Networked Cluster Formation via Trigonal Lipid Modules for Augmented Ex Vivo NK Cell Priming DOI Open Access
Jaewon Park, Sungjun Kim, Ashok Kumar Jangid

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1556 - 1556

Published: Jan. 26, 2024

Current cytokine-based natural killer (NK) cell priming techniques have exhibited limitations such as the deactivation of biological signaling molecules and subsequent insufficient maturation population during mass cultivation processes. In this study, we developed an amphiphilic trigonal 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-polyethylene glycol (PEG) material to assemble NK clusters via multiple hydrophobic lipid insertions into cellular membranes. Our conjugate-mediated ex vivo sufficiently augmented structural modulation clusters, facilitated diffusional signal exchanges, finally activated with clusters. Without any inhibition in exchanges intrinsic proliferative efficacy cells, effectively prime produced increased interferon-gamma, especially early culture periods. conclusion, present study demonstrates that our novel conjugates could serve a promising alternative for future production.

Language: Английский

Surface Engineering of Natural Killer Cells with CD44‐targeting Ligands for Augmented Cancer Immunotherapy DOI
Sungjun Kim, Shujin Li, Ashok Kumar Jangid

et al.

Small, Journal Year: 2023, Volume and Issue: 20(24)

Published: Dec. 31, 2023

Abstract Adoptive immunotherapy utilizing natural killer (NK) cells has demonstrated remarkable efficacy in treating hematologic malignancies. However, its clinical intervention for solid tumors is hindered by the limited expression of tumor‐specific antigens. Herein, lipid‐PEG conjugated hyaluronic acid (HA) materials (HA‐PEG‐Lipid) simple ex‐vivo surface coating NK developed 1) lipid‐mediated cellular membrane anchoring via hydrophobic interaction and thereby 2) sufficient presentation CD44 ligand (i.e., HA) onto cancer targeting, without need genetic manipulation. Membrane‐engineered can selectively recognize CD44‐overexpressing through HA‐CD44 affinity subsequently induce situ activation elimination. Therefore, surface‐engineered using HA‐PEG‐Lipid (HANK cells) establish an immune synapse with MIA PaCa‐2 pancreatic cells, triggering “recognition‐activation” mechanism, ultimately eliminating cells. Moreover, mouse xenograft tumor models, administrated HANK demonstrate significant infiltration into tumors, resulting apoptosis/necrosis effective suppression progression metastasis, as compared to gemcitabine. Taken together, biomaterials expedite treatment facilitating a sequential recognition‐activation mechanism suggesting promising approach cell‐mediated immunotherapy.

Language: Английский

Citations

18
Optimized Design of Hyaluronic Acid–Lipid Conjugate Biomaterial for Augmenting CD44 Recognition of Surface-Engineered NK Cells DOI
Hee Won Park, Wonjeong Lee, Sungjun Kim

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(3), P. 1959 - 1971

Published: Feb. 20, 2024

Triple-negative breast cancer (TNBC) presents treatment challenges due to a lack of detectable surface receptors. Natural killer (NK) cell-based adaptive immunotherapy is promising because the characteristic anticancer effects killing malignant cells directly by secreting cytokines and lytic granules. To maximize recognition ability NK cells, biomaterial-mediated ex vivo cell engineering has been developed for sufficient membrane immobilization tumor-targeting ligands via hydrophobic anchoring. In this study, we optimized amphiphilic balances coating materials composed CD44-targeting hyaluronic acid (HA)-poly(ethylene glycol) (PEG)-lipid improve TNBC effect. Changes in modular design our material differentiating hydrophilic PEG length incorporating lipid amount into HA backbones precisely regulated nature HA-PEG-lipid conjugates. The biomaterial demonstrated improved anchoring membranes facilitating presentation level onto surfaces. This led enhanced targeting increasing formation immune synapse, thereby augmenting capability specifically toward CD44-positive cells. Our approach addresses solid tumors with deficiency tumor-specific antigens while offering valuable strategy using balance techniques.

Language: Английский

Citations

6
Engineered inulin-based hybrid biomaterials for augmented immunomodulatory responses DOI
Ashok Kumar Jangid,

Kyung Mu Noh,

Sung Jun Kim

et al.

Carbohydrate Polymers, Journal Year: 2024, Volume and Issue: 340, P. 122311 - 122311

Published: May 23, 2024

Language: Английский

Citations

5
Phenylboronic acid-functionalized biomaterials for improved cancer immunotherapy via sialic acid targeting DOI
Ashok Kumar Jangid, Kyobum Kim

Advances in Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 333, P. 103301 - 103301

Published: Sept. 6, 2024

Language: Английский

Citations

5
Tailoring tumor-recognizable hyaluronic acid–lipid conjugates to enhance anticancer efficacies of surface-engineered natural killer cells DOI Creative Commons
Chae Eun Lee, Sungjun Kim, Hee Won Park

et al.

Nano Convergence, Journal Year: 2023, Volume and Issue: 10(1)

Published: Dec. 14, 2023

Abstract Natural killer (NK) cells have clinical advantages in adoptive cell therapy owing to their inherent anticancer efficacy and ability identify eliminate malignant tumors. However, insufficient cancer-targeting ligands on NK surfaces often inhibit immunotherapeutic performance, especially immunosuppressive tumor microenvironment. To facilitate recognition subsequent function of cells, we developed hyaluronic acid (HA, target CD44 overexpressed onto cancer cells)-poly(ethylene glycol) (PEG, cytoplasmic penetration blocker)-Lipid (molecular anchor for membrane decoration through hydrophobic interaction) conjugates biomaterial-mediated ex vivo surface engineering. Among these major compartments (i.e., Lipid, PEG HA), optimization lipid anchors (in terms chemical structure intrinsic amphiphilicity) is the most important design parameter modulate interaction with dynamic membranes. Here, three different types including 1,2-dimyristoyl-sn-glycero-3-phosphati-dylethanolamine (C14:0), 1,2-distearoyl-sn-glycero-3-phosphatidylethanolamine (DSPE, C18:0), cholesterol were evaluated maximize coating associated performance surface-engineered (HALipid-NK cells). Our results demonstrated that coated HA-PEG-DSPE exhibited significantly enhanced efficacies toward MDA-MB-231 breast without an off-target effect human fibroblasts specifically via increased prolonged duration HA surfaces, thereby improving HA-CD44 recognition. These suggest our HALipid-NK tumor-recognizable could be further utilized various immunotherapies. Graphical

Language: Английский

Citations

13
Exploration of the role of immune cells and cell therapy in hepatocellular carcinoma DOI Creative Commons
Tao Zhang, Chongyang Ren, Zhanyu Yang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Hepatocellular carcinoma stands as one of the foremost contributors to cancer-associated fatalities globally, and limitations traditional treatment methods have prompted researchers explore new therapeutic options. Recently, cell therapy has emerged a promising approach for HCC, showing significant potential in improving patient outcomes. This review article explores use covering different types, mechanisms behind their effectiveness, recent advancements clinical trials, ongoing challenges. aims provide insightful perspectives future research applications treating HCC by synthesizing current knowledge.

Language: Английский

Citations

0
Ex Vivo Surface Decoration of Phenylboronic Acid onto Natural Killer Cells for Sialic Acid-Mediated Versatile Cancer Cell Targeting DOI
Ashok Kumar Jangid, Sungjun Kim, Hee Won Park

et al.

Biomacromolecules, Journal Year: 2023, Volume and Issue: 25(1), P. 222 - 237

Published: Dec. 22, 2023

Phenylboronic acid (PBA) has been highly acknowledged as a significant cancer recognition moiety in sialic acid-overexpressing cells. In this investigation, lipid-mediated biomaterial integrated PBA molecules onto the surface of natural killer (NK) cells to make receptor-mediated immune cell therapeutic module. Therefore, 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-conjugated di–PEG–PBA (DSPEPEG-di(PEG–PBA) was synthesized. The DSPEPEG-di(PEG–PBA) exhibited high affinity for (SA), confirmed by fluorescence spectroscopy at pH 6.5 and 7.4. successfully anchored NK surfaces (PBA-NK), maintains intrinsic properties such viability, ligand availability (FasL & TRAIL), cytokine secretion response LPS. anticancer efficacy PBA-NK evaluated against 2D (MDA-MB-231, HepG2, HCT-116) 3D tumor spheroids MDA-MB-231 greatly enhanced effects SA-overexpressing Thus, represent new strategy immunotherapy.

Language: Английский

Citations

9
Membrane-immobilized Gemcitabine for Cancer-Targetable NK Cell Surface Engineering DOI

Kyung Mu Noh,

Ashok Kumar Jangid, Jaewon Park

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(46), P. 12087 - 12102

Published: Jan. 1, 2024

Although natural killer (NK) cell-based adoptive cell transfer (ACT) has shown promise in cancer immunotherapy, its efficacy against solid tumors is limited the immunosuppressive tumor microenvironment (TME). Combinatorial therapies involving chemotherapeutic drugs such as gemcitabine (Gem) and NK cells have been developed to modulate TME; however, their clinical application constrained by low drug delivery efficiency significant off-target toxicity. In this study, we membrane-immobilized Gem conjugates (

Language: Английский

Citations

2
Advancements of engineered live oncolytic biotherapeutics (microbe/virus/cells): Preclinical research and clinical progress DOI

Zhengzhuo Qi,

Junmou Gu,

Lihang Qu

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 375, P. 209 - 235

Published: Sept. 11, 2024

Language: Английский

Citations

0
Networked Cluster Formation via Trigonal Lipid Modules for Augmented Ex Vivo NK Cell Priming DOI Open Access
Jaewon Park, Sungjun Kim, Ashok Kumar Jangid

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1556 - 1556

Published: Jan. 26, 2024

Current cytokine-based natural killer (NK) cell priming techniques have exhibited limitations such as the deactivation of biological signaling molecules and subsequent insufficient maturation population during mass cultivation processes. In this study, we developed an amphiphilic trigonal 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-polyethylene glycol (PEG) material to assemble NK clusters via multiple hydrophobic lipid insertions into cellular membranes. Our conjugate-mediated ex vivo sufficiently augmented structural modulation clusters, facilitated diffusional signal exchanges, finally activated with clusters. Without any inhibition in exchanges intrinsic proliferative efficacy cells, effectively prime produced increased interferon-gamma, especially early culture periods. conclusion, present study demonstrates that our novel conjugates could serve a promising alternative for future production.

Language: Английский

Citations

0