Cascade nucleic acid amplification–assisted CRISPR/Cas12a electrochemiluminescence biosensor using C3N4 nanomaterials for sensitive detection of MicroRNA-320d DOI
Lina Zhang,

Zuhao Zhang,

Zheng Yang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 426, P. 137140 - 137140

Published: Dec. 18, 2024

Language: Английский

Rapid and accurate SERS assay of disease-related nucleic acids based on isothermal cascade signal amplifications of CRISPR/Cas13a system and catalytic hairpin assembly DOI
Jingjing Zhang,

Zhilong Chen,

Huiming Lv

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 253, P. 116196 - 116196

Published: March 6, 2024

Language: Английский

Citations

9
Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors DOI Creative Commons

Xiaonan Xin,

Jing Su, Haoran Cui

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 155 - 155

Published: March 2, 2025

High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, environmental monitoring. CRISPR/Cas systems have emerged as transformative tools biosensing due to their unparalleled specificity, programmability, unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target guided by specific protospacers, which ensures accurate target recognition, bystander activity triggered upon binding, enables robust signal amplification. These properties make highly versatile designing biosensors ultra-sensitive detection. This review comprehensively explores recent advancements system-based biosensors, highlighting impact on improving performance. We discuss the integration of with diverse readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), so on. Additionally, we examine development integrated systems, such microfluidic devices portable leverage technology point-of-care testing (POCT) high-throughput analysis. Furthermore, identify unresolved challenges, aiming inspire innovative solutions accelerate translation these technologies into practical diagnostics, food, environment safety.

Language: Английский

Citations

0
High-fidelity telomerase activity assay based on light-triggered nucleic acid separation system for the diagnosis of bladder cancer DOI

Meng Cheng,

Ligang Zhang,

Xiujing Han

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117355 - 117355

Published: March 7, 2025

Language: Английский

Citations

0
Polydopamine Powered Droplet Electricity Generator for Protein Assay with CRISPR/Cas Enabled Amplification DOI Creative Commons
Xiaolin Qu,

Yuyan Yue,

Yuguo Tang

et al.

Nano Letters, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Protein function investigation and clinical assay are fundamental to modern biology medical diagnostics. Flap endonuclease 1 (FEN1), a key enzyme in DNA replication repair, plays critical role the progression of many diseases. Taking FEN1 as an example, we present novel protein detection platform combining triboelectric nanogenerator (TENG) CRISPR/Cas technologies. As specific form TENG, transistor-droplet electricity generator (TDEG) is explored, which offers low-cost, simple fabrication approach with real-time capability. Meanwhile, activated system catalyzes reactions on three-dimensional tetrahedron interface, promising high sensitivity. This work not only demonstrates powerful method for rapid but also pioneers integration TENG. It has great prospect future development TENG sensors.

Language: Английский

Citations

0
Dual‐Mode Detection of RNA Demethylation Protein ALKBH3 Using a Two‐Legged DNA‐Walker‐Mediated BiOI/In2O3‐Gated Organic Photoelectrochemical Transistor Biosensor DOI
Yixin Hu, Yunlei Zhou, Huanshun Yin

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Abstract Organic photoelectrochemical transistor (OPECT) biosensors have emerged as prevalent candidates for bioanalytical technologies because of their intrinsic amplification properties and excellent detection performance. Herein, a novel OPECT biosensor analyzing the RNA demethylation protein ALKBH3 is developed. A BiOI/In 2 O 3 type‐II heterojunction employed photoactive material gate, an organic semiconductor, poly(3,4‐ethylenedioxythiophene): poly(styrenesulfonic) (PEDOT:PSS), used channel modification material. ALKBH3‐mediated demethylation, switch, triggered trans‐cleavage activity CRISPR‐Cas12a system, which subsequently leads to failure in formation bipedal DNA walker. Contrarily, ferrocenecarboxylic acid (FcA), acts effective electron donor, cannot be captured, leading modulation gating characteristics degradation current response. Compared conventional method, exhibits higher sensitivity lower limit protein, thereby facilitating monitoring changes under effect environmental pollutants demonstrating crucial biomarker potential evaluating its toxicological effects.

Language: Английский

Citations

0
A small library crRNA-enhanced CRISPR-Cas12a system for ultrasensitive point-of-care test of hantavirus M gene DOI Creative Commons
Jian Zhou, Xuemei Ren, Xin Wang

et al.

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100811 - 100811

Published: May 1, 2025

Language: Английский

Citations

0
CRISPR-Based Regulation for High-Throughput Screening DOI

Lingling Jiao,

Qi Zhou, Dongchang Sun

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

CRISPR technology has revolutionized genome editing by enabling precise, permanent modifications to genetic material. To circumvent the irreversible alterations associated with traditional methods and facilitate research on both essential nonessential genes, interference or inhibition (CRISPRi) activation (CRISPRa) were developed. The gene-silencing approach leverages an inactivated Cas effector protein paired guide RNA obstruct transcription initiation elongation, while gene-activation exploits programmability of activate gene expression. Recent advances in CRISPRi technology, combination other technologies (e.g., biosensing, sequencing), have significantly expanded its applications, allowing for genome-wide high-throughput screening (HTS) identify determinants phenotypes. These strategies been applied biomedicine, industry, basic research. This review explores regulation mechanisms, offers overview workflow CRISPR-based screens, highlights superior suitability HTS across biomedical industrial applications. Finally, we discuss limitations current CRISPRi/a envision future directions CRISPR-mediated research, considering potential broader application diverse fields.

Language: Английский

Citations

0
Development of a Multi-miRNA Detection Platform for Enhanced Diagnostics of Colorectal Cancer DOI
Bo Zhou, Tingting Fan, Hui Chen

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117639 - 117639

Published: May 1, 2025

Language: Английский

Citations

0
CRISPR/HCR-powered ratiometric fluorescence aptasensor for ochratoxin A detection DOI
Xiaokun Wang, Hao Ding,

Yinghui Sun

et al.

Food Chemistry, Journal Year: 2024, Volume and Issue: 468, P. 142437 - 142437

Published: Dec. 12, 2024

Language: Английский

Citations

2
Cascade nucleic acid amplification–assisted CRISPR/Cas12a electrochemiluminescence biosensor using C3N4 nanomaterials for sensitive detection of MicroRNA-320d DOI
Lina Zhang,

Zuhao Zhang,

Zheng Yang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 426, P. 137140 - 137140

Published: Dec. 18, 2024

Language: Английский

Citations

2