TUBA1B as a novel prognostic biomarker correlated with immunosuppressive tumor microenvironment and immunotherapy response DOI Creative Commons

Juntao Qi,

Mingming Zhou, Na Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 4, 2025

Background: Tubulin alpha 1b (TUBA1B) is a key microtubule protein essential for maintaining cellular structure and function. This contributes significantly to cytoskeletal formation implicated in various diseases. Despite its fundamental roles, TUBA1B’s impact on tumor prognosis the immune microenvironment across cancer types remains inadequately understood. Methods To elucidate role response, we conducted comprehensive analysis, integrating data from established databases such as The Cancer Genome Atlas, Genotype Tissue Expression, Cell Lineage Encyclopedia, Human Protein Kaplan-Meier Plotter, cBioPortal, TIMER, ImmuCellAI, along with large-scale clinical study immunotherapy cohort. We also vitro functional assays assess cells, allowing detailed examination of relationship modulation. Results: Our findings indicate that TUBA1B expression dysregulated multiple cancers, correlating strongly poor survival outcomes advanced pathological stages. Functional enrichment analyses further revealed regulates cell cycle processes, driving proliferation, migration, invasion. It influences functions within both innate adaptive systems, affecting immune-related signaling pathways. These insights underscore multifaceted progression response. Conclusion: highlights potential human oncogene substantial roles tumorigenesis regulation. Elevated levels are associated an immunosuppressive microenvironment, impacting treatment outcomes. Targeting may offer promising therapeutic avenues enhancing treatment, offering new perspectives innovative anti-tumor strategies high impact.

Language: Английский

Identification of the novel exhausted T cell CD8 + markers in breast cancer DOI Creative Commons
Hengrui Liu,

Angela Dong,

Ayana Meegol Rasteh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 19, 2024

Cancer is one of the most concerning public health issues and breast cancer common cancers in world. The immune cells within tumor microenvironment regulate development. In this study, single cell data sets were used to identify marker gene for exhausted CD8 + T (CD8Tex) cancer. Machine learning methods cluster subtypes establish prognostic models with bulk using evaluate impacts CD8Tex. We analyzed overexpressing survival-associated genes identified CD8Tex hub protein-protein-interaction network. relevance T-cells was evaluated. clinical associations sequencing spatial data. pan-cancer expression, survival, association analyzed. biomarker CD8Tex-based subtyping systems performed well separation patients different survival. CRTAM, CLEC2D, KLRB1 as demonstrated have potential therapy impact. This study provides a unique view critical therapy.

Language: Английский

Citations

42
Genetic expression in cancer research: Challenges and complexity DOI
Hengrui Liu, Zheng Guo, Panpan Wang

et al.

Gene Reports, Journal Year: 2024, Volume and Issue: unknown, P. 102042 - 102042

Published: Sept. 1, 2024

Language: Английский

Citations

29
Is the voltage-gated sodium channel β3 subunit (SCN3B) a biomarker for glioma? DOI
Hengrui Liu, Jieling Weng, Christopher Huang

et al.

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(5)

Published: Sept. 18, 2024

Language: Английский

Citations

18
Single-cell and bulk RNA sequencing analysis reveals CENPA as a potential biomarker and therapeutic target in cancers DOI Creative Commons
Hengrui Liu,

Miray Karsidag,

Kunwer Chhatwal

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(1), P. e0314745 - e0314745

Published: Jan. 16, 2025

Cancer remains one of the most significant public health challenges worldwide. A widely recognized hallmark cancer is ability to sustain proliferative signaling, which closely tied various cell cycle processes. Centromere Protein (CENPA), a variant standard histone H3, crucial for selective chromosome segregation during cycle. Despite its importance, comprehensive pan-cancer bioinformatic analysis CENPA has not yet been conducted. Data on genomes, transcriptomes, and clinical information were retrieved from publicly accessible databases. We analyzed CENPA's genetic alterations, mRNA expression, functional enrichment, association with stemness, mutations, expression across populations cellular locations, link cycle, impact survival, relationship immune microenvironment. Additionally, prognostic model glioma patients was developed demonstrate potential as biomarker. Furthermore, drugs targeting in cells identified predicted using drug sensitivity correlations protein-ligand docking. exhibited low levels gene mutation cancers. It found be overexpressed nearly all types TCGA, relative normal controls, predominantly located nucleus malignant cells. showed strong particularly biomarker G2 phase. also emerged valuable diagnostic multiple types. In glioma, demonstrated reliable when used alongside other factors. linked Drugs such CD-437, 3-Cl-AHPC, Trametinib, BI-2536, GSK461364 target serves cancers, offering both value.

Language: Английский

Citations

4
Nanozyme-based synergistic therapeutic strategies against tumors DOI
Ye Zhang, Ning Zhang, Shouping Gong

et al.

Drug Discovery Today, Journal Year: 2025, Volume and Issue: unknown, P. 104292 - 104292

Published: Jan. 1, 2025

Language: Английский

Citations

1
Rational Identification of Ritonavir as IL-20 Receptor A Ligand Endowed with Antiproliferative Properties in Breast Cancer Cells DOI Open Access

Valentina Maggisano,

Adriana Gargano, Jessica Maiuolo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(3), P. 1285 - 1285

Published: Feb. 2, 2025

Targeting the tumor microenvironment (TME) is an attractive strategy for developing new drugs with anticancer activity against triple-negative breast cancer (TNBC). Interleukins (ILs) are key players in TME cytokine network promoting progression. Recent studies have highlighted involvement of IL-20 receptor subunit alpha (IL-20RA) signalling several cancers, including BC, which IL-20RA highly expressed, correlating poor prognosis and influencing tumoral characteristics such as proliferation, cell death, invasiveness, activity. Therefore, elucidating role pathway could form basis therapeutic strategies. This study aimed to identify selective bioactive ligands able affect Virtual screening over 310,000 compounds from both DrugBank ZINC15 databases identified four potential hit tested their TNBC vitro lines. Notably, Ritonavir, a well-known Human Immunodeficiency Virus Type 1 (HIV-1) protease inhibitor, significantly inhibited proliferation (about 40% at 50 µM, p < 0.001). preincubation counteracted Ritonavir’s cytostatic effect while knockdown restored Ritonavir-treated cells. In conclusion, these findings demonstrated that Ritonavir reduced through modulation, suggesting its repurposing agent management.

Language: Английский

Citations

1
ADME gene-driven prognostic model for bladder cancer: a breakthrough in predicting survival and personalized treatment DOI Creative Commons
Haojie Dai, Xi Zhang, You‐Yang Zhao

et al.

Hereditas, Journal Year: 2025, Volume and Issue: 162(1)

Published: March 19, 2025

Abstract Background Genes that participate in the absorption, distribution, metabolism, excretion (ADME) processes occupy a central role pharmacokinetics. Meanwhile, variability clinical outcomes and responses to treatment is notable bladder cancer (BLCA). Methods Our study utilized expansive datasets from TCGA GEO explore prognostic factors cancer. Utilizing both univariate Cox regression lasso techniques, we identified ADME genes critical for patient outcomes. our study, model assessing risk was constructed. The evaluation of this model's predictive precision conducted using Kaplan–Meier survival curves assessments based on ROC curves. Furthermore, devised nomogram, offering straightforward visualization crucial indicators. To potential mediating differences between high low groups, performed comprehensive analyses including Gene Ontology (GO) Kyoto Encyclopedia Genomes (KEGG)-based enrichment analyses, immune infiltration variations, somatic mutation landscapes, pharmacological sensitivity response assessment etc. Immediately following this, selected core PPI network explored as well modulation, pathway activation. And differential expression verified by immunohistochemistry qRT-PCR. Finally pan-cancer biomarkers. Results efforts culminated establishment validated 17-gene ADME-centered prediction model, displaying remarkable accuracy BLCA prognosis. Through separate cox importance model’s score forecasting substantiated. novel nomogram incorporating variables alongside introduced. Comprehensive studies established strong correlation several key indicators: patterns cell infiltration, reactions immunotherapy, landscape profiles drug sensitivity. We screened gene CYP2C8, its tumor bioregulation upregulated found it can serve reliable biomarker pan-cancer. Conclusion formulated research stands formidable instrument prognosis, while also providing insights into disease's progression mechanisms guiding decision-making strategies.

Language: Английский

Citations

1
Unraveling the role of withanolides as key modulators in breast cancer mitigation DOI
Huma Hameed,

Maham Afzal,

Mahtab Ahmad Khan

et al.

Molecular Biology Reports, Journal Year: 2025, Volume and Issue: 52(1)

Published: March 21, 2025

Language: Английский

Citations

1
Artemisinin and Its Derivatives as Potential Anticancer Agents DOI Creative Commons

L Y Wen,

Ben Chung-Lap Chan, Ming‐Hua Qiu

et al.

Molecules, Journal Year: 2024, Volume and Issue: 29(16), P. 3886 - 3886

Published: Aug. 16, 2024

Artemisinin is a natural sesquiterpene lactone obtained from the traditional Chinese medicinal herb Artemisia annua L. (qinghao). and its derivatives share an unusual endoperoxide bridge are extensively used for malaria treatment worldwide. In addition to antimalarial activities, artemisinin have been reported exhibit promising anticancer effects in recent decades. this review, we focused on research progress of with potential activities. The pharmacological effects, mechanisms, clinical trials cancer therapy were discussed. This review may facilitate future exploration as effective agents.

Language: Английский

Citations

7
Potential role of P4HB in the tumor microenvironment and its clinical prognostic value: a comprehensive pan-cancer analysis and experimental validation with a focus on KIRC DOI Creative Commons

L. Zhang,

Yuwei Li, Tianyi Xie

et al.

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 3, 2025

Tumor microenvironment (TME) plays a crucial role in tumor growth and metastasis. Exploring biomarkers that are significantly associated with TME can help guide individualized treatment of patients. We analyzed the expression survival P4HB pan-cancer through TCGA database, verified protein level by HPA database. In addition, we used Metascape database to construct protein–protein interaction networks single-cell Sequencing for functional analysis. An immune cell infiltration analysis was performed explore potential TME. further analyze relationship between checkpoint molecules blockade therapy. Finally, oncogenic RCC cells validated using colony formation wound healing assays. RNA levels were extensively up-regulated pan-cancer. However, high poor KIRC. The clinical relevance analyses suggested advanced TNM stage. Moreover, multivariate cox regression indicated (HR = 1.372, 95% CI 1.047–1.681, P 0.019) an independent risk factor OS Functional revealed is involved hypoxia, system processes. Our study also found correlated elevated CD8 + T M2 macrophages. results assays showed knockdown inhibited migration. specific biomarker KIRC prognosis characteristics. may play influential ICB

Language: Английский

Citations

0