intact tumor biopsies can complement genomics-based approaches for precision oncology, drug testing, and organs-on-chips cancer disease models by capturing key therapeutic response determinants, such as tissue architecture, heterogeneity, the microenvironment. Most of these rely fluorescent labeling, a semiquantitative method best suited single-time-point or labor-intensive immunostaining analysis. Here, we report integrated aptamer electrochemical sensors on-chip, real-time monitoring cytochrome C, cell death indicator, from microdissected tissues with high affinity specificity. The platform features multi-well sensor layout multiplexed electronic setup. aptasensors measure increases in C supernatant mouse human tumors after exposure to various treatments. Because sensor’s affinity, it primarily tracks rising concentrations dynamic changes during apoptosis. This approach could help develop more advanced apply other complex vitro models, organoids.

Language: Английский

Multimaterial 3D and 4D Bioprinting of Heterogenous Constructs for Tissue Engineering DOI
Annan Chen, Wanying Wang, Zhengyi Mao

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(34)

Published: Sept. 22, 2023

Additive manufacturing (AM), which is based on the principle of layer-by-layer shaping and stacking discrete materials, has shown significant benefits in fabrication complicated implants for tissue engineering (TE). However, many native tissues exhibit anisotropic heterogenous constructs with diverse components functions. Consequently, replication biomimetic using conventional AM processes a single material challenging. Multimaterial 3D 4D bioprinting (with time as fourth dimension) emerged promising solution constructing multifunctional that can mimic host microenvironment better than single-material alternatives. Notably, 4D-printed multimaterial architectures provide time-dependent programmable dynamic promote cell activity regeneration response to external stimuli. This paper first presents typical design strategies TE applications. Subsequently, latest are discussed, along their advantages challenges. In particular, potential smart highlighted. Furthermore, this review provides insights into how facilitate realization next-generation

Language: Английский

Citations

65
A review of the recent achievements and future trends on 3D printed microfluidic devices for bioanalytical applications DOI
Lucas C. Duarte, Federico Figueredo, C Chagas

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1299, P. 342429 - 342429

Published: Feb. 28, 2024

Language: Английский

Citations

16
From Organ-on-a-Chip to Human-on-a-Chip: A Review of Research Progress and Latest Applications DOI

Yisha Huang,

Tong Liu,

Qi Huang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(7), P. 3466 - 3488

Published: July 11, 2024

Organ-on-a-Chip (OOC) technology, which emulates the physiological environment and functionality of human organs on a microfluidic chip, is undergoing significant technological advancements. Despite its rapid evolution, this technology also facing notable challenges, such as lack vascularization, development multiorgan-on-a-chip systems, replication body single chip. The progress has played crucial role in steering OOC toward mimicking microenvironment, including microenvironment replication, multiorgan microphysiological systems. Additionally, advancements detection, analysis, organoid imaging technologies have enhanced efficiency Organs-on-Chips (OOCs). In particular, integration artificial intelligence revolutionized imaging, significantly enhancing high-throughput drug screening. Consequently, review covers research Human-on-a-chip, sensors OOCs, latest applications biomedical field.

Language: Английский

Citations

15
Harnessing the power of artificial intelligence for human living organoid research DOI Creative Commons
Hui Wang, Xiangyang Li, Xiaoyan You

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 140 - 164

Published: Aug. 30, 2024

As a powerful paradigm, artificial intelligence (AI) is rapidly impacting every aspect of our day-to-day life and scientific research through interdisciplinary transformations. Living human organoids (LOs) have great potential for

Language: Английский

Citations

11
Recent advances in Organ-on-a-Chip models: How precision engineering integrates cutting edge technologies in fabrication and characterization DOI
Sorour Sadeghzade, Mohammad Hosseini Hooshiar, Hossein Akbari

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 38, P. 102231 - 102231

Published: May 14, 2024

Language: Английский

Citations

9
Biosensors in Biomedical Research: Bridging Cell and Tissue Engineering and Real-Time Monitoring DOI
Zahra Rezaei, Niyou Wang, A. Rodríguez

et al.

Current Opinion in Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 100582 - 100582

Published: Feb. 1, 2025

Language: Английский

Citations

1
From organoids to organoids-on-a-chip: Current applications and challenges in biomedical research DOI Creative Commons
Kailun Liu,

Xiaowei Chen,

Zhen Fan

et al.

Chinese Medical Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Abstract The high failure rates in clinical drug development based on animal models highlight the urgent need for more representative human biomedical research. In response to this demand, organoids and organ chips were integrated greater physiological relevance dynamic, controlled experimental conditions. This innovative platform—the organoids-on-a-chip technology—shows great promise disease modeling, discovery, personalized medicine, attracting interest from researchers, clinicians, regulatory authorities, industry stakeholders. review traces evolution organoids-on-a-chip, driven by necessity advanced biological models. We summarize applications of simulating pathological phenotypes therapeutic evaluation technology. section highlights how integrating technologies chips, such as microfluidic systems, mechanical stimulation, sensor integration, optimizes organoid cell types, spatial structure, functions, thereby expanding their applications. conclude addressing current challenges offering insights into prospects. advancement is poised enhance fidelity, standardization, scalability. Furthermore, integration cutting-edge interdisciplinary collaborations will be crucial progression

Language: Английский

Citations

1
Organ-on-a-chip platforms integrated with biosensors for precise monitoring of the cells and cellular microenvironment DOI
Weiyou Yang,

Tengyun Li,

Shufei Liao

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117569 - 117569

Published: Jan. 30, 2024

Language: Английский

Citations

8
An Insight on Microfluidic Organ-on-a-Chip Models for PM2.5-Induced Pulmonary Complications DOI Creative Commons
D. O. Shah, Bhavarth P. Dave, Mehul R. Chorawala

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(12), P. 13534 - 13555

Published: March 7, 2024

Pulmonary diseases like asthma, chronic obstructive pulmonary disorder, lung fibrosis, and cancer pose a significant burden to global human health. Many of these complications arise as result exposure particulate matter (PM), which has been examined in several preclinical clinical trials for its effect on respiratory diseases. Particulate size less than 2.5 μm (PM2.5) known inflict unforeseen repercussions, although data from epidemiological studies back this are pending. Conventionally utilized two-dimensional (2D) cell culture animal models have provided insufficient benefits emulating the vivo physiological pathological conditions. Three-dimensional (3D) structural models, including organ-on-a-chip experienced developmental upsurge recent times. Lung-on-a-chip potential simulate specific features lungs. With advancement technology, an emerging advanced technique termed microfluidic developed with aim identifying complexity cellular microenvironment body. In present Review, role lung-on-a-chip modeling reproducing explored, emphasis PM2.5-induced complications.

Language: Английский

Citations

6
Using Biosensors to Study Organoids, Spheroids and Organs-on-a-Chip: A Mechanobiology Perspective DOI Creative Commons
Muhammad Sulaiman Yousafzai, John A. Hammer

Biosensors, Journal Year: 2023, Volume and Issue: 13(10), P. 905 - 905

Published: Sept. 24, 2023

The increasing popularity of 3D cell culture models is being driven by the demand for more in vivo-like conditions with which to study biochemistry and biomechanics numerous biological processes health disease. Spheroids organoids are platforms that self-assemble regenerate from stem cells, tissue progenitor cells or lines, show great potential studying development regeneration. Organ-on-a-chip approaches can be used achieve spatiotemporal control over biochemical biomechanical signals promote growth differentiation. These model systems engineered serve as disease drug screens. While methods have been developed support these structures, challenges remain completely recapitulate cell–cell cell–matrix interactions occurring vivo. Understanding how forces influence functions will require precise tools measure such forces, well a better understanding mechanobiology interactions. Biosensors prove powerful measuring both contexts, thereby leading mechanical at cellular levels. Here, we discussed biosensors mechanobiological research coupled develop accurate, physiologically relevant development, function, malfunction disease, avenues intervention.

Language: Английский

Citations

13