Extracellular vesicle-associated DNA: ten years since its discovery in human blood DOI Creative Commons
Thupten Tsering, Amélie Nadeau,

T.-C. Wu

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(9)

Published: Sept. 12, 2024

Abstract Extracellular vesicles (EVs) have emerged as key players in intercellular communication, facilitating the transfer of crucial cargo between cells. Liquid biopsy, particularly through isolation EVs, has unveiled a rich source potential biomarkers for health and disease, encompassing proteins nucleic acids. A milestone this exploration occurred decade ago with identification extracellular vesicle-associated DNA (EV-DNA) bloodstream patient diagnosed pancreatic cancer. Subsequent years witnessed substantial advancements, deepening our insights into molecular intricacies EV-DNA emission, detection, analysis. Understanding complexities surrounding release addressing challenges inherent research are pivotal steps toward enhancing liquid biopsy-based strategies. These strategies, detection monitoring various pathological conditions, cancer, rely on comprehensive understanding why how is released. In review, we aim to provide thorough summary decade’s worth EV-DNA. We will delve diverse mechanisms its biomarker, functional capabilities, discordant findings field, hurdles hindering clinical application. Looking ahead next decade, envision that advancements EV techniques, coupled improved standardization data sharing, catalyze development novel strategies exploiting both therapeutic targets.

Language: Английский

Recent advances in extracellular vesicles for therapeutic cargo delivery DOI Creative Commons
Hyo In Kim, Jin Bong Park, Yin Zhu

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(4), P. 836 - 849

Published: April 1, 2024

Abstract Exosomes, which are nanosized vesicles secreted by cells, attracting increasing interest in the field of biomedical research due to their unique properties, including biocompatibility, cargo loading capacity, and deep tissue penetration. They serve as natural signaling agents intercellular communication, inherent ability carry proteins, lipids, nucleic acids endows them with remarkable therapeutic potential. Thus, exosomes can be exploited for diverse applications, chemotherapy, gene therapy, photothermal therapy. Moreover, capacity homotypic targeting self-recognition provides opportunities personalized medicine. Despite advantages novel agents, there several challenges optimizing efficiency structural stability defining exosome origins. Future should include development large-scale, quality-controllable production methods, refinement drug strategies, extensive vivo studies clinical trials. unresolved difficulties, use efficient, stable, safe delivery systems is an interesting area research. Therefore, this review describes summarizes cutting-edge published high-impact journals that have introduced or enhanced effects using a system past 2 years. We provide informative overview current state research, highlighting properties applications exosomes. also emphasize future directions, underscoring importance addressing key issues field. With review, we encourage researchers further develop exosome-based drugs application, such may among most promising next-generation therapeutics.

Language: Английский

Citations

75
Engineering Extracellular Vesicles as Delivery Systems in Therapeutic Applications DOI Creative Commons
Liwei Wang, Di Wang, Zhaoming Ye

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 20, 2023

Extracellular vesicles (EVs) are transport secreted by living cells and released into the extracellular environment. Recent studies have shown that EVs serve as "messengers" in intercellular inter-organismal communication, both normal pathological processes. EVs, natural nanocarriers, can deliver bioactivators therapy with their endogenous properties. This review article describes engineering of sources, isolation method, cargo loading, boosting approach, adjustable targeting EVs. Furthermore, summarizes recent progress made EV-based delivery systems applications, including cancer, cardiovascular diseases, liver, kidney, nervous system COVID-19 emphasizes obstacles challenges therapies possible strategies.

Language: Английский

Citations

60
A beginner's guide to study extracellular vesicles in human blood plasma and serum DOI Creative Commons
Rienk Nieuwland, Pia Siljander

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Blood is the most commonly used body fluid for obtaining and studying extracellular vesicles (EVs). While blood a standard choice clinical analysis, using as source of EVs introduces multiple layers complexity. At Extracellular Vesicle Workshop organized by International Society Vesicles in Helsinki (2022), it became evident that beginner researchers lack trustworthy information on how to initiate their research avoid common pitfalls. This educational guide explains composition frequently terminology blood, provides guidelines collection, preparation plasma serum. It also basic principles isolating detecting while considering blood‐related factors. The goal this assist beginners offering concise evidence‐based introduction current knowledge available resources study EVs.

Language: Английский

Citations

37
Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade DOI Creative Commons
Saeid Moghassemi, Arezoo Dadashzadeh, Maria João Sousa

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 36, P. 126 - 156

Published: March 2, 2024

Small extracellular vesicles (sEVs) are known to be secreted by a vast majority of cells. These sEVs, specifically exosomes, induce specific cell-to-cell interactions and can activate signaling pathways in recipient cells through fusion or interaction. nanovesicles possess several desirable properties, making them ideal for regenerative medicine nanomedicine applications. properties include exceptional stability, biocompatibility, wide biodistribution, minimal immunogenicity. However, the practical utilization particularly clinical settings at large scale, is hindered expensive procedures required their isolation, limited circulation lifetime, suboptimal targeting capacity. Despite these challenges, sEVs have demonstrated remarkable ability accommodate various cargoes found extensive applications biomedical sciences. To overcome limitations broaden potential applications, researchers should strive deepen understanding current loading, characterization techniques. Additionally, acquiring fundamental knowledge about origins employing state-of-the-art methodologies expand research scope. This review provides comprehensive overview exosome-based strategies diverse domains, encompassing cancer therapy, immunotherapy, biomarker Furthermore, we emphasize immense exosomes medicine.

Language: Английский

Citations

30
The biology and function of extracellular vesicles in immune response and immunity DOI
Raghu Kalluri

Immunity, Journal Year: 2024, Volume and Issue: 57(8), P. 1752 - 1768

Published: Aug. 1, 2024

Language: Английский

Citations

22
Advances in Extracellular Vesicle Research Over the Past Decade: Source and Isolation Method are Connected with Cargo and Function DOI Creative Commons
Rodolphe Poupardin, Martin Wolf, Nicole Maeding

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(19)

Published: Jan. 25, 2024

Abstract The evolution of extracellular vesicle (EV) research has introduced nanotechnology into biomedical cell communication science while recognizing what is formerly considered “dust” as constituting an entirely new universe signaling particles. To display the global EV landscape, a systematic review 20 364 original articles selected from all 40 684 EV‐related records identified in PubMed 2013–2022 performed. Machine‐learning used to categorize high‐dimensional data and further dissected significant associations between source, isolation method, cargo, function. Unexpected correlations these four categories indicate prevalent experimental strategies based on cargo connectivity with function interest being associated certain sources or strategies. Conceptually relevant association size‐based protein uptake will guide strategic conclusions enhancing future product development. Based this study, open‐source database built facilitate analysis conventional AI tools identify additional causative interest.

Language: Английский

Citations

20
Exosomal circRNAs: Novel biomarkers and therapeutic targets for urinary tumors DOI
Qiang Liu, Shenglong Li

Cancer Letters, Journal Year: 2024, Volume and Issue: 588, P. 216759 - 216759

Published: Feb. 28, 2024

Language: Английский

Citations

20
Non-coding RNAs as therapeutic targets and biomarkers in ischaemic heart disease DOI
Andrea Caporali, Maryam Anwar, Yvan Devaux

et al.

Nature Reviews Cardiology, Journal Year: 2024, Volume and Issue: 21(8), P. 556 - 573

Published: March 18, 2024

Language: Английский

Citations

20
Emerging role of exosomes in cancer therapy: progress and challenges DOI Creative Commons
Jiale Li,

Jiachong Wang,

Zigui Chen

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 13, 2025

This review highlights recent progress in exosome-based drug delivery for cancer therapy, covering exosome biogenesis, cargo selection mechanisms, and their application across multiple types. As small extracellular vesicles, exosomes exhibit high biocompatibility low immunogenicity, making them ideal vehicles capable of efficiently targeting cells, minimizing off-target damage side effects. aims to explore the potential with a focus on applications chemotherapy, gene immunomodulation. Additionally, challenges related production standardization are analyzed, highlighting importance addressing these issues clinical application. In conclusion, systems offer promising future therapies. Further research should aim enhance efficiency facilitate translation, paving way innovative treatment strategies.

Language: Английский

Citations

10
Harnessing the tumor microenvironment: targeted cancer therapies through modulation of epithelial-mesenchymal transition DOI Creative Commons
Antonino Glaviano,

Hannah Lau,

Lukas M. Carter

et al.

Journal of Hematology & Oncology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 13, 2025

The tumor microenvironment (TME) is integral to cancer progression, impacting metastasis and treatment response. It consists of diverse cell types, extracellular matrix components, signaling molecules that interact promote growth therapeutic resistance. Elucidating the intricate interactions between cells TME crucial in understanding progression challenges. A critical process induced by epithelial-mesenchymal transition (EMT), wherein epithelial acquire mesenchymal traits, which enhance their motility invasiveness progression. By targeting various components TME, novel investigational strategies aim disrupt TME's contribution EMT, thereby improving efficacy, addressing resistance, offering a nuanced approach therapy. This review scrutinizes key players emphasizing avenues therapeutically components. Moreover, article discusses implications for resistance mechanisms highlights current toward modulation along with potential caveats.

Language: Английский

Citations

10