Carbohydrate Polymers,
Journal Year:
2023,
Volume and Issue:
324, P. 121554 - 121554
Published: Nov. 3, 2023
Daphne
mezereum
L.,
an
important
medicinal
plant
in
Scandinavian
folk
medicine,
was
used
to
treat
ailments
such
as
diarrhea,
swelling
and
stomach
pain.
A
range
of
natural
compounds
have
been
isolated,
but
little
attention
has
given
the
polysaccharides
this
plant.
Previous
work
our
group
shown
that
a
polysaccharide
enriched
fraction
from
bark
D.
exhibited
pro-inflammatory
effects.
To
pursue
further,
aim
present
isolate
characterize
these
polysaccharides.
From
ethanol-precipitate
water
extract,
one
neutral
(DMP-NF)
acidic
(DMP-AF)
isolated
by
anion-exchange
chromatography.
GC,
GC-MS
1D-
2D-NMR
were
structures.
DMP-NF
appeared
be
mixture
arabinan,
arabinogalactan
hemicelluloses
xyloglucan,
mannan
xylan.
DMP-AF
contained
pectic
mainly
consisting
unusually
long
homogalacturonan
backbone.
Enzymatic
treatment
pectinase
yielded
DMP-ED,
which
rhamnogalacturonan-I
backbone
with
galactan
side
chains.
Both
DMP-ED
induced
IFN-γ
TNF-α
secretion
peripheral
blood
mononuclear
cells
(PBMCs),
being
most
potent
fraction.
less
active,
might
due
sterically
available
domain.
Carbohydrate Polymers,
Journal Year:
2024,
Volume and Issue:
338, P. 122167 - 122167
Published: April 17, 2024
2-Hydroxypropyl-β-cyclodextrin
(HPBCD)
is
one
of
the
most
important
cyclodextrin
derivatives,
finding
extensive
applications
in
pharmaceutical
sector.
Beyond
its
role
as
an
excipient,
HPBCD
achieved
orphan
drug
status
2015
for
Niemann-Pick
type
C
disease
treatment,
prompting
research
into
therapeutic
potential
various
disorders.
However,
acceptance
active
ingredient
may
be
impeded
by
complex
nature.
Indeed,
not
a
single
entity
with
well-defined
structure,
instead,
it
mixture
isomers
varying
substituent
positions
and
degree
hydroxypropylation,
posing
several
challenges
unambiguous
characterization.
Pharmacopoeias'
methods
only
address
average
hydroxypropylation
extent,
lacking
rapid
approach
to
characterize
on
CD
scaffold.
Recognizing
that
distribution
substituents
significantly
influences
complexation
ability
overall
activity
derivative,
primarily
altering
cavity
dimensions,
we
present
straightforward
non-destructive
method
based
liquid
state
NMR
spectroscopy
analyze
hydroxypropyl
sidechains.
This
relies
set
routine
experiments
establish
quantitative
assignment
provides
simple
yet
effective
tool
disclose
substitution
pattern
this
material,
utilizing
easily
accessible
(400
MHz
NMR)
instrumentation.
microLife,
Journal Year:
2024,
Volume and Issue:
5
Published: Jan. 1, 2024
The
mimivirus
1.2
Mb
genome
was
shown
to
be
organized
into
a
nucleocapsid-like
genomic
fiber
encased
in
the
nucleoid
compartment
inside
icosahedral
capsid.
protein
shell
is
composed
of
mixture
two
GMC-oxidoreductase
paralogs,
one
them
being
main
component
glycosylated
layer
fibrils
at
surface
virion.
In
this
study,
we
determined
effect
deletion
each
corresponding
genes
on
and
fibrils.
First,
deleted
GMC-oxidoreductase,
most
abundant
fiber,
its
structure
composition
mutant.
As
expected,
it
second
contained
5-
6-start
helices
similar
wild-type
fiber.
This
result
led
us
propose
model
explaining
their
coexistence.
Then
fibrils,
analyze
Second,
showed
that
fitness
single
mutants
double
mutant
were
not
decreased
compared
with
viruses
under
laboratory
conditions.
Third,
deleting
did
impact
glycosylation
or
glycan
despite
modifying
composition.
Because
machinery
members
different
clades
are
different,
expanded
analysis
B
C
among
three
even
isolates
within
same
clade.
Taken
together,
results
obtained
distinct
central
processes
(genome
packaging
virion
coating)
illustrate
an
unexpected
functional
redundancy
family
Mimiviridae,
suggesting
may
major
evolutionary
force
behind
giant
genomes.
Polymers,
Journal Year:
2022,
Volume and Issue:
14(19), P. 3986 - 3986
Published: Sept. 23, 2022
The
use
of
natural
polysaccharides
as
biomaterials
is
gaining
importance
in
tissue
engineering
due
to
their
inherent
biocompatibility.
In
this
direction,
the
present
study
aims
explore
structure
and
biocompatibility
EPS
produced
by
Virgibacillus
dokdonensis
VITP14.
This
marine
bacterium
produces
17.3
g/L
at
96
h
fermentation.
was
purified
using
ion
exchange
gel
permeation
chromatographic
methods.
porous
web-like
elemental
composition
(C,
O,
Na,
Mg,
P,
S)
were
inferred
from
SEM
EDX
analysis.
AFM
analysis
revealed
spike-like
lumps
with
a
surface
roughness
84.85
nm.
zeta
potential
value
−10
mV
indicates
anionic
nature
EPS.
Initial
molecular
characterization
showed
that
heteropolysaccharide
composed
glucose
(25.8%),
ribose
(18.6%),
fructose
(31.5%),
xylose
(24%),
which
are
monosaccharide
units
HPLC
FTIR
spectrum
presence
functional
groups/bonds
typical
EPSs
(O-H,
C-H,
C-O-H,
C-O,
S=O,
P=O).
polymer
has
an
average
weight
555
kDa.
Further,
NMR
monomer
composition,
existence
two
α-
six
β-glycosidic
linkages,
branched
repeating
unit
→
1)[α-D-Xylp-(1
2)-α-D-Glcp-(1
6)-β-D-Glcp-(1
5)]-β-D-Frup-(2
2)[β-D-Xylp-(1
4)]-β-D-Xylp-(1
6)-β-D-Fruf-(2
4)-β-D-Ribp-(1
→.
thermally
stable
till
251.4
°C.
X-ray
diffraction
confirmed
semicrystalline
(54.2%)
exhibits
significant
water
solubility
(76.5%),
water-holding
capacity
(266.8%),
emulsifying
index
(66.8%),
hemocompatibility
(erythrocyte
protection
>
87%),
cytocompatibility
(cell
viability
80%
on
RAW264.7
keratinocyte
HaCaT
cells)
higher
concentrations
prolongs
coagulation
time
APTT
PT
tests.
Our
research
unveils
VITP14
for
synthesizing
variety
biomaterials.
