Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 333, P. 103301 - 103301

Published: Sept. 6, 2024

Language: Английский

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Surface Engineering of Natural Killer Cells with CD44‐targeting Ligands for Augmented Cancer Immunotherapy

Small, Journal Year: 2023, Volume and Issue: 20(24)

Published: Dec. 31, 2023

Abstract Adoptive immunotherapy utilizing natural killer (NK) cells has demonstrated remarkable efficacy in treating hematologic malignancies. However, its clinical intervention for solid tumors is hindered by the limited expression of tumor‐specific antigens. Herein, lipid‐PEG conjugated hyaluronic acid (HA) materials (HA‐PEG‐Lipid) simple ex‐vivo surface coating NK developed 1) lipid‐mediated cellular membrane anchoring via hydrophobic interaction and thereby 2) sufficient presentation CD44 ligand (i.e., HA) onto cancer targeting, without need genetic manipulation. Membrane‐engineered can selectively recognize CD44‐overexpressing through HA‐CD44 affinity subsequently induce situ activation elimination. Therefore, surface‐engineered using HA‐PEG‐Lipid (HANK cells) establish an immune synapse with MIA PaCa‐2 pancreatic cells, triggering “recognition‐activation” mechanism, ultimately eliminating cells. Moreover, mouse xenograft tumor models, administrated HANK demonstrate significant infiltration into tumors, resulting apoptosis/necrosis effective suppression progression metastasis, as compared to gemcitabine. Taken together, biomaterials expedite treatment facilitating a sequential recognition‐activation mechanism suggesting promising approach cell‐mediated immunotherapy.

Language: Английский

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Injectable composite hydrogels embedded with gallium-based liquid metal particles for solid breast cancer treatment via chemo-photothermal combination

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 180, P. 140 - 153

Published: April 10, 2024

Language: Английский

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Optimized Design of Hyaluronic Acid–Lipid Conjugate Biomaterial for Augmenting CD44 Recognition of Surface-Engineered NK Cells

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(3), P. 1959 - 1971

Published: Feb. 20, 2024

Triple-negative breast cancer (TNBC) presents treatment challenges due to a lack of detectable surface receptors. Natural killer (NK) cell-based adaptive immunotherapy is promising because the characteristic anticancer effects killing malignant cells directly by secreting cytokines and lytic granules. To maximize recognition ability NK cells, biomaterial-mediated ex vivo cell engineering has been developed for sufficient membrane immobilization tumor-targeting ligands via hydrophobic anchoring. In this study, we optimized amphiphilic balances coating materials composed CD44-targeting hyaluronic acid (HA)-poly(ethylene glycol) (PEG)-lipid improve TNBC effect. Changes in modular design our material differentiating hydrophilic PEG length incorporating lipid amount into HA backbones precisely regulated nature HA-PEG-lipid conjugates. The biomaterial demonstrated improved anchoring membranes facilitating presentation level onto surfaces. This led enhanced targeting increasing formation immune synapse, thereby augmenting capability specifically toward CD44-positive cells. Our approach addresses solid tumors with deficiency tumor-specific antigens while offering valuable strategy using balance techniques.

Language: Английский

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Engineered inulin-based hybrid biomaterials for augmented immunomodulatory responses

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 340, P. 122311 - 122311

Published: May 23, 2024

Language: Английский

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Tailoring tumor-recognizable hyaluronic acid–lipid conjugates to enhance anticancer efficacies of surface-engineered natural killer cells

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: Dec. 14, 2023

Abstract Natural killer (NK) cells have clinical advantages in adoptive cell therapy owing to their inherent anticancer efficacy and ability identify eliminate malignant tumors. However, insufficient cancer-targeting ligands on NK surfaces often inhibit immunotherapeutic performance, especially immunosuppressive tumor microenvironment. To facilitate recognition subsequent function of cells, we developed hyaluronic acid (HA, target CD44 overexpressed onto cancer cells)-poly(ethylene glycol) (PEG, cytoplasmic penetration blocker)-Lipid (molecular anchor for membrane decoration through hydrophobic interaction) conjugates biomaterial-mediated ex vivo surface engineering. Among these major compartments (i.e., Lipid, PEG HA), optimization lipid anchors (in terms chemical structure intrinsic amphiphilicity) is the most important design parameter modulate interaction with dynamic membranes. Here, three different types including 1,2-dimyristoyl-sn-glycero-3-phosphati-dylethanolamine (C14:0), 1,2-distearoyl-sn-glycero-3-phosphatidylethanolamine (DSPE, C18:0), cholesterol were evaluated maximize coating associated performance surface-engineered (HALipid-NK cells). Our results demonstrated that coated HA-PEG-DSPE exhibited significantly enhanced efficacies toward MDA-MB-231 breast without an off-target effect human fibroblasts specifically via increased prolonged duration HA surfaces, thereby improving HA-CD44 recognition. These suggest our HALipid-NK tumor-recognizable could be further utilized various immunotherapies. Graphical

Language: Английский

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Biomaterial-Mediated Exogenous Facile Coating of Natural Killer Cells for Enhancing Anticancer Efficacy toward Hepatocellular Carcinoma

Bioconjugate Chemistry, Journal Year: 2023, Volume and Issue: 34(10), P. 1789 - 1801

Published: Sept. 20, 2023

Natural killer (NK) cells exhibit a good therapeutic efficacy against various malignant cancer cells. However, the of plain NK is relatively low due to inadequate selectivity for Therefore, enhance targeting and anticancer cells, we have rationally designed biomaterial-mediated ex vivo surface engineering technique membrane decoration recognition ligands onto Our lipid conjugate biomaterial contains three major functional moieties: (1) 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) cell anchoring, (2) polyethylene glycol intracellular penetration blocker, (3) lactobionic acid (LBA) recognition. The was successfully applied surfaces (LBA-NK) functionalities, especially toward asialoglycoprotein receptor (ASGPR)-overexpressing hepatocellular carcinoma. Highly efficient homogeneous editing achieved with simple coating process while maintaining intrinsic properties LBA-NK showed potential ASGPR-mediated tumor binding (through LBA-ASGPR interaction) thereby significantly augmented efficacies HepG2 liver Thus, can be novel strategy treatment cancers via facilitated immune synapse interactions in comparison currently available therapies.

Language: Английский

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A Facile Approach To Develop Ion Pair Micelles Satellited Freshly Derived Neutrophils For Targeted Tumor Therapy

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Immune cells show enormous potential for targeted nanoparticle delivery due to their intrinsic tumor-homing skills. However, the immune can internalize nanoparticles, leading cellular functional impairments, degradation of and delayed release drugs from cells. To address these issues, this study introduces an approach synthesis freshly derived neutrophils (NUs)-based nanocarriers system where NUs are surfaced by dialdehyde alginate-coated self-assembled micelles loaded with mitoxantrone (MIT) indocyanine green (ICG) (i.e., dA(MI@IPM)s) stimuli-responsive tumor-targeted therapy. Here, dA(MI@IPM)s not internalized NUs, but they anchored on membrane via distearoylphosphatidylethanolamine-polyethylene glycol-polyethylenimine anchors. Owing natural recruitment ability tumor microenvironment, NUs-anchored accumulation is higher at site than free dA(MI@IPM)s, readily detach get in The disassembles inside cancer upon near-infrared irradiation photosensitizing effect ICG, releasing MIT significantly inhibiting growth. This simple fast prepare, opening up exciting possibilities personalized treatment using patient's autologous NUs.

Language: Английский

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Research Progress of NK Cells in Glioblastoma Treatment

OncoTargets and Therapy, Journal Year: 2025, Volume and Issue: Volume 18, P. 87 - 106

Published: Jan. 1, 2025

NK cells are a type of antitumor immune cell with promising clinical application, following T cells. The activity is primarily regulated by their surface receptors and microenvironment. In gliomas, the tumor microenvironment exerts strong immunosuppressive effect, which significantly reduces efficacy immunotherapy. Therefore, this review aims to discuss latest research on role in glioma immunotherapy, focusing aspects such as development, function, localization. It summarizes information compounds, monoclonal antibodies, cytokine therapies targeting while emphasizing current status trends gene-modified treatment. Additionally, it explores molecular mechanisms underlying escape cells, providing theoretical foundation new perspectives for cell-based immunotherapy gliomas.

Language: Английский

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Recent TRAIL Engineering Strategies for Precise Strike Therapy Against Tumor

Biomaterials Research, Journal Year: 2025, Volume and Issue: 29

Published: Jan. 1, 2025

Effective drug delivery relies on the selection of suitable carriers, which is crucial for protein-based therapeutics such as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). One key advantages TRAIL its ability to selectively induce apoptosis in cancer cells excluding healthy tissues by binding death receptors DR4 and DR5, are highly expressed various cells. Despite this promise, clinical application has been limited short half-life, stability, inefficient sites. To overcome currently available engineering approaches, a series sophisticated strategies required: (a) design biomaterial-mediated carriers enhanced targeting efficacy, particularly via optimizing selected materials, composition, formulation, surface modulation. Moreover, (b) development genetically modified cellular products augmented secretion toward microenvironments (c) cell techniques immobilization onto infusible populations also discussed present review. Among these living cell-based offer distinct advantage systemically administered TRAIL-functionalized capturing circulating bloodstream, thereby preventing secondary formation. This review provides insight into novel platforms, discusses considerations translation, suggests future directions complementary advance field TRAIL-based therapeutics.

Language: Английский

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