Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(34), P. 19071 - 19080
Published: Aug. 14, 2024
Zealexin A1 is a nonvolatile sesquiterpene phytoalexin, which not only exhibits extensive antifungal and insecticidal activities but also has the ability to enhance drought resistance of plants, thus potential applications in agricultural food fields. In this study, biosynthetic pathway zealexin was constructed
Language: Английский
Green Chemistry, Journal Year: 2023, Volume and Issue: 25(5), P. 1704 - 1728
Published: Jan. 1, 2023
Publication of the E Factor drew attention to problem waste in chemicals manufacture. Thirty yeas later it is abundantly clear that underlying cause global environmental problems, from climate change plastic pollution.
Language: Английский
Citations
148
Reviews in Environmental Science and Bio/Technology, Journal Year: 2024, Volume and Issue: 23(2), P. 351 - 383
Published: May 14, 2024
Abstract Polyethylene terephthalate (PET) is one of the most marketed aromatic polyesters in world with an annual demand 2022 approximately 29 million metric tons, expected to increase by 40% 2030. The escalating volume PET waste and current inadequacy recycling methods have led accumulation terrestrial ecosystem, thereby posing significant global health risks. pressing energy environmental issues associated underscore urgent need for “upcycling” technologies. These technologies aim transform reclaimed into higher-value products, addressing both concerns sustainability. Enzyme-mediated biocatalytic depolymerization has emerged as a potentially bio-sustainable method treating plastics. Numerous plastic-degrading enzymes been identified from microbial origins, advancements protein engineering employed modify enhance these enzymes. Microbial metabolic allows development modified chassis capable degrading substrates converting their derived monomers industrial relevant products. In this review, we describe several approaches aiming at enhancing performances PET-degrading present strategies adopted bio-upcycle high-value molecules.
Language: Английский
Citations
12
Methods in enzymology on CD-ROM/Methods in enzymology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
1
ACS Catalysis, Journal Year: 2024, Volume and Issue: 14(7), P. 5469 - 5480
Published: March 28, 2024
Enzymes have selectivity, require mild catalytic conditions, and are important cornerstones in many industrial processes. Protein self-cyclization has opened up the possibility of preserving fragile enzymes during long-term high-temperature catalysis. However, mechanism for improvement thermal tolerance not been elucidated, severely limiting their applications. Herein, we provide a strategy rational design fusion proteins based on structural analysis to obtain cyclized with improved properties. First, constructed that preferentially translated SpyCatcher (CBT) or SpyTag (TBC), both which could form stable single significantly tolerance. Especially, half-life TBC obtained by modifying N-terminal at 40 °C was 10.83 times wild enzymes. Structural revealed terminus protein, SpyCatcher, folded into conformation adversely affected stability. In addition, structure pocket orientation residues CBT were different from those wild-type enzymes, led decrease activity. These conclusions confirmed when another enzyme, sucrose phosphorylase, cyclized. Finally, glucosidase also superior strain preparation ginsenoside F1 high titers as whole-cell catalyst extended use. conclusion, demonstrated first time conjugated long oligopeptide activity stability It necessary translate units less steric hindrance reduce impact protein structure. The provides simple effective modification poor tolerance, providing considerable prospects biosynthesis vivo vitro.
Language: Английский
Citations
8
ChemBioEng Reviews, Journal Year: 2024, Volume and Issue: 11(3), P. 613 - 635
Published: April 29, 2024
Abstract Lignocellulosic biomass, such as plant residues and agricultural waste, holds immense potential a renewable resource for the production of biofuels, chemicals, animal feed. However, efficient degradation lignocellulose into fermentable sugars remains significant challenge. Recent research has highlighted critical role microbial diversity in lignocellulosic biomass degradation, offering new insights from biotechnological perspective. The comprehension utilization are crucial developing strategies degradation. By uncovering intricate relationships between communities their enzymatic machinery, researchers can optimize processes, enhance biofuel production, contribute to more sustainable bio‐based economy. Microorganisms, including bacteria, fungi, archaea, possess diverse capabilities, allowing them secrete plethora lignocellulolytic enzymes. Microbial organisms inhabiting extreme environments, rumen, hot cold springs, deep sea trenches, acidic alkaline pH exhibit generating enzymes, hemicellulolytic which superior biochemical properties essential industrial bioconversion applications. This review explores ability enzymes sources efficiently break down applications biotechnology.
Language: Английский
Citations
5
Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 16
Published: Sept. 12, 2024
Geraniol, an acyclic monoterpene alcohol, has significant potential applications in various fields, including: food, cosmetics, biofuels, and pharmaceuticals. However, the current sources of geraniol mainly include plant tissue extraction or chemical synthesis, which are unsustainable suffer severely from high energy consumption severe environmental problems. The process microbial production recently undergone vigorous development. Particularly, sustainable construction recombinant
Language: Английский
Citations
5
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130545 - 130545
Published: Feb. 29, 2024
Language: Английский
Citations
4
Biochimie, Journal Year: 2024, Volume and Issue: 225, P. 49 - 67
Published: May 9, 2024
The global β-glucosidase market is currently estimated at ∼400 million USD, and it expected to double in the next six years; a trend that mainly ascribed demand for enzyme biofuel processing. Microbial β-glucosidase, particularly, has thus garnered significant attention due its ease of production, catalytic efficiency, versatility, which have all facilitated biotechnological potential across different industries. Hence, there are continued efforts screen, produce, purify, characterize evaluate industrial applicability from actinomycetes, bacteria, fungi, yeasts. With this rising various cost-effective efficient approaches being explored discover, redesign, enhance their production functional properties. Thus, present review provides an up-to-date overview advancements utilization microbial β-glucosidases as "Emerging Green Tools" 21st-century In regard, focus was placed on use recombinant technology, protein engineering, immobilization techniques targeted improving enzyme. Furthermore, insights were given into recent progress made conventional applications, well current commercial status-with patents.
Language: Английский
Citations
4
Process Biochemistry, Journal Year: 2024, Volume and Issue: 145, P. 1 - 12
Published: June 17, 2024
Language: Английский
Citations
4
Bioengineering, Journal Year: 2025, Volume and Issue: 12(1), P. 68 - 68
Published: Jan. 14, 2025
Enzymatic depolymerisation is increasingly recognised as a reliable and environmentally friendly method. The development of this technology hinges on the availability high-quality enzymes associated bioreaction systems for upscaling biodegradation. Microbial heterologous expression have been studied meeting demand. Among these systems, Pichia pastoris system has emerged widely used platform producing secreted proteins. This article provides an overview studies involving recombinant polymer-degrading using P. system. Research interested with depolymerising ability, including cutinase, lipase, laccase, are highlighted in review. key factors influencing discussed, shedding light challenges opportunities biocatalysts through
Language: Английский
Citations
0