Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(2)

Published: Feb. 1, 2024

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of cell origin and change functions phenotypes other cells. These features indicate strong biomarker therapeutic potential have generated broad interest, as evidenced by steady year-on-year increase in numbers scientific publications about EVs. Important advances been made EV metrology understanding applying biology. However, hurdles remain to realising EVs domains ranging from basic biology clinical applications due challenges nomenclature, separation non-vesicular extracellular particles, characterisation functional studies. To address opportunities this rapidly evolving field, International Society for Vesicles (ISEV) updates its 'Minimal Information Studies Vesicles', which was first published 2014 then 2018 MISEV2014 MISEV2018, respectively. The goal current document, MISEV2023, is provide researchers with an updated snapshot available approaches advantages limitations production, multiple sources, including culture, body fluids solid tissues. In addition presenting latest art principles research, document also covers advanced techniques that are currently expanding boundaries field. MISEV2023 includes new sections on release uptake a brief discussion vivo study Compiling feedback ISEV expert task forces more than 1000 researchers, conveys research facilitate robust discoveries move field forward even rapidly.

Language: Английский

---

The matrix in cancer

Nature reviews. Cancer, Journal Year: 2021, Volume and Issue: 21(4), P. 217 - 238

Published: Feb. 15, 2021

Language: Английский

---

Exosomes as a new frontier of cancer liquid biopsy

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: Feb. 18, 2022

Abstract Liquid biopsy, characterized by minimally invasive detection through biofluids such as blood, saliva, and urine, has emerged a revolutionary strategy for cancer diagnosis prognosis prediction. Exosomes are subset of extracellular vesicles (EVs) that shuttle molecular cargoes from donor cells to recipient play crucial role in mediating intercellular communication. Increasing studies suggest exosomes have great promise serve novel biomarkers liquid since large quantities enriched body fluids involved numerous physiological pathological processes. However, the further clinical application been greatly restrained lack high-quality separation component analysis methods. This review aims provide comprehensive overview on conventional technologies exosome isolation, characterization content detection. Additionally, roles serving potential biopsy diagnosis, treatment monitoring, prediction summarized. Finally, prospects challenges applying exosome-based precision medicine evaluated.

Language: Английский

---

Exosomes as mediators of intercellular crosstalk in metabolism

Cell Metabolism, Journal Year: 2021, Volume and Issue: 33(9), P. 1744 - 1762

Published: Sept. 1, 2021

Language: Английский

---

Extracellular vesicles and nanoparticles: emerging complexities

Trends in Cell Biology, Journal Year: 2023, Volume and Issue: 33(8), P. 667 - 681

Published: Feb. 1, 2023

The study of extracellular vesicles (EVs) and nanoparticles (NPs) is rapidly expanding because recent discoveries have revealed a much greater complexity diversity than was appreciated only few years ago. New types EVs NPs recently been described. Proteins nucleic acids previously thought to be packaged in exosomes appear more enriched different two identified amembranous NPs, exomeres supermeres. Thus, our understanding the cell biology intercellular communication facilitated by release state flux. In this review, we describe highlight advances, present major outstanding questions.

Language: Английский

---

Isolation and characterization of exosomes for cancer research

Journal of Hematology & Oncology, Journal Year: 2020, Volume and Issue: 13(1)

Published: Nov. 10, 2020

Abstract Exosomes are a subset of extracellular vesicles that carry specific combinations proteins, nucleic acids, metabolites, and lipids. Mounting evidence suggests exosomes participate in intercellular communication act as important molecular vehicles the regulation numerous physiological pathological processes, including cancer development. released by various cell types under both normal conditions, they can be found multiple bodily fluids. Moreover, carrying wide variety macromolecules provide window into altered cellular or tissue states. Their presence biological fluids renders them an attractive, minimally invasive approach for liquid biopsies with potential biomarkers diagnosis, prediction, surveillance. Due to their biocompatibility low immunogenicity cytotoxicity, have clinical applications development innovative therapeutic approaches. Here, we summarize recent advances technologies exosome isolation research. We outline functions regulating tumor metastasis, drug resistance, immune modulation context Finally, discuss prospects challenges exosome-based therapeutics.

Language: Английский

---

Extracellular vesicles as tools and targets in therapy for diseases

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Feb. 5, 2024

Abstract Extracellular vesicles (EVs) are nano-sized, membranous structures secreted into the extracellular space. They exhibit diverse sizes, contents, and surface markers ubiquitously released from cells under normal pathological conditions. Human serum is a rich source of these EVs, though their isolation proteins non-EV lipid particles poses challenges. These transport various cellular components such as proteins, mRNAs, miRNAs, DNA, lipids across distances, influencing numerous physiological events, including those within tumor microenvironment (TME). Their pivotal roles in communication make EVs promising candidates for therapeutic agents, drug delivery systems, disease biomarkers. Especially cancer diagnostics, EV detection can pave way early identification offers potential diagnostic Moreover, subtypes emerging targeted tools, highlighting clinical significance. The need non-invasive biomarkers to monitor biological processes purposes remains unfulfilled. Tapping unique composition could unlock advanced avenues future. In this review, we discuss detail conditions, cancers (encompassing head neck, lung, gastric, breast, hepatocellular carcinoma), neurodegenerative disorders, diabetes, viral infections, autoimmune renal diseases, emphasizing advancements molecular diagnostics delivery.

Language: Английский

---

Shedding Light on Extracellular Vesicle Biogenesis and Bioengineering

Advanced Science, Journal Year: 2020, Volume and Issue: 8(1)

Published: Nov. 27, 2020

Abstract Extracellular vesicles (EVs) are biocompatible, nano‐sized secreted containing many types of biomolecules, including proteins, RNAs, DNAs, lipids, and metabolites. Their low immunogenicity ability to functionally modify recipient cells by transferring diverse bioactive constituents make them an excellent candidate for a next‐generation drug delivery system. Here, the recent advances in EV biology emerging strategies bioengineering summarized, prospects clinical translation bioengineered EVs challenges be overcome discussed.

Language: Английский

---

The role of extracellular vesicles in cancer

Cell, Journal Year: 2023, Volume and Issue: 186(8), P. 1610 - 1626

Published: April 1, 2023

Language: Английский

---

Liquid biopsy in lung cancer: significance in diagnostics, prediction, and treatment monitoring

Molecular Cancer, Journal Year: 2022, Volume and Issue: 21(1)

Published: Jan. 20, 2022

Primary lung cancer is one of the most common malignant tumors in China. Approximately 60% patients have distant metastasis at initial diagnosis, so it necessary to find new tumor markers for early diagnosis and individualized treatment. Tumor contribute play important roles detection treatment, as well precision medicine, efficacy monitoring, prognosis prediction. The pathological small biopsy specimens determines whether there are cells type. Because traumatic compliance with multiple biopsies poor, liquid has become a hot research direction. Liquid advantageous because they nontraumatic, easy obtain, reflect overall state tumor, allow real-time monitoring. At present, mainly include circulating cells, DNA, exosomes, microRNA, RNA, platelets, endothelial cells. This review introduces progress clinical application prospect technology cancer.

Language: Английский

---