Polyextremophile engineering: a review of organisms that push the limits of life DOI Creative Commons
Joaquín Caro‐Astorga, Joseph T. Meyerowitz, Devon A. Stork

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: June 5, 2024

Nature exhibits an enormous diversity of organisms that thrive in extreme environments. From snow algae reproduce at sub-zero temperatures to radiotrophic fungi nuclear radiation Chernobyl, raise many questions about the limits life. Is there any environment where life could not “find a way”? Although individual extremophilic have been identified and studied, remain outstanding extent which properties can be enhanced, combined or transferred new organisms. In this review, we compile current knowledge on bioengineering extremophile microbes. We summarize what is known basic mechanisms adaptations, synthetic biology’s efforts engineer beyond found nature, highlight adaptations combined. The science extremophiles applied engineered tailored specific biomanufacturing needs, such as growth high presence unusual solvents.

Language: Английский

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production DOI Creative Commons
Mafalda Trovão, Lisa Schüler, Adriana Rodrigues Machado

et al.

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(7), P. 440 - 440

Published: June 30, 2022

Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand food feed. However, microalgae-based products are currently hindered by high production costs. One major reason this is that commonly cultivated wildtype strains do not possess robustness productivity required successful industrial production. Several strain improvement technologies been developed towards creating more stress tolerant productive strains. While classical methods of forward genetics extensively used to determine gene function randomly generated mutants, reverse has explored generate specific mutations target phenotypes. Site-directed mutagenesis can be accomplished employing different editing tools, which enable generation tailor-made genotypes. Nevertheless, strategies promoting selection mutants avoid introduction foreign genetic material. In paper, we review microalgal approaches their applications, with primary focus on random mutagenesis. Current challenges hampering improvement, selection, commercialization will discussed. The combination these high-throughput technologies, such as fluorescence-activated cell sorting, tools select most also

Language: Английский

Citations

80
Engineering of Saccharomyces cerevisiae as a consolidated bioprocessing host to produce cellulosic ethanol: Recent advancements and current challenges DOI

Juhi Sharma,

Vinod Kumar, Rajendra Prasad

et al.

Biotechnology Advances, Journal Year: 2022, Volume and Issue: 56, P. 107925 - 107925

Published: Feb. 10, 2022

Language: Английский

Citations

75
Potential of microalgae cultivation using nutrient-rich wastewater and harvesting performance by biocoagulants/bioflocculants: Mechanism, multi-conversion of biomass into valuable products, and future challenges DOI
Setyo Budi Kurniawan, Azmi Ahmad, Muhammad Fauzul Imron

et al.

Journal of Cleaner Production, Journal Year: 2022, Volume and Issue: 365, P. 132806 - 132806

Published: June 21, 2022

Language: Английский

Citations

72
Biosynthesis of monoterpenoid and sesquiterpenoid as natural flavors and fragrances DOI
Hui Jiang, Xi Wang

Biotechnology Advances, Journal Year: 2023, Volume and Issue: 65, P. 108151 - 108151

Published: April 8, 2023

Language: Английский

Citations

48
Automated in vivo enzyme engineering accelerates biocatalyst optimization DOI Creative Commons
Enrico Orsi, Lennart Schada von Borzyskowski, Stephan Noack

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 24, 2024

Achieving cost-competitive bio-based processes requires development of stable and selective biocatalysts. Their realization through in vitro enzyme characterization engineering is mostly low throughput labor-intensive. Therefore, strategies for increasing while diminishing manual labor are gaining momentum, such as vivo screening evolution campaigns. Computational tools like machine learning further support efforts by widening the explorable design space. Here, we propose an integrated solution to challenges whereby ML-guided, automated workflows (including library generation, implementation hypermutation systems, adapted laboratory evolution, growth-coupled selection) could be realized accelerate pipelines towards superior

Language: Английский

Citations

26
Bioengineering in Solid-State Fermentation for next sustainable food bioprocessing DOI Creative Commons
Muyideen Olaitan Bamidele, Micheal Bola Bamikale, Eliseo Cárdenas-Hernández

et al.

Next Sustainability, Journal Year: 2025, Volume and Issue: 6, P. 100105 - 100105

Published: Jan. 1, 2025

Language: Английский

Citations

6
Enhanced sustainable integration of CO2 utilization and wastewater treatment using microalgae in circular economy concept DOI
Junhui Chen,

Leilei Dai,

Dmitri Mataya

et al.

Bioresource Technology, Journal Year: 2022, Volume and Issue: 366, P. 128188 - 128188

Published: Oct. 26, 2022

Language: Английский

Citations

55
Optimization and Process Effect for Microalgae Carbon Dioxide Fixation Technology Applications Based on Carbon Capture: A Comprehensive Review DOI Creative Commons
Gang Li,

Wenbo Xiao,

Tenglun Yang

et al.

C – Journal of Carbon Research, Journal Year: 2023, Volume and Issue: 9(1), P. 35 - 35

Published: March 16, 2023

Microalgae carbon dioxide (CO2) fixation technology is among the effective ways of environmental protection and resource utilization, which can be combined with treatment wastewater flue gas, preparation biofuels other technologies, high economic benefits. However, in industrial application, microalgae still have problems such as poor photosynthetic efficiency, input cost large capital investment. The energy development utilization needs to further studied. Therefore, this work reviewed mechanism CO2 microalgae. Improving sequestration capacity by adjusting parameters their growth conditions (e.g., light, temperature, pH, nutrient elements, concentration) was briefly discussed. strategies random mutagenesis, adaptive laboratory evolution genetic engineering were evaluated screen a rate, strong tolerance, efficiency biomass. In addition, order better realize industrialization technology, feasibility combining gas utilizing high-value-added products analyzed. Considering current challenges application above aspects expected establish more optimized future. At same time, it provides solid foundation favorable basis for fully implementing sustainable development, steadily promoting peak neutrality, realizing clean, green, low-carbon efficient energy.

Language: Английский

Citations

32
Biotechnological production of omega-3 fatty acids: current status and future perspectives DOI Creative Commons

Jiansong Qin,

Elif Kurt,

Tyler LBassi

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 7, 2023

Omega-3 fatty acids, including alpha-linolenic acids (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic (DHA), have shown major health benefits, but the human body’s inability to synthesize them has led necessity of dietary intake products. The omega-3 market grown significantly, with a global from an estimated USD 2.10 billion in 2020 predicted nearly 3.61 2028. However, obtaining sufficient supply high-quality stable can be challenging. Currently, fish oil serves as primary source market, it several drawbacks, high cost, inconsistent product quality, uncertainties its sustainability ecological impact. Other significant sources include plants microalgae fermentation, they face similar challenges reducing manufacturing costs improving quality sustainability. With advances synthetic biology, biotechnological production via engineered microbial cell factories still offers best solution provide more stable, sustainable, affordable by overcoming issues associated conventional sources. This review summarizes current status, key challenges, future perspectives for acids.

Language: Английский

Citations

28
Photodriven Chemical Synthesis by Whole-Cell-Based Biohybrid Systems: From System Construction to Mechanism Study DOI

Jiayuan Shen,

Yun Liu, Liang Qiao

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(5), P. 6235 - 6259

Published: Jan. 26, 2023

By simulating natural photosynthesis, the desirable high-value chemical products and clean fuels can be sustainably generated with solar energy. Whole-cell-based photosensitized biohybrid system, which innovatively couples excellent light-harvesting capacity of semiconductor materials efficient catalytic ability intracellular biocatalysts, is an appealing interdisciplinary creature to realize photodriven synthesis. In this review, we summarize constructed whole-cell-based systems in different application fields, including carbon dioxide fixation, nitrogen hydrogen production, other Moreover, elaborate charge transfer mechanism studies representative biohybrids, help deepen current understanding synergistic process between photosensitizers microorganisms, provide schemes for building novel biohybrids less electron resistance, advanced productive efficiency, functional diversity. Further exploration field has prospect making a breakthrough on biotic–abiotic interface that will opportunities multidisciplinary research.

Language: Английский

Citations

24