Dietary Fishmeal Replacement by Clostridium Autoethanogenum Protein Meal Influences the Nutritional and Sensory Quality of Turbot (Scophthalmus Maximus) Via the TOR/AAR/AMPK Pathways DOI
Zezheng Qi, Nan Bai, Qing Li

et al.

Published: Jan. 1, 2023

Clostridium autoethanogenum protein (CAP) is a promising source for aquaculture; however, how CAP influences fish quality worth extensive research. We randomly allocated 630 turbot with initial body weights of about 180 g into six groups, fishmeal-based control diet or to replace 15% (CAP15), 30%, 45%, 60%, 75% fishmeal protein. After 70-day feeding trial, the fillet yield and contents protein, collagen, hydroxyproline, C20:5n-3, ∑n-3/∑n-6 polyunsaturated fatty acids ratio in muscle linearly decreased increase. However, fed CAP15 maintained these parameters. By contrast, hardness was increased by CAP, accompanied reduced fiber volume expression myogenesis genes, including ctsd, ctsl, murfl. Phosphorylation Akt, TOR, 4EBP-1, S6 reduced; phosphorylation AMPK, eIF2α, abundance ATF4 lineally suggesting that TOR signaling pathway inhibited, AAR AMPK pathways were activated. Meantime, gene related degradation, myf5, myod, pax7, downregulated CAP. In conclusion, replaced up without negatively impacting quality. higher levels yield, content, diameter but hardness, which could be attributed inhibition CAP's activation pathways.

Language: Английский

Sprayed Oil–Water Microdroplets as a Hydrogen Source DOI

Xuke Chen,

Yu Xia,

Yingfeng Wu

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(15), P. 10868 - 10874

Published: April 4, 2024

Liquid water provides the largest hydrogen reservoir on earth's surface. Direct utilization of as a source atoms and molecules is fundamental to evolution ecosystem industry. However, liquid an unfavorable electron donor for forming these species owing its redox inertness. We report oil-mediated extraction from microdroplets, which easily achieved by ultrasonically spraying oil–water emulsion. Based charge measurement paramagnetic resonance spectroscopy, contact electrification between oil microdroplet demonstrated be origin molecules. This results in enhanced separation subsequent mutual neutralization, enables ∼13-fold increase carriers comparison with ultrapure spray, leading ∼16-fold spray-sourced that can hydrogenate CO2 selectively produce CO. These findings emphasize potential enabled emulsion hydrophobic driving hydrogenation reactions.

Language: Английский

Citations

11
Converting CO2 to single-cell protein via an integrated electrocatalytic-biosynthetic system DOI
Huijuan Cui, Weisong Liu, Chunling Ma

et al.

Applied Catalysis B Environment and Energy, Journal Year: 2024, Volume and Issue: 350, P. 123946 - 123946

Published: April 24, 2024

Language: Английский

Citations

11
Coupling Waste Feedstocks to Microbial Protein Production in a Circular Food System DOI
Taylor Uekert, Alissa Bleem, Eric C. Holmes

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Global food production is a major contributor to greenhouse gas emissions, water consumption, and land use. As an alternative conventional agriculture, the of waste-derived microbial protein (MP) holds promise for reducing environmental impacts. MP can be mass-produced in volumetrically scalable fermentation processes on short time scales, enabling facile scale-up with lower use, impacts than animal and, some cases, plant protein. also produced from waste feedstocks, diverting landfills or natural environment. This Perspective explores availability suitability feedstocks production, suggesting that generated United States could fulfill twice current national demand. We discuss biotechnological separations required produce food-grade human consumption waste. Key challenges include consistency, consumer regulatory acceptance, process utilities (electricity, heat, nutrients) account up 85% costs most impacts, all which present opportunities innovation microbiology design spaces. Overall, this work highlights potential contribute more circular, resilient, sustainable system.

Language: Английский

Citations

2
Bacterial single cell protein (BSCP): A sustainable protein source from methylobacterium species DOI Creative Commons
Marttin Paulraj Gundupalli, Sara Ansari, Jaquelinne Pires Vital da Costa

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 147, P. 104426 - 104426

Published: March 23, 2024

Single Cell Protein (SCP) is a potential replacement for traditional protein sources in both food and feed. This review specifically focuses on SCP produced by bacteria, emphasizing its unique advantages such as rapid growth, high content, efficient manufacturing, genetic flexibility, thorough strain characterization. Notably, among other microorganisms, Methylobacterium species have recently been found to be crucial synthesis. The examines the feasibility of incorporating sustainable substitute diets aquaculture cattle production closely analyzing fishmeal soybean meal. Additionally, it highlights importance ongoing research supportive legislation, while also considering economic policy factors assess financial viability production. concludes outlining future prospects outlook derived from species, providing forward-looking perspective evolving applications SCP.

Language: Английский

Citations

9
Ruminations on sustainable and safe food: Championing for open symbiotic cultures ensuring resource efficiency, eco‐sustainability and affordability DOI Creative Commons
U. Javourez, Silvio Matassa, Siegfried E. Vlaeminck

et al.

Microbial Biotechnology, Journal Year: 2024, Volume and Issue: 17(3)

Published: March 1, 2024

Abstract Microbes are powerful upgraders, able to convert simple substrates nutritional metabolites at rates and yields surpassing those of higher organisms by a factor 2 10. A summary table highlights the superior efficiencies whole array microbes compared conventionally farmed animals insects, converting nitrogen organics food feed. Aiming most resource‐efficient class microbial proteins, deploying power open communities, coined here as ‘symbiotic microbiomes’ is promising. For instance, production train interest develop rumen‐inspired technologies upgrade fibre‐rich substrates, increasingly available residues from emerging bioeconomy initiatives. Such advancements offer promising perspectives, currently only 5%–25% cellulose recovered ruminant livestock systems. While safely producing feed with cultures has long‐standing tradition, novel symbiotic fermentation routes facing much market entrance barriers axenic fermentation. Our global society pivotal juncture, requiring shift towards systems that not embrace environmental economic sustainability but also uphold ethical standards. In this context, we propose re‐examine place spontaneous or natural consortia for safe future biotech developments, advocate intelligent regulatory practices. We stress reconsidering microbiomes key achieve sustainable development goals defend need biotechnology literacy education.

Language: Английский

Citations

7
Current trends and possibilities of typical microbial protein production approaches: a review DOI

JinTao He,

Min Tang,

FeiFei Zhong

et al.

Critical Reviews in Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 18

Published: April 2, 2024

Global population growth and demographic restructuring are driving the food agriculture sectors to provide greater quantities varieties of food, which protein resources particularly important. Traditional animal-source proteins becoming increasingly difficult meet demand current consumer market, search for alternative sources is urgent. Microbial biomass obtained from nonpathogenic single-celled organisms, such as bacteria, fungi, microalgae. They contain large amounts essential amino acids well a variety other nutritive substances, considered be promising sustainable alternatives traditional proteins. In this review, typical approaches microbial synthesis processes were highlighted characteristics applications different types described. Bacteria, microalgae can individually or co-cultured obtain protein-rich using starch-based raw materials, organic wastes, one-carbon compounds fermentation substrates. have been gradually used in practical foods, nutritional supplements, flavor modifiers, animal feeds. However, further development application require more advanced biotechnological support, screening good strains, safety considerations. This review contributes accelerating resource provides solution crisis facing world.

Language: Английский

Citations

5
Utilizing Clostridium autoethanogenum for dietary protein in aquafeeds: Current progress in research and future perspectives DOI Creative Commons
Sahya Maulu

Journal of Applied Aquaculture, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 19

Published: April 13, 2024

The aquaculture sector faces sustainability challenges due to unsustainable feedstuffs. To address this, there's a continuous search for alternatives traditional aquafeed protein sources such as fishmeal. Clostridium autoethanogenum is novel source with long-standing application in the bio-energy industry. With 80–89% crude content on dry matter basis, (CAP) has gained attention nutrition research species. This study aimed review CAP utilization aquafeed, examining its nutritional value and impacts aquatic animals' growth, feed efficiency, digestion, absorption, immune response. Research suggests promising potential showing achieving complete fishmeal replacement without compromising growth performance response select Nonetheless, higher inclusion levels show adverse effects health status of certain fish species, indicating variability among species utilize CAP-based diets. Further investigations are required understand this outcomes reported

Language: Английский

Citations

5
The enhancement of energy supply in syngas-fermenting microorganisms DOI
Yida Zhai, Sheng Tong,

Limei Chen

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 252, P. 118813 - 118813

Published: April 2, 2024

Language: Английский

Citations

4
Assessing the Mass Transfer Coefficient in Jet Bioreactors with Classical Computer Vision Methods and Neural Networks Algorithms DOI Creative Commons
Irina Nizovtseva, Vladimir Palmin, Ivan V. Simkin

et al.

Algorithms, Journal Year: 2023, Volume and Issue: 16(3), P. 125 - 125

Published: Feb. 21, 2023

Development of energy-efficient and high-performance bioreactors requires progress in methods for assessing the key parameters biosynthesis process. With a wide variety approaches determining phase contact area gas–liquid flows, question obtaining its accurate quantitative estimation remains open. Particularly challenging are issues getting information about mass transfer coefficients instantly, as well development predictive capabilities implementation effective flow control continuous fermentation both on laboratory industrial scales. Motivated by opportunity to explore possibility applying classical non-classical computer vision results high-precision video records bubble flows obtained during experiment bioreactor vessel, we number presented paper. Characteristics bioreactor’s were estimated first (CCV) including an elliptic regression approach single boundaries selection clustering, image transformation through set filters developing algorithm separation overlapping bubbles. The application developed method entire filming makes it possible obtain parameter distributions dropout thresholds order better estimates due averaging. CCV methodology was also tested verified collected labeled manual dataset. An onwards deep neural network (NN) applied, instance segmentation task, has demonstrated certain advantages terms high resolution, while one tends be more speedy. Thus, current manuscript disadvantages discussed based evaluation bubbles’ their defined. coefficient virtue is represented.

Language: Английский

Citations

10
Dietary fishmeal replacement by Clostridium autoethanogenum protein meal influences the nutritional and sensory quality of turbot (Scophthalmus maximus) via the TOR/AAR/AMPK pathways DOI Creative Commons
Zezheng Qi, Nan Bai, Qing Li

et al.

Animal nutrition, Journal Year: 2024, Volume and Issue: 18, P. 84 - 95

Published: May 6, 2024

protein (CAP) is a promising source for aquaculture; however, how CAP influences fish quality worth extensive research. We randomly allocated 630 turbot with initial body weights of about 180 g into 6 groups, fishmeal-based control diet or replacing 15% (CAP15), 30% (CAP30), 45% (CAP45), 60% (CAP60), 75% (CAP75) fishmeal protein. After 70-d feeding trial, the fillet yield (

Language: Английский

Citations

4