Near-Infrared Fluorescence Probe with a New Recognition Moiety for the Specific Detection of Cysteine to Study the Corresponding Physiological Processes in Cells, Zebrafish, and Arabidopsis thaliana

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(15), P. 6030 - 6036

Published: April 3, 2024

Cysteine (Cys), as one of the biological thiols, is related to many physiological and pathological processes in humans plants. Therefore, it necessary develop a sensitive selective method for detection imaging Cys organisms. In this work, novel near-infrared (NIR) fluorescent probe, Probe-Cys, was designed by connecting furancarbonyl, new recognition moiety, with Fluorophore–OH via decomposition IR-806. The use furan moiety anticipated produce more effective fluorescence quenching because electron-donating ability O atom. Probe-Cys has outstanding properties, such group, an emission wavelength infrared region at 710 nm, linear range (0–100 μM), low limit 0.035 μM, good water solubility, excellent sensitivity, selectivity without interference Hcy, GSH, HS–. More importantly, could achieve endogenous reacting stimulant 1,4-dimercaptothreitol (DTT) inhibitor N-ethylmaleimide (NEM) HepG2 cells zebrafish. Ultimately, successfully applied obtain images Arabidopsis thaliana, revealing that content meristematic zone higher than elongation zone, which first time NIR probe used A. thaliana. superior properties exhibit its great potential biosystems explore associated Cys.

Language: Английский

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Construction of ICT-based fluorescence probes for the imaging of biothios

Microchemical Journal, Journal Year: 2025, Volume and Issue: 210, P. 112968 - 112968

Published: Feb. 4, 2025

Language: Английский

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Structurally characterized salamo-based mononuclear Cu(II) complex fluorogenic sensor with high selectivity for CN− and Cys-Cys

Journal of Molecular Structure, Journal Year: 2022, Volume and Issue: 1260, P. 132772 - 132772

Published: March 17, 2022

Language: Английский

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Co‐based Nanozymatic Profiling: Advances Spanning Chemistry, Biomedical, and Environmental Sciences

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(8)

Published: Sept. 19, 2023

Nanozymes, next-generation enzyme-mimicking nanomaterials, have entered an era of rational design; among them, Co-based nanozymes emerged as captivating players over times. been developed and garnered significant attention the past five years. Their extraordinary properties, including regulatable enzymatic activity, stability, multifunctionality stemming from magnetic photothermal conversion effects, cavitation relaxation efficiency, made a rising star. This review presents first comprehensive profiling in chemistry, biology, environmental sciences. The begins by scrutinizing various synthetic methods employed for nanozyme fabrication, such template sol-gel methods, highlighting their distinctive merits chemical standpoint. Furthermore, detailed exploration wide-ranging applications biosensing biomedical therapeutics, well contributions to monitoring remediation is provided. Notably, drawing inspiration state-of-the-art techniques omics, analysis undertaken, employing analogous statistical methodologies provide valuable guidance. To conclude, outlook on challenges prospects presented, spanning microscopic physicochemical mechanisms macroscopic clinical translational applications.

Language: Английский

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Responsive fluorescent probes for cellular microenvironment and redox small biomolecules

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 169, P. 117377 - 117377

Published: Oct. 19, 2023

Language: Английский

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Mitochondria-targetable small molecule fluorescent probes for the detection of cancer-associated biomarkers: A review

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1289, P. 342060 - 342060

Published: Nov. 25, 2023

Language: Английский

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Targeted cysteine and glutathione detection in extra/intracellular systems by copper-based fluorescent imaging probes

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 495, P. 215368 - 215368

Published: Aug. 11, 2023

Language: Английский

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Multi-stimuli-responsive molecular fluorescent probes for bioapplications

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(21), P. 3040 - 3049

Published: Jan. 1, 2023

Stimuli-responsive fluorescent probes have been widely utilized in detecting the physiological and pathological states of living systems. Numerous stimuli-responsive developed due to their advantages good sensitivity, high resolution, contrast signals. In this feature article, progress multi-stimuli-responsive probes, including organic molecules metal complexes, for detection various biomarkers bio-applications is summarized. The article focuses on applications organic-molecule- metal-complex-based molecular biological systems different cancer or other diseases. current challenges potential future directions these are also discussed.

Language: Английский

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Recent Advances in Organometallic NIR Iridium(III) Complexes for Detection and Therapy

Molecules, Journal Year: 2024, Volume and Issue: 29(1), P. 256 - 256

Published: Jan. 3, 2024

Iridium(III) complexes are emerging as a promising tool in the area of detection and therapy due to their prominent photophysical properties, including higher photostability, tunable phosphorescence emission, long-lasting phosphorescence, high quantum yields. In recent years, much effort has been devoted develop novel near-infrared (NIR) iridium(III) improve signal-to-noise ratio enhance tissue penetration. this review, we summarize different classes organometallic NIR for therapy, cyclometalated ligand-enabled NIR-dye-conjugated complexes. Moreover, prospects challenges targeted discussed.

Language: Английский

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Sensing Mechanism of Cysteine Specific Fluorescence Probes and Their Application of Cysteine Recognition

Chemical & Biomedical Imaging, Journal Year: 2024, Volume and Issue: 2(4), P. 250 - 269

Published: Feb. 28, 2024

Due to the biological importance of cysteine (Cys), development organic fluorescence probes for Cys has been a wide, potent, and outstanding research field in most recent years. It used as biomarker treating various diseases; therefore, developing sensing mechanism detecting is very important. In this Review, we focus on summarize specific results exciting literature regarding Cys-specific their applications recognition. Moreover, design strategy can be classified into seven reaction mechanisms, including aromatic substitution rearrangement reaction, cyclization aldehyde, Michael addition Se–N or S–S bond cleavage acrylate, metal complex nucleophilic reaction. all sections, discussions have corresponded which consist emission, color changes, detection limits deal with application recognition sites molecules. Future directions challenges proposed preparation probes.

Language: Английский

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