Stabilization challenges and aggregation in protein-based therapeutics in the pharmaceutical industry

RSC Advances, Journal Year: 2023, Volume and Issue: 13(51), P. 35947 - 35963

Published: Jan. 1, 2023

Protein-based therapeutics have revolutionized the pharmaceutical industry and become vital components in development of future therapeutics. They offer several advantages over traditional small molecule drugs, including high affinity, potency specificity, while demonstrating low toxicity minimal adverse effects. However, manufacturing processes protein-based presents challenges related to protein folding, purification, stability immunogenicity that should be addressed. These proteins, like other biological molecules, are prone chemical physical instabilities. The drugs throughout entire manufacturing, storage delivery process is essential. occurrence structural instability resulting from misfolding, unfolding, modifications, as well aggregation, poses a significant risk efficacy these overshadowing their promising attributes. Gaining insight into alterations caused by aggregation impact on for advancement refinement Hence, this review, we discussed some features during production, formulation stabilization strategies engineering computational methods prevent aggregation.

Language: Английский

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Immunomodulatory Compounds from the Sea: From the Origins to a Modern Marine Pharmacopoeia

Marine Drugs, Journal Year: 2024, Volume and Issue: 22(7), P. 304 - 304

Published: June 28, 2024

From sea shores to the abysses of deep ocean, marine ecosystems have provided humanity with valuable medicinal resources. The use organisms is discussed in ancient pharmacopoeias different times and geographic regions still deeply rooted traditional medicine. Thanks present-day, large-scale bioprospecting rigorous screening for bioactive metabolites, ocean coming back as an untapped resource natural compounds therapeutic potential. This renewed interest drugs propelled by a burgeoning research field investigating molecular mechanisms which newly identified intervene pathophysiology human diseases. Of great clinical relevance are molecules endowed anti-inflammatory immunomodulatory properties emerging applications management chronic inflammatory disorders, autoimmune diseases, cancer. Here, we review historical development pharmacology Eastern Western worlds describe status drug discovery. Finally, discuss importance conducting sustainable exploitation resources through biotechnology.

Language: Английский

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Escherichia coli in the production of biopharmaceuticals

Biotechnology and Applied Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 8, 2024

Abstract Escherichia coli has shouldered a massive workload with the discovery of recombinant DNA technology. A new era began in biopharmaceutical industry production insulin, first protein, E. and its use treating diabetes. After many biopharmaceuticals produced from have been approved by US Food Drug Administration European Medicines Agency to treat various human diseases. Although some disadvantages, such as lack post‐translational modifications toxicity, it is an important host advantages being well‐known bacterium protein production, cheap, simple system, high yield. This study examined under headings peptides, hormones, enzymes, fusion proteins, antibody fragments, vaccines, other pharmaceuticals. The topics on which these were for diseases, when company they produced, their development field are included.

Language: Английский

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Advances in bacterial glycoprotein engineering: A critical review of current technologies, emerging challenges, and future directions

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108514 - 108514

Published: Jan. 1, 2025

Language: Английский

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Advances and Challenges in Biomanufacturing of Glycosylation of Natural Products

Fermentation, Journal Year: 2024, Volume and Issue: 10(7), P. 349 - 349

Published: July 9, 2024

Glycosylation is one of the most common and important modifications in natural products (NPs), which can alter biological activities properties NPs, effectively increase structural diversity, improve pharmacological activities. The biosynthesis glycosylation involves multiple complex processes, are coordinated by many enzymes. UDP-glycosyltransferases (UGTs) play a crucial role modification, have attracted long-term widespread research attention. UGTs catalyze O-, C-, S-, N-glycosylation different substrates, producing variety glycosides with broad activity, while improving solubility, stability, bioavailability, other functions NPs. In recent years, rapid development synthetic biology advanced manufacturing technologies, especially application artificial intelligence field biology, has led to series new discoveries NP UGT. This work summarizes latest progress challenges glycosylation, covering results potential applications glycosylated derivatives terpenes, flavonoids, polyphenols, aromatic compounds, compounds terms biogenesis. Looking future, may leverage intelligence-driven techniques decipher genes related pathway, expected further promote large-scale synthesis diversity NPs pharmaceutical, functional food, cosmetic industries.

Language: Английский

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Microalgae in health care and functional foods: β-glucan applications, innovations in drug delivery and synthetic biology

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 4, 2025

Microalgae are emerging as a key player in healthcare, functional foods, and sustainable biotech due to their capacity produce bioactive compounds like β-glucans, omega-3 fatty acids, antioxidants an eco-friendly manner. This review comprehensively discusses the role of microalgae healthcare focusing particularly on β-glucan therapeutics, drug delivery innovations, synthetic biology applications. In microalgae-derived show immense promise for treating diseases, boosting immunity, tackling oxidative stress. Euglena -derived paramylon, type β-glucan, has shown potential various medical applications, including immunomodulation anticancer therapy. Synthetic bioprocess engineering enhancing microalgae’s therapeutic nutritional value, with applications personalized medicine. To maximize microalgae, further research development needed address scalability, regulatory alignment, consumer acceptance, focus interdisciplinary collaboration practices align innovation environmental conservation.

Language: Английский

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Stepwise activation of gene copies results in higher final titers of subclones compared to immediate integration of the full set of active copies

New Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Unlocking the power of antimicrobial peptides: advances in production, optimization, and therapeutics

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 28, 2025

Antimicrobial peptides (AMPs) are critical effectors of innate immunity, presenting a compelling alternative to conventional antibiotics amidst escalating antimicrobial resistance. Their broad-spectrum efficacy and inherent low resistance development countered by production challenges, including limited yields proteolytic degradation, which restrict their clinical translation. While chemical synthesis offers precise structural control, it is often prohibitively expensive complex for large-scale production. Heterologous expression systems provide scalable, cost-effective platform, but necessitate optimization. This review comprehensively examines established emerging AMP strategies, encompassing fusion protein technologies, molecular engineering approaches, rational peptide design, post-translational modifications, with an emphasis on maximizing yield, bioactivity, stability, safety. Furthermore, we underscore the transformative role artificial intelligence, particularly machine learning algorithms, in accelerating discovery optimization, thereby propelling expanded therapeutic application contributing global fight against drug-resistant infections.

Language: Английский

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Tobacco as green bioreactor for therapeutic protein production: latest breakthroughs and optimization strategies

Plant Growth Regulation, Journal Year: 2023, Volume and Issue: 103(2), P. 227 - 241

Published: Dec. 10, 2023

Language: Английский

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Phototactic/Photosynthetic/Magnetic‐Powered Chlamydomonas Reinhardtii‐Metal‐Organic Frameworks Micro/Nanomotors for Intelligent Thrombolytic Management and Ischemia Alleviation

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(29)

Published: Aug. 18, 2024

Thrombosis presents a critical health threat globally, with high mortality and incidence rates. Clinical treatment faces challenges such as low thrombolytic agent bioavailability, thrombosis recurrence, ischemic hypoxia damage, neural degeneration. This study developed biocompatible Chlamydomonas Reinhardtii micromotors (CHL) photo/magnetic capabilities to address these needs. These CHL micromotors, equipped phototaxis photosynthesis abilities, offer promising solutions. A core aspect of this innovation involves incorporating polysaccharides (glycol chitosan (GCS) fucoidan (F)) into ferric Metal-organic frameworks (MOFs), loaded urokinase (UK), subsequently self-assembled onto the multimodal CHL, forming core-shell microstructure (CHL@GCS/F-UK-MOF). Under light-navigation, CHL@GCS/F-UK-MOF is shown penetrate thrombi, enhancing biodistribution. Combining magnetic hyperthermia technique achieves stimuli-responsive multiple-release, accelerating thrombolysis rapidly restoring blocked blood vessels. Moreover, approach attenuates thrombi-induced disorder tissue damage. The photosynthetic magnetotherapeutic properties CHL@GCS/F-UK-MOF, along their protective effects, including reduced apoptosis, enhanced behavioral function, induced Heat Shock Protein (HSP), polarized M2 macrophages, mitigated hypoxia, are confirmed through biochemical, microscopic, assessments. multifunctional biomimetic platform, integrating photo-magnetic techniques, offers comprehensive cardiovascular management, advancing related technologies.

Language: Английский

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