Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(11), P. 104217 - 104217
Published: Aug. 14, 2024
Trimethyltin
chloride
(TMT)
is
widespread
in
the
environment
and
harmful
to
both
humans
animals.
In
order
investigate
toxicity
mechanism
of
TMT
exposure
on
chicken
liver,
We
established
an
vivo
experimental
model
by
giving
chickens
oral
administration
different
concentrations
dilution
solution
vitro
experiments
treating
leghorn
male
hepatoma
(LMH)
cells
for
12
h.
The
results
showed
that
Albumin
(ALB),
total
protein
(TP)
alanine
aminotransferase
(ALT)
blood
TMT-treated
chickens,
as
well
ALT
aspartate
(AST)
were
dose-dependently
increased,
degrees
necrosis
hepatocytes
observed
histology.
Meanwhile,
led
a
significant
decrease
glutathione
(GSH)
content
liver
tissues
LMH
cells,
what's
more
increase
malondialdehyde
(MDA)
cell
supernatants.
expression
apoptosis-related
genes
Caspase8,
Caspase3
Caspase9
increased
after
treated
TMT,
percentage
late
apoptosis
cells.
This
suggests
can
cause
oxidative
stress
livers
resulting
injury.
Animals,
Journal Year:
2025,
Volume and Issue:
15(3), P. 387 - 387
Published: Jan. 30, 2025
Tea
polyphenol
(TP)
is
a
kind
of
natural
macromolecular
compound
present
in
tea
extract
with
rich
biological
potential.
The
purpose
this
study
was
to
explore
the
protective
effect
TP
on
intestinal
immune
barrier
and
related
mechanisms
alleviating
injury.
Models
common
carp
(Cyprinus
carpio
L.)
primary
epithelial
cells
treated
lipopolysaccharide
(LPS)
were
established.
qPCR
ELISA
results
showed
that
increased
levels
lysozyme
(LZ),
alkaline
phosphatase
(ALP),
complement
component
3
(C3),
4
(C4),
immunoglobulin
T
(IgT),
D
(IgD),
M
(IgM)
activate
ability.
Molecular
docking
indicated
had
strong
interaction
TLR2.
Meanwhile,
alleviated
LPS-induced
inflammatory
damage
as
evidenced
by
reducing
mRNA
TNF-α,
IL-6,
IL-1β,
TLR2,
MyD88,
P65,
IκBα,
which
consistent
those
Western
blotting
results.
Moreover,
revealed
promoted
tight
junction-related
proteins
(claudins,
occludin,
ZOs)
inhibited
phosphorylation
MLC,
opposite
trend
after
LPS
treatment.
In
summary,
improved
ability
activation
TLR2/NF-κB/MLCK
pathway
attenuate
injury
intestines
carp.
