Adaptive laboratory evolution to obtain furfural tolerant Saccharomyces cerevisiae for bioethanol production and the underlying mechanism DOI Creative Commons

Lan Yao,

Youpiao Jia,

Qingyan Zhang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Jan. 4, 2024

Introduction Furfural, a main inhibitor produced during pretreatment of lignocellulose, has shown inhibitory effects on S. cerevisiae . Method In the present study, new strains named 12–1 with enhanced resistance to furfural were obtained through adaptive laboratory evolution, which exhibited shortened lag phase by 36 h, and an increased ethanol conversion rate 6.67% under 4 g/L furfural. Results Discussion To further explore mechanism tolerance, ADR1_1802 mutant was constructed CRISPR/Cas9 technology, based whole genome re-sequencing data. The results indicated that time when begin grow 20 h compared reference strain ( CEN.PK113-5D) g/L. Additionally, transcription levels GRE2 ADH6 in ADR1_ 1802 53.69 44.95%, respectively, according real-time fluorescence quantitative PCR analysis. These findings suggest tolerance is due accelerated degradation. Importance: Renewable carbon worldwide vital achieve “zero carbon” target. Bioethanol from biomass one them. make bioethanol price competitive fossil fuel, higher yield necessary, therefore, monosaccharide should be effectively converted Saccharomyces However, inhibitors formed glucose or xylose oxidation could lower. Thus, tolerant important this process. As component hydrolysate, shows obvious impact growth production get find underlying mechanism, evolution technology applied study

Language: Английский

Technoeconomic and environmental perspectives of biofuel production from sugarcane bagasse: Current status, challenges and future outlook DOI

Shiyou Pan,

Hossain M. Zabed, Yutuo Wei

et al.

Industrial Crops and Products, Journal Year: 2022, Volume and Issue: 188, P. 115684 - 115684

Published: Sept. 26, 2022

Language: Английский

Citations

74
Microbial cellulase production and its potential application for textile industries DOI Creative Commons
Gamachis Korsa, Rocktotpal Konwarh, Chandran Masi

et al.

Annals of Microbiology, Journal Year: 2023, Volume and Issue: 73(1)

Published: April 10, 2023

Abstract Purpose The textile industry’s previous chemical use resulted in thousands of practical particulate emissions, such as machine component damage and drainage system blockage, both which have implications. Enzyme-based processing is cost-effective, environmentally friendly, non-hazardous, water-saving. purpose this review to give evidence on the potential activity microbial cellulase industry, mostly confined realm research. Methods This was progressive by considering peer-reviewed papers linked production, its prospective application for industries appraised produced develop assessment. Articles were divided into two categories based results trustworthy educational journals: methods used produce diversity microorganisms through fermentation processes approaches microbes fermentation. Submerged (SMF) solid-state (SSF) techniques are currently being meet industrial demand production bio industry. Results Microbial vital increasing day due no side effect environment human health becoming increasingly important. In conventional processing, gray cloth subjected a series treatments that involved breaking dye molecule’s amino group with Cl − , started accelerated dye(-resistant) bond cracking. A enzyme primarily derived from variety species found various ecological settings biotextile/bio-based product technology future needs applications. Conclusion Cellulase has been advantages cellulose-based textiles, well quality enhancement fabric maintenance over traditional approaches. Cellulase’s role industry chosen an appropriate sound solution long healthy lifestyle.

Language: Английский

Citations

58
Big data mining, rational modification, and ancestral sequence reconstruction inferred multiple xylose isomerases for biorefinery DOI Creative Commons
Sitong Chen, Zhaoxian Xu,

Boning Ding

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(5)

Published: Feb. 1, 2023

The isomerization of xylose to xylulose is considered the most promising approach initiate bioconversion. Here, phylogeny-guided big data mining, rational modification, and ancestral sequence reconstruction strategies were implemented explore new active isomerases (XIs) for Saccharomyces cerevisiae. Significantly, 13 XIs S. cerevisiae mined or artificially created. Moreover, importance amino-terminal fragment maintaining basic XI activity was demonstrated. With XIs, four efficient xylose-utilizing constructed evolved, among which strain CRD5HS contributed ethanol titers as high 85.95 94.76 g/liter from pretreated corn stover cob, respectively, without detoxifying washing biomass. Potential genetic targets obtained adaptive laboratory evolution further analyzed by sequencing high-performance strains. combined mining methods described here provide practical references other scarce valuable enzymes.

Language: Английский

Citations

51
A review on polyhydroxyalkanoate (PHA) production through the use of lignocellulosic biomass DOI Creative Commons

Peter Zytner,

Deepak Kumar, Abdallah M. Elsayed

et al.

RSC Sustainability, Journal Year: 2023, Volume and Issue: 1(9), P. 2120 - 2134

Published: Jan. 1, 2023

This work examines the process of using lignocellulosic biomass for cost-effective polyhydroxyalkanoate production, from pre-treatment to extraction.

Language: Английский

Citations

46
Rice straw for energy and value-added products in China: a review DOI Creative Commons
Ahmed Alengebawy, Ran Yi, Nirmal Ghimire

et al.

Environmental Chemistry Letters, Journal Year: 2023, Volume and Issue: 21(5), P. 2729 - 2760

Published: June 15, 2023

Abstract The rise of global waste and the decline fossil fuels are calling for recycling into energy materials. For example, rice straw, a by-product cultivation, can be converted biogas by-products with added value, e.g., biofertilizer, yet processing straw is limited by low content, high ash silica, nitrogen, moisture, high-quality variability. Here, we review focus on Chinese situations, conversion gas, digestate management, cogeneration, upgrading, bioeconomy, life cycle assessment. quality improved pretreatments, such as baling, ensiling, co-digestion other feedstocks. used to fertilize soils. average annual potential collectable lower heating value 15.35 megajoule/kilogram, over past ten years (2013–2022) could reach 2.41 × 10 9 megajoule.

Language: Английский

Citations

44
Research advances on the consolidated bioprocessing of lignocellulosic biomass DOI Creative Commons

Zhongye Li,

Pankajkumar R. Waghmare, Lubbert Dijkhuizen

et al.

Engineering Microbiology, Journal Year: 2024, Volume and Issue: 4(2), P. 100139 - 100139

Published: Feb. 2, 2024

Lignocellulosic biomass is an abundant and renewable bioresource for the production of biofuels biochemical products. The classical biorefinery process lignocellulosic degradation conversion comprises three stages, i.e., pretreatment, enzymatic saccharification, fermentation. However, complicated pretreatment process, high cost cellulase production, insufficient performance fermentation strains have restricted industrialization biorefinery. Consolidated bioprocessing (CBP) technology combines enzyme in a single bioreactor using specific microorganism or consortium microbes represents another approach worth exploring chemicals from biomass. present review summarizes progress made research CBP conversion. In this review, different strategies lignocellulose are reviewed, including with natural lignocellulose-degrading microorganisms as chassis, biosynthetic microbial co-culturing systems. This provides new perspectives insights on utilization low-cost feedstock biochemicals.

Language: Английский

Citations

24
Composition of Lignocellulose Hydrolysate in Different Biorefinery Strategies: Nutrients and Inhibitors DOI Creative Commons
Yilan Wang, Yuedong Zhang, Qiu Cui

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(10), P. 2275 - 2275

Published: May 11, 2024

The hydrolysis and biotransformation of lignocellulose, i.e., biorefinery, can provide human beings with biofuels, bio-based chemicals, materials, is an important technology to solve the fossil energy crisis promote global sustainable development. Biorefinery involves steps such as pretreatment, saccharification, fermentation, researchers have developed a variety biorefinery strategies optimize process reduce costs in recent years. Lignocellulosic hydrolysates are platforms that connect saccharification downstream fermentation. hydrolysate composition closely related biomass raw pretreatment process, choice biorefining strategies, provides not only nutrients but also possible inhibitors for In this review, we summarized effects each stage lignocellulosic on inhibitors, analyzed huge differences nutrient retention inhibitor generation among various emphasized all lignocellulose need be considered comprehensively achieve maximum optimal control at low cost, reference development chemicals.

Language: Английский

Citations

22
Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production DOI Creative Commons
Maša Vodovnik,

Nataša Lindič

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 79, P. 108523 - 108523

Published: Jan. 31, 2025

Language: Английский

Citations

2
Integrated biorefinery approaches for the industrialization of cellulosic ethanol fuel DOI
Jie Qiao, Haiyang Cui, Minghui Wang

et al.

Bioresource Technology, Journal Year: 2022, Volume and Issue: 360, P. 127516 - 127516

Published: June 25, 2022

Language: Английский

Citations

53
Biosynthesis of artificial starch and microbial protein from agricultural residue DOI Creative Commons
Xinxin Xu, Wei Zhang, Chun You

et al.

Science Bulletin, Journal Year: 2023, Volume and Issue: 68(2), P. 214 - 223

Published: Jan. 1, 2023

Growing populations and climate change pose great challenges to food security. Humankind is confronting a serious question: how will we feed the world in near future? This study presents an out-of-the-box solution involving highly efficient biosynthesis of artificial starch microbial proteins from available abundant agricultural residue as new sources. A one-pot biotransformation using vitro coenzyme-free synthetic enzymatic pathway baker's yeast can simultaneously convert dilute sulfuric acid-pretreated corn stover protein under aerobic conditions. The β-glucosidase-free commercial cellulase mixture plus ex vivo two-enzyme complex containing cellobiose phosphorylase potato α-glucan displayed on surface Saccharomyces cerevisiae, showed better cellulose hydrolysis rates than β-glucosidase-rich mixture. because channeling hydrolytic product solid cellulosic feedstock mitigated inhibition cocktail. Animal tests have shown that digestion amylose results slow relatively small changes blood sugar levels, suggesting it could be health component prevents obesity diabetes. combination utilization non-food biomass address looming crisis food-energy-water nexus.

Language: Английский

Citations

26